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Species: Synthetic
Genetic Insert: iGluSnFr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314171
Comments: Penn Vector Core PV2914. Biosensor iGluSnFRa is a single-wavelength glutamate sensor constructed from E. coli GltI and cpGFP. iGluSnFR is bright and photostable, with 4.5 (_F/F)max in vitro, under both 1- and 2-photon illumination.
These iGluSnFR constructs contain a K158R (GFP numbering) mutation that is not referenced in the publication. Depositing lab has confirmed that this mutation is expected and that the plasmids function as described.
** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
Proper citation: RRID:Addgene_98930 Copy
Species: Synthetic
Genetic Insert: Neomycin-T2A-mCherry-GFP
Vector Backbone Description: Backbone Size:3050; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28959974
Proper citation: RRID:Addgene_98896 Copy
Species: Synthetic
Genetic Insert: iGluSnFr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314171
Comments: Penn Vector Core PV2724. Biosensor iGluSnFRa is a single-wavelength glutamate sensor constructed from E. coli GltI and cpGFP. iGluSnFR is bright and photostable, with 4.5 (_F/F)max in vitro, under both 1- and 2-photon illumination.
These iGluSnFR constructs contain a K158R (GFP numbering) mutation that is not referenced in the publication. Depositing lab has confirmed that this mutation is expected and that the plasmids function as described.
** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
Proper citation: RRID:Addgene_98931 Copy
Species: Other
Genetic Insert: Dimeric MS2-coat binding protein
Vector Backbone Description: Backbone Size:10000; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28562589
Proper citation: RRID:Addgene_99151 Copy
Species: Other
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99131 Copy
Species: Other
Genetic Insert: NLS-mRuby2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99130 Copy
Species: Other
Genetic Insert: fDIO-mNeonGreen
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99133 Copy
Species: Other
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99135 Copy
Genetic Insert: humanized Cas9
Vector Backbone Description: Vector Backbone:pCI; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29150011
Proper citation: RRID:Addgene_99138 Copy
Genetic Insert: Control gRNA
Vector Backbone Description: Vector Backbone:pCESAx; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29150011
Proper citation: RRID:Addgene_99140 Copy
Genetic Insert: Cas9-GFP
Vector Backbone Description: Vector Backbone:pCI; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29150011
Proper citation: RRID:Addgene_99141 Copy
Species: Homo sapiens
Genetic Insert: UPF1
Vector Backbone Description: Backbone Marker:Addgene plasmid #41393; Backbone Size:7600; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28669802
Comments: The lentiviral pCW57-FLAG-UPF1wt plasmid was used as a template to introduce the S124A/N138A/ T139A mutations with PCR primers containing mismatched nucleotides that altered the original codons to GCT (Alanine).
Proper citation: RRID:Addgene_99144 Copy
Species: Mus musculus
Genetic Insert: cAMPr
Vector Backbone Description: Backbone Marker:Michael Kyba; Backbone Size:2660; Vector Backbone:p2Lox; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29511120
Proper citation: RRID:Addgene_99143 Copy
Species: Synthetic
Genetic Insert: 47xCAG 12xMS2 hairpins
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8700; Vector Backbone:pHR-TRE3G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28562589
Proper citation: RRID:Addgene_99148 Copy
Species: Synthetic
Genetic Insert: 29xGGGGCC 12xMS2 hairpins
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8700; Vector Backbone:pHR-TRE3G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28562589
Comments: 29x GGGGCC cannot be confirmed by sequencing due to high GC content. Addgene was able to verify at least 16x repeats. Instability may be observed in the repeat region so it is recommended that multiple DNA preparations be screened.
Proper citation: RRID:Addgene_99149 Copy
Species: Homo sapiens
Genetic Insert: ATIC
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18388293
Proper citation: RRID:Addgene_99110 Copy
Species: Chlamydomonas noctigama
Genetic Insert: ChrimsonR-tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson (original AAV-CAG); Backbone Size:5088; Vector Backbone:AAV-CaMKII; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
In addition, the published CaMKII promoter contains 10 basepair differences vs. the wild-type, and that persists here.
Proper citation: RRID:Addgene_99231 Copy
Species: Other
Genetic Insert: DIO-mTurquoise2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99115 Copy
Species: Schmidtea mediterranea
Genetic Insert: Cyp1A1 (also known as h.49.4f) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TTGAAATTGGAGCGAAAATTAAA, R-GGGGAAATTCTTGAACCCTTC
Note: The Cyp1A1 insert contains several mismatches compared to AY067980.1. The depositor has confirmed this does not impact plasmid function.
Proper citation: RRID:Addgene_99081 Copy
Species: Other
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99122 Copy
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