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Species: Rattus norvegicus
Genetic Insert: mammalian target of rapamycin-R2505P
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20190810
Proper citation: RRID:Addgene_26038 Copy
Species: Rattus norvegicus
Genetic Insert: mammalian target of rapamycin-S2215Y
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20190810
Proper citation: RRID:Addgene_26037 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:2946; Vector Backbone:p3E; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23462469
Comments: "p3E" refers to 3' entry clones flanked by R2-L3.
Proper citation: RRID:Addgene_26031 Copy
Genetic Insert: N terminal avi tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26008 Copy
Species: Homo sapiens
Genetic Insert: LEMD3 LEM domain containing 3
Vector Backbone Description: Backbone Size:3919; Vector Backbone:pSVK3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11896184
Proper citation: RRID:Addgene_26002 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 2
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19883615
Proper citation: RRID:Addgene_25969 Copy
Species: Mus musculus
Genetic Insert: Kinase suppressor of Ras 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19883615
Proper citation: RRID:Addgene_25968 Copy
Species: Homo sapiens
Genetic Insert: B-Myb
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10593981
Proper citation: RRID:Addgene_25965 Copy
Genetic Insert: Vinculin tension sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20613844
Comments: Note that this vector lacks the SP6 sequence present in pcDNA3.1. There is a small insertion in the vector sequence, upstream of Vinculin. Plasmid function should not be affected.
Proper citation: RRID:Addgene_26019 Copy
Species: Homo sapiens
Genetic Insert: Cyclin B1
Vector Backbone Description: Backbone Marker:See note below; Backbone Size:4700; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20412769
Comments: The backbone was derived from pECFP-N1. ECFP was replaced with mCherry.
There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation.
Proper citation: RRID:Addgene_26063 Copy
Species: Homo sapiens
Genetic Insert: Cyclin B1
Vector Backbone Description: Backbone Marker:See note below; Backbone Size:4500; Vector Backbone:pVenus-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: pVenus-N1 was derived from pEYFP vector from Clontech.
pVenus-N1 contains no Pst site
Proper citation: RRID:Addgene_26062 Copy
Genetic Insert: Cdk1 FRET Sensor
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20412769
Comments: Original plasmid: pEYFP-C1 from Clontech, CMV promoter has been modified to introduce a T7 sequence and contains the extra sites HindIII, SacI and NcoI.
Domains: YFP variant-PoloBox Domain-flexible linker-Phospho site-CFP variant-FLAG
Proper citation: RRID:Addgene_26064 Copy
Vector Backbone Description: Backbone Size:5076; Vector Backbone:pBMTBX-2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20148414
Proper citation: RRID:Addgene_26073 Copy
Species: Homo sapiens
Genetic Insert: I-kappa-B kinase epsilon K38A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12692549
Proper citation: RRID:Addgene_26202 Copy
Species: Homo sapiens
Genetic Insert: CD11b promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3199; Vector Backbone:pGEM3zf-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7811988
Comments: The cloned CD11b promoter in this construct contains some 5'UTR, including the transcription start site.
Proper citation: RRID:Addgene_26168 Copy
Species: Homo sapiens
Genetic Insert: I-kappa-B kinase epsilon
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12692549
Proper citation: RRID:Addgene_26201 Copy
Vector Backbone Description: Backbone Size:5000; Vector Backbone:modified pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17694064
Comments: This plasmid is to be used ONLY as a negative control for the CMV-d2eGFP plasmids in the associated paper. The multiple cloning site has been destroyed and should not be used for further cloning.
Proper citation: RRID:Addgene_26164 Copy
Species: Mycobacterium marinum + Photorhabdus luminescens
Genetic Insert: G13 promoter + Bacterial luciferase operon
Vector Backbone Description: Backbone Size:4373; Vector Backbone:pMV306hsp; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20520722
Comments: The sequence of the Lux operon is theoretical. As such, there are a few differences between Addgene sequence and full plasmid sequence. These differences do not affect plasmid function.
Qazi, S.N., et al., agr expression precedes escape of internalized Staphylococcus aureus from the host endosome. Infect Immun, 2001. 69(11): p. 7074-82.
Proper citation: RRID:Addgene_26160 Copy
Species: Mycobacterium bovis BCG
Genetic Insert: hsp60 promoter
Vector Backbone Description: Backbone Size:3938; Vector Backbone:pMV306; Vector Types:Mycobacteria integrating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20520722
Comments: There are a few sequence discrepancies between author sequence and Addgene quality control sequence for the hsp promoter. The hsp promoter sequence was taken from the pSMT3 vector and is theoretical sequence. These differences do not affect function.
Garbe, T.R., et al., Transformation of mycobacterial species using hygromycin resistance as selectable marker. Microbiology, 1994. 140 ( Pt 1): p. 133-8.
Proper citation: RRID:Addgene_26155 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php
Proper citation: RRID:Addgene_26094 Copy
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