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Species: Homo sapiens
Genetic Insert: Enhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173956 Copy
Species: Homo sapiens
Genetic Insert: Enhancer region 4 (S4) from SOX9 enhancer cluster EC1.25
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173958 Copy
Species: Homo sapiens
Genetic Insert: Minimal enhancer region 1 (min1) from SOX9 enhancer cluster EC1.45
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5016; Vector Backbone:pGL3-Promoter, GenBank: U47298.2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991838
Proper citation: RRID:Addgene_173950 Copy
Vector Backbone Description: Backbone Size:3052; Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34608327
Proper citation: RRID:Addgene_174039 Copy
Species: Synthetic
Genetic Insert: AcrIIA4
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2; Vector Types:In vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34735006
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.11.07.372151v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_174034 Copy
Species: Synthetic
Genetic Insert: AcrIIC1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2; Vector Types:In vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34735006
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.11.07.372151v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_174035 Copy
Species: Synthetic
Genetic Insert: AcrIIC3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2; Vector Types:In vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34735006
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.11.07.372151v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_174036 Copy
Species: Synthetic
Genetic Insert: p2A-Venus-PEST
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Zebrafish knock-in tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34495314
Comments: Not all restriction sites in the multiple cloning sites are single cutters, depending on the fluorescent protein sequence of the insert within the plasmid.
Proper citation: RRID:Addgene_174030 Copy
Species: Synthetic
Genetic Insert: p2A-eGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Zebrafish knock-in tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34495314
Comments: Not all restriction sites in the multiple cloning sites are single cutters, depending on the fluorescent protein sequence of the insert within the plasmid.
Proper citation: RRID:Addgene_174028 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Zebrafish knock-in tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34495314
Comments: Not all restriction sites in the multiple cloning sites are single cutters, depending on the fluorescent protein sequence of the insert within the plasmid.
Proper citation: RRID:Addgene_174024 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Zebrafish knock-in tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34495314
Comments: Not all restriction sites in the multiple cloning sites are single cutters, depending on the fluorescent protein sequence of the insert within the plasmid.
Proper citation: RRID:Addgene_174025 Copy
Species: Synthetic
Genetic Insert: p2A-QF2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Zebrafish knock-in tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34495314
Comments: Not all restriction sites in the multiple cloning sites are single cutters, depending on the fluorescent protein sequence of the insert within the plasmid.
Proper citation: RRID:Addgene_174026 Copy
Vector Backbone Description: Backbone Size:13430; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32976518
Comments: Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants
https://academic.oup.com/plphys/article/146/2/323/6107098
Please note there are two mutations in HygR- E180D and A95S. Depositor confirms these do not affect plasmid function.
Proper citation: RRID:Addgene_174050 Copy
Species: Drosophila melanogaster
Genetic Insert: calx2
Vector Backbone Description: Backbone Size:8780; Vector Backbone:pCaspeRhs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15694324
Proper citation: RRID:Addgene_1741 Copy
Species: Synthetic
Genetic Insert: Superfolder GFP
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:5653; Vector Backbone:pRBC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31243963
Proper citation: RRID:Addgene_174076 Copy
Species: Synthetic
Genetic Insert: Superfolder GFP
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:5653; Vector Backbone:pRBC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31243963
Proper citation: RRID:Addgene_174077 Copy
Species: Homo sapiens
Genetic Insert: Codon optimized VPS13D
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:6000; Vector Backbone:pcDNA3.4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33891013
Proper citation: RRID:Addgene_174110 Copy
Species: Mus musculus
Genetic Insert: Let-7g mutant
Vector Backbone Description: Backbone Marker:Jacks Lab; Backbone Size:9200; Vector Backbone:Puro.Cre; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18308936
Comments: Puro.let7gsm.Cre was generated by two rounds site-directed mutagenesis of Puro.let7g.Cre with mut1F (5’-GATTCCAGGCTGACCTAGTAGTTTGTACAGTTTGAGGGTCTATGATAC-3’), mut1R (5’-GTATCATAGACCCTCAAACTGTACAAACTACTAGGTCAGCCTGGAATC-3’), mut2F (5’-CAGGAGATAACTGTACAGGCCACTGGGTTGCCAGGAAC-3’), and mut2R (5’-GTTCCTGGCAACCCAGTGGCCTGTACAGTTATCTCCTG-3’).
For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).
Proper citation: RRID:Addgene_17410 Copy
Species: Mus musculus
Genetic Insert: HMGA2
Vector Backbone Description: Backbone Marker:Available at Addgene (#1766); Backbone Size:4900; Vector Backbone:pBabe-Zeo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18308936
Comments: The ORF of human HMGA2 was amplified using HMGA2F (5’-TAAGGATCCATGAGCGCACGCGGTGAG-3’) and HMGA2R (5’- GCGGAATTCCTAATCCTCCTCTGCGGACTCTTG-3’). The product was cut with BamHI and EcoRI and cloned into pBabe.Zeo.
Proper citation: RRID:Addgene_17411 Copy
Species: Homo sapiens
Genetic Insert: Codon optimized VPS13D
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:6000; Vector Backbone:pcDNA3.4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33891013
Proper citation: RRID:Addgene_174108 Copy
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