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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pFHMSP-LIC-N
 
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RRID:Addgene_26095 Ampicillin The pFHMSP-LIC N vector is a derivative of the pFastBac HT A vector (Invitrogen). Honeybee melittin signal sequence was introduced before His tag. It is a donor vector for generation of recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector (bacmid) in E. coli host strain, DH10Bac. For use in Bac-to-Bac Baculovirus Expression System in insect cells for secreted protein expression. This vector adds a 26 amino acid N-terminal fusion tag containing 6X His followed by a TEV cleavage site. Insertion of DNA sequence into the cloning/expression region is performed using BD-Biosciences Infusion enzyme mediated by directional recombination between complementary nucleotide DNA sequences at the ends of the insert (PCR product) and Nco1/HindIII linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose. http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php Backbone Marker:Structural Genomics Consortium; Vector Backbone:pFHMSP-LIC-N; Vector Types:Insect Expression, Baculovirus expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:56 0
pBS-elav-2
 
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RRID:Addgene_26131 Smed-elav2 Schmidtea mediterranea Ampicillin PMID:20844018 Backbone Size:3000; Vector Backbone:pBluescript II SK(+); Vector Types:cloning; Bacterial Resistance:Ampicillin 2026-08-29 01:02:56 0
pBS-elav-1
 
Resource Report
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1+ mentions
RRID:Addgene_26130 Smed-elav1 Schmidtea mediterranea Ampicillin PMID:20844018 Backbone Size:3000; Vector Backbone:pBluescript II SK(+); Vector Types:cloning; Bacterial Resistance:Ampicillin 2026-08-29 01:02:56 1
23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U
 
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RRID:Addgene_26191 23S ribosomal RNA fragment (modified) with G2553U mutation Ampicillin PMID:20673833 Backbone Size:2876; Vector Backbone:modified pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin fragment 2508-2580 of E. coli rRNA, with nucleotides 2530-2534 replaced with GAAAC and an rA 3' to nucleotide 2580 plus G2553U mutation 2026-08-29 01:02:59 0
pBMTBX-1
 
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RRID:Addgene_26072 Ampicillin PMID:20148414 Backbone Size:5120; Vector Backbone:pBMTBX-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:55 0
J72008 bacterial strain with plasmid
 
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RRID:Addgene_26187 phi80 integration helper plasmid pInt80-649 Ampicillin PMID:20205762 Backbone Size:0; Vector Backbone:derivative of helper plasmid pAH123; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:02:58 0
pMLLKA-J72039
 
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RRID:Addgene_26184 Med7 aa102-205 for C-terminal tagging Saccharomyces cerevisiae Ampicillin and Kanamycin Note that this plasmid needs to be grown in both Kanamycin and Ampicillin Backbone Size:2852; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-29 01:02:57 0
pMLLCK-J72038
 
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RRID:Addgene_26183 Med21 aa1-132 for N-terminal tagging Saccharomyces cerevisiae Chloramphenicol and Kanamycin Note that this plasmid must be grown in both Chloramphenicol and Kanamycin Backbone Size:2646; Vector Backbone:pMLL-CK; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Chloramphenicol and Kanamycin 2026-08-29 01:02:57 0
pMLLKA-J72040
 
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RRID:Addgene_26185 Med21 aa1-132 for C-terminal tagging Saccharomyces cerevisiae Ampicillin and Kanamycin Note that this plasmid needs to be grown in both Kanamycin and Ampicillin Backbone Size:2852; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin 2026-08-29 01:02:57 0
pBTBXh-4
 
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RRID:Addgene_26080 Tetracycline PMID:20148414 There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function. Backbone Size:4224; Vector Backbone:pBTBXh-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-29 01:02:55 0
pCA-ZtTA
 
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RRID:Addgene_26083 a loxP-flanked β-geo preceding the tetracycline transactivator tTA E. coli Ampicillin PMID:20634890 Backbone Size:5100; Vector Backbone:pCA-HZ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:55 0
pROSA26-ZtTA
 
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RRID:Addgene_26082 a loxP-flanked β-geo preceding the tetracycline transactivator tTA E. coli Ampicillin PMID:20634890 6bp deletion in Addgene sequence compared to author sequence. The discrepancy is in the vector before the BGH terminator and is not likely to affect function. Backbone Size:12000; Vector Backbone:pROSA26-PA targeting vector; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin 2026-08-29 01:02:55 0
pRK-ATF4 1-275
 
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1+ mentions
RRID:Addgene_26118 ATF4 Homo sapiens Ampicillin PMID:19164757 Backbone Marker:Dr. Hongbin Shu; Vector Backbone:pRK5-A20 (TNFAIP3); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin aa's 276-351 have been deleted 2026-08-29 01:02:56 1
pRK-ATF4
 
Resource Report
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10+ mentions
RRID:Addgene_26114 ATF4 Homo sapiens Ampicillin PMID:19164757 Backbone Marker:Dr. Hongbin Shu; Vector Backbone:pRK5-A20 (TNFAIP3); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:56 19
pNIC-CH
 
Resource Report
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1+ mentions
RRID:Addgene_26117 Kanamycin pET expression vector with C-terminal His6 tag. Includes sites for LIC cloning, and a “stuffer” fragment that includes the SacB gene, allowing negative selection on 5% sucrose. GenBank accession number: EF199843 Primers for LIC cloning: Add the following 5’ extensions to the PCR primers: Upstream: TTAAGAAGGAGATATACTATG (ATG-initiation codon) Downstream: AATGGTGGTGATGATGGTGCGC The purified PCR fragments are treated with T4 DNA polymerase and dGTP, then annealed to the treated vector. See supplemental document for more information. Backbone Marker:SGC Oxford; Backbone Size:7218; Vector Backbone:pNIC-CH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:02:56 7
pBTBXh-2
 
Resource Report
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RRID:Addgene_26078 Kanamycin PMID:20148414 There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function. Backbone Size:3845; Vector Backbone:pBTBXh-2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-29 01:02:55 0
pBTBXh-1
 
Resource Report
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RRID:Addgene_26077 Ampicillin PMID:20148414 There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function. Backbone Size:3889; Vector Backbone:pBTBXh-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:02:55 0
pBTBXh-3
 
Resource Report
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RRID:Addgene_26079 Chloramphenicol PMID:20148414 There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function. Backbone Size:3691; Vector Backbone:pBTBXh-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-29 01:02:55 0
PGL3-NOXA-N1
 
Resource Report
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1+ mentions
RRID:Addgene_26112 NOXA N1 Homo sapiens Ampicillin PMID:19164757 Backbone Size:5000; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-29 01:02:56 3
pBMTBX-3
 
Resource Report
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RRID:Addgene_26074 Chloramphenicol PMID:20148414 Backbone Size:4922; Vector Backbone:pBMTBX-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-29 01:02:55 0

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