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Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate CDS module, containing mScarlet, DOI: 10.1038/nmeth.4074.
Proper citation: RRID:Addgene_136967 Copy
Species: Homo sapiens
Genetic Insert: HPV 16 E7 E26G
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV bam neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_13701 Copy
Species: Synthetic
Genetic Insert: XVE
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate CDS module containing the XVE CDS for beta-Estradiole induction; very handy for two constructs on one T-DNA; see DOI: 10.1371/journal.pone.0083043.
Proper citation: RRID:Addgene_136970 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34009392
Comments: For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate C-tag module, containing Venus fused to a flexible linker described here: DOI: 10.1073/pnas.1406446111.
Proper citation: RRID:Addgene_136973 Copy
Species: Synthetic
Genetic Insert: SV40 NLS
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate C-tag module, containing 2 NLS's in sequence
Proper citation: RRID:Addgene_136977 Copy
Species: Synthetic
Genetic Insert: seed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate resistance cassette module, containing the seed coat marker --> DOI:10.1111/j.1365-313X.2009.04060.x
Proper citation: RRID:Addgene_136980 Copy
Species: Synthetic
Genetic Insert: Sa guide RNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate special module, containing the guideRNA backbone. Assembly of the guide is described here: DOI: 10.1007/978-1-4939-7286-9 _27.
Proper citation: RRID:Addgene_136982 Copy
Species: Synthetic
Genetic Insert: seed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate resistance cassette module, containing the seed coat marker --> DOI:10.1111/j.1365-313X.2009.04060.x
Proper citation: RRID:Addgene_136981 Copy
Species: Homo sapiens
Genetic Insert: gRNAs targeting human genome
Vector Backbone Description: Vector Backbone:pRDA_118 (Addgene #133459); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32029722
Proper citation: RRID:Addgene_136986 Copy
Genetic Insert: HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14722277
Proper citation: RRID:Addgene_136985 Copy
Species: Mus musculus
Genetic Insert: gRNAs targeting mouse genome
Vector Backbone Description: Vector Backbone:pRDA_118 (Addgene #133459); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32029722
Proper citation: RRID:Addgene_136987 Copy
Species: Synthetic
Genetic Insert: BlasCyGO
Vector Backbone Description: Vector Backbone:Adapted from pLentiCRISPRv1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32112097
Proper citation: RRID:Addgene_136906 Copy
Species: Synthetic
Genetic Insert: Luc2CyGo
Vector Backbone Description: Vector Backbone:Adapted from pLentiCRISPRv1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32112097
Proper citation: RRID:Addgene_136905 Copy
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pCD-betaG-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This vector contains 2 copies of the APEX2 gene for increased activity.
Proper citation: RRID:Addgene_136991 Copy
Vector Backbone Description: Backbone Marker:Zhao lab; Backbone Size:5300; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31857575
Proper citation: RRID:Addgene_136993 Copy
Vector Backbone Description: Backbone Marker:Zhao lab; Backbone Size:5233; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31857575
Proper citation: RRID:Addgene_136992 Copy
Genetic Insert: EGFP-P4M-SidMx2
Vector Backbone Description: Vector Backbone:pEF1α-IRES-EGFP (IRES-EGFP removed); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_136997 Copy
Species: Synthetic
Genetic Insert: sgAdGO2
Vector Backbone Description: Vector Backbone:Adapted from pLentiCRISPRv1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32112097
Proper citation: RRID:Addgene_136911 Copy
Species: Synthetic
Genetic Insert: sgAdGO
Vector Backbone Description: Vector Backbone:Adapted from pLentiCRISPRv1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32112097
Proper citation: RRID:Addgene_136910 Copy
Genetic Insert: PH-PLC-delta-EGFP
Vector Backbone Description: Vector Backbone:pEF1α-IRES-EGFP (IRES-EGFP removed); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_136998 Copy
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