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Species: Synthetic
Genetic Insert: CytERM
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30648295
Proper citation: RRID:Addgene_98833 Copy
Species: Synthetic
Genetic Insert: CytERM
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27869816
Proper citation: RRID:Addgene_98834 Copy
Species: Synthetic
Genetic Insert: mScarletI-mTurquoise2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28931898
Proper citation: RRID:Addgene_98839 Copy
Species: Synthetic
Genetic Insert: Giantin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_98880 Copy
Genetic Insert: CAN1.y gRNA
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Derived from p426-SNR52p-gRNA.CAN1.Y-SUP4t, provided by George Church. Contains modifications to the gRNA promoter and structural region that incorporate a BstEII and SphI site for cloning purposes.
Proper citation: RRID:Addgene_98814 Copy
Species: Synthetic
Genetic Insert: mts
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/early/2017/07/06/160374 for bioRxiv preprint.
Proper citation: RRID:Addgene_98818 Copy
Species: Other
Genetic Insert: ArchT-GFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV with CaMKII promoter; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21811444
Proper citation: RRID:Addgene_99039 Copy
Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25848771
Comments: The His tag in N'-FtsZ was removed. 6bp were added between the RBS and start codon of FtsZ to decrease the expression level of FstZ-GFP.
Proper citation: RRID:Addgene_98915 Copy
Species: Synthetic
Genetic Insert: Venus KRasCT
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29937389
Comments: The Venus KRasCT fragment was made as a gBlock with HindIII and XbaI, cut and cloned into pcDNA3.1
Proper citation: RRID:Addgene_99016 Copy
Species: Homo sapiens
Genetic Insert: SF2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28323816
Proper citation: RRID:Addgene_99021 Copy
Species: Homo sapiens
Genetic Insert: CCR5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5344; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29678950
Proper citation: RRID:Addgene_98943 Copy
Species: Homo sapiens
Genetic Insert: CXCR4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29678950
Proper citation: RRID:Addgene_98944 Copy
Species: Homo sapiens
Genetic Insert: CCR5
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29678950
Proper citation: RRID:Addgene_98950 Copy
Species: Synthetic
Genetic Insert: Lamp1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_98882 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-mTurquoise2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28931898
Proper citation: RRID:Addgene_98886 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-2A-mTurquoise2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28931898
Proper citation: RRID:Addgene_98885 Copy
Species: Synthetic
Genetic Insert: GCaMP6f tethered to mRuby
Vector Backbone Description: Backbone Marker:Bio Bricks - iGem parts combined by Ben Dodd; Backbone Size:2384; Vector Backbone:BB118; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28808010
Proper citation: RRID:Addgene_98920 Copy
Species: Synthetic
Genetic Insert: GFP-smFP-myc
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Comments: Penn Vector Core PV3661
Proper citation: RRID:Addgene_98927 Copy
Species: Synthetic
Genetic Insert: iGluSnFr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314171
Comments: Penn Vector Core PV2723. Biosensor iGluSnFRa is a single-wavelength glutamate sensor constructed from E. coli GltI and cpGFP. iGluSnFR is bright and photostable, with 4.5 (_F/F)max in vitro, under both 1- and 2-photon illumination.
These iGluSnFR constructs contain a K158R (GFP numbering) mutation that is not referenced in the publication. Depositing lab has confirmed that this mutation is expected and that the plasmids function as described.
** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
Proper citation: RRID:Addgene_98929 Copy
Species: Synthetic
Genetic Insert: Neomycin-T2A-ISce1-ISce1-eGFP
Vector Backbone Description: Backbone Marker:Eric Campeau; Backbone Size:6700; Vector Backbone:pLenti CMV Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28959974
Proper citation: RRID:Addgene_98895 Copy
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