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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pXL001
 
Resource Report
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1+ mentions
RRID:Addgene_26122 tTR-KRAB trans-activator - KRAB Ampicillin PMID:22645348 Backbone Size:7569; Vector Backbone:pSin-EF2-Nanog; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 09:51:34 3
CMV::ratSyGCaMP2
 
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10+ mentions
RRID:Addgene_26124 Synaptophysin GCaMP2 Rattus norvegicus Kanamycin PMID:19898484 ORF frame 3 (624-2915): Rat SyGCaMP2. Our sequence with CMV-F shows a mutation S199T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP. Backbone Size:3957; Vector Backbone:pEGFP-N1 (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:51:34 10
pCA-mTmG
 
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1+ mentions
RRID:Addgene_26123 Precursor mT/mG Aquorea victoria Ampicillin Backbone Size:5000; Vector Backbone:pCA-HZ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:51:34 3
pSVE Grb2 myc 49L
 
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1+ mentions
RRID:Addgene_26085 Grb2 P49L Homo sapiens Ampicillin PMID:8479536 mutagenesis performed using oligo GGA AAA GAC GGC TTC ATT TTA AAG AAC TAC ATA GAA ATG, which creates a new DraI site and mutates codon 49 from P to L. Backbone Size:0; Vector Backbone:pSVE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin P49L 2026-09-01 09:51:33 1
pET28-MHL
 
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10+ mentions
RRID:Addgene_26096 Kanamycin The pET28-MHL vector (GenBank accession EF456735) was derived from expression plasmid pET28a-LIC (SGC). It is used for T7 promoter driven expression of recombinant proteins with the addition of an 18 amino acid N-terminal fusion tag containing 6X His followed by a TEV cleavage site. The GSS residues after the Met start site were removed to reduce N-terminal gluconoylation via preventing N-terminal Met excision. Two stop codons are included in the vector at the C-terminal cloning site. Insertion of DNA sequence into the cloning/expression region is performed using BD-Biosciences Infusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose. http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php Backbone Marker:Structural Genomics Consortium; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:51:33 21
pBS-elav-1
 
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1+ mentions
RRID:Addgene_26130 Smed-elav1 Schmidtea mediterranea Ampicillin PMID:20844018 Backbone Size:3000; Vector Backbone:pBluescript II SK(+); Vector Types:cloning; Bacterial Resistance:Ampicillin 2026-09-01 09:51:34 1
pRK-ATF4 1-275
 
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1+ mentions
RRID:Addgene_26118 ATF4 Homo sapiens Ampicillin PMID:19164757 Backbone Marker:Dr. Hongbin Shu; Vector Backbone:pRK5-A20 (TNFAIP3); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin aa's 276-351 have been deleted 2026-09-01 09:51:34 1
pRK-ATF4
 
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10+ mentions
RRID:Addgene_26114 ATF4 Homo sapiens Ampicillin PMID:19164757 Backbone Marker:Dr. Hongbin Shu; Vector Backbone:pRK5-A20 (TNFAIP3); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:51:34 19
pNIC-CH
 
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1+ mentions
RRID:Addgene_26117 Kanamycin pET expression vector with C-terminal His6 tag. Includes sites for LIC cloning, and a “stuffer” fragment that includes the SacB gene, allowing negative selection on 5% sucrose. GenBank accession number: EF199843 Primers for LIC cloning: Add the following 5’ extensions to the PCR primers: Upstream: TTAAGAAGGAGATATACTATG (ATG-initiation codon) Downstream: AATGGTGGTGATGATGGTGCGC The purified PCR fragments are treated with T4 DNA polymerase and dGTP, then annealed to the treated vector. See supplemental document for more information. Backbone Marker:SGC Oxford; Backbone Size:7218; Vector Backbone:pNIC-CH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:51:34 7
pAAV-EF1a-FLEX-GT
 
