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Vector Backbone Description: Backbone Size:5076; Vector Backbone:pBMTBX-2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20148414
Proper citation: RRID:Addgene_26073 Copy
Species: Homo sapiens
Genetic Insert: HRAS-V12
Vector Backbone Description: Backbone Marker:Verma lab; Backbone Size:8753; Vector Backbone:pTomo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19122659
Comments: From the paper: To construct pTomo H-RasV12, we amplified Flag-tagged H-RasV12 from pGEM H-RasV12 by PCR and inserted it into the BamHI site of the pTomo vector.
Proper citation: RRID:Addgene_26292 Copy
Species: Homo sapiens
Genetic Insert: pSUPER IKK beta
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12692549
Proper citation: RRID:Addgene_26209 Copy
Species: Homo sapiens
Genetic Insert: I-kappa-B kinase epsilon K38A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12692549
Proper citation: RRID:Addgene_26202 Copy
Genetic Insert: no insert
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7072; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: A cDNA or miRNA can be inserted downstream of the CMV promoter. There is an IRES with the red fluorescent protein downstream so the transduced cells will be red.
Proper citation: RRID:Addgene_26287 Copy
Species: Homo sapiens
Genetic Insert: I-kappa-B kinase epsilon
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12692549
Proper citation: RRID:Addgene_26201 Copy
Species: Mus musculus
Genetic Insert: mCD8::GFP
Vector Backbone Description: Backbone Size:8023; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26219 Copy
Vector Backbone Description: Backbone Size:7831; Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26213 Copy
Species: Mus musculus
Genetic Insert: mCD8::GFP
Vector Backbone Description: Backbone Size:10785; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26215 Copy
Species: Homo sapiens
Genetic Insert: pSUPER TBK1
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12692549
Proper citation: RRID:Addgene_26210 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4569; Vector Backbone:pBluescript II KS+; Vector Types:Trypanosome in-situ tagging vector; Bacterial Resistance:Ampicillin
Comments: Modifications giving different epitope tags and/or selectable markers made by members of the Cross lab to plasmids based on Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.
A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120.
Proper citation: RRID:Addgene_26298 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3868; Vector Backbone:pBluescript II KS+; Vector Types:Trypanosome in-situ tagging vector; Bacterial Resistance:Ampicillin
Comments: Modifications giving different epitope tags and/or selectable markers made by members of the Cross lab to plasmids based on Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.
A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120.
Proper citation: RRID:Addgene_26297 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3952; Vector Backbone:pBluescript II KS+; Vector Types:Trypanosome in-situ tagging vector; Bacterial Resistance:Ampicillin
Comments: Modifications giving different epitope tags and/or selectable markers made by members of the Cross lab to plasmids based on Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T.
A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120.
Proper citation: RRID:Addgene_26296 Copy
Species: Homo sapiens
Genetic Insert: hsa-mir-142
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26309 Copy
Vector Backbone Description: Backbone Size:7401; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for puromycin selection of transgenes in C. elegans.
This vector is identical to pBCN21-R4R3, but a 8xC183_minPmyo-2::mCherry::unc-54_3'UTR cassette was inserted in the backbone as an additional visual marker.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26302 Copy
Vector Backbone Description: Backbone Size:8090; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for puromycin selection of transgenes in Caenorhabditis species other than C. elegan (works also in C. elegans).
This vector is the same as pBCN22-R4R3 but with a different Pmyo-2::mCherry::unc-54_3'UTR construct that gives good fluoresence expression in the pharynx of C. elegans as well as other species, e.g. C. briggsae, C. remanei and C. brenneri.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21473015
Proper citation: RRID:Addgene_26265 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ZIP- Gal4DBD
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21473015
Comments: Luan et al. Neuron. 2006 Nov 9. 52(3):425-36.
Proper citation: RRID:Addgene_26264 Copy
Species: Neurospora crassa
Genetic Insert: QF
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21473015
Proper citation: RRID:Addgene_26267 Copy
Species: Trypanosoma brucei
Genetic Insert: VSG117 (Lister 427-4) cDNA
Vector Backbone Description: Backbone Size:4916; Vector Backbone:pLEW82; Vector Types:Trypanosome inducible expression vector; Bacterial Resistance:Ampicillin
Comments: 2002: Mutational analysis of the variant surface glycoprotein GPI-anchor signal sequence in Trypanosoma brucei. Boehme U & Cross GAM. J Cell Sci 115:805-16
Proper citation: RRID:Addgene_26300 Copy
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