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10+ mentions
RRID:Addgene_26198 Enhanced green fluorescent protein, TVA Mus musculus Ampicillin PMID:21115815 Backbone Marker:Karl Deisseroth lab; Backbone Size:5300; Vector Backbone:pAAV-double floxed-EYFP-WPRE-pA; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin TVA mammalian codon optimized 2026-09-01 09:51:36 20
PGL3-NOXA-N1
 
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1+ mentions
RRID:Addgene_26112 NOXA N1 Homo sapiens Ampicillin PMID:19164757 Backbone Size:5000; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-09-01 09:51:34 3
pAAV-FLEX-hGTB
 
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1+ mentions
RRID:Addgene_26196 Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein Homo sapiens Kanamycin PMID:21115815 Backbone Marker:Mr. Gene; Backbone Size:3300; Vector Backbone:pAAV; Vector Types:AAV, Cre/Lox, Adeno-associated virus; Bacterial Resistance:Kanamycin F2A has 2A cleavage site deleted, producing htTA-eGFP fusion. tTA, TVA, and B19G are all mammalian codon optimized 2026-09-01 09:51:36 3
pTomo-Ras
 
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1+ mentions
RRID:Addgene_26292 HRAS-V12 Homo sapiens Ampicillin PMID:19122659 From the paper: To construct pTomo H-RasV12, we amplified Flag-tagged H-RasV12 from pGEM H-RasV12 by PCR and inserted it into the BamHI site of the pTomo vector. Backbone Marker:Verma lab; Backbone Size:8753; Vector Backbone:pTomo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin G12V 2026-09-01 09:51:37 3
pSUPER IKKbeta
 
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1+ mentions
RRID:Addgene_26209 pSUPER IKK beta Homo sapiens Ampicillin PMID:12692549 Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:RNAi; Bacterial Resistance:Ampicillin 2026-09-01 09:51:36 1
pJFRC-MUH
 
Resource Report
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10+ mentions
RRID:Addgene_26213 Ampicillin PMID:20697123 Backbone Size:7831; Vector Backbone:pBDP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:51:36 27
pSUPER TBK1
 
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1+ mentions
RRID:Addgene_26210 pSUPER TBK1 Homo sapiens Ampicillin PMID:12692549 Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:RNAi; Bacterial Resistance:Ampicillin 2026-09-01 09:51:36 3
pMOTag2H
 
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1+ mentions
RRID:Addgene_26296 Ampicillin Modifications giving different epitope tags and/or selectable markers made by members of the Cross lab to plasmids based on Oberholzer,M., Morand,S., Kunz,S., and Seebeck,T. A vector series for rapid PCR-mediated C-terminal in-situ tagging of T. brucei genes. Mol. Biochem. Parasitol. 145, 2005, 117-120. Backbone Marker:Stratagene; Backbone Size:3952; Vector Backbone:pBluescript II KS+; Vector Types:Trypanosome in-situ tagging vector; Bacterial Resistance:Ampicillin 2026-09-01 09:51:37 1
pXN-FBLWLF-GAL4DBD
 
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1+ mentions
RRID:Addgene_26264 ZIP- Gal4DBD Saccharomyces cerevisiae Ampicillin PMID:21473015 Luan et al. Neuron. 2006 Nov 9. 52(3):425-36. Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin 2026-09-01 09:51:37 1
pXN-FBLWLF-GAL4AD
 
Resource Report
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1+ mentions
RRID:Addgene_26263 Gal4AD-Zip+ Saccharomyces cerevisiae Ampicillin PMID:21473015 Luan et al. Neuron. 2006 Nov 9. 52(3):425-36. Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin 2026-09-01 09:51:37 1
pGL2- Empty control -SV40-Luc
 
Resource Report
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1+ mentions
RRID:Addgene_26280 pGL2-Control plasmid Ampicillin PMID:20442331 Backbone kindly provided by Dr. Ron Mckay and Dr.Paul Tesar (Tesar et al. Nature 2007) Backbone Size:6000; Vector Backbone:pGL2-SV40-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-09-01 09:51:37 6

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