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Species: Mus musculus
Genetic Insert: TCR beta cDNA clone HSV2.3
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:3947; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:11940116
Comments: This plasmid contains the TCR beta chain cDNA from the HSV2.3 clone and was used to create the transgenic construct p3A9cbetaTCR.HSV2.3
(Addgene plasmid #69594).
Proper citation: RRID:Addgene_74077 Copy
Species: Mus musculus
Genetic Insert: EKLF
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4969; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9707565
Comments: Mutations G299S, and T305S have no functional consequences.
Proper citation: RRID:Addgene_67836 Copy
Species: Mus musculus
Genetic Insert: EKLF
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11287616
Comments: Mutations G299S, T305S have no functional consequences.
Proper citation: RRID:Addgene_67837 Copy
Species: Mus musculus
Genetic Insert: EKLF
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4076; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25197097
Comments: Entire EKLF open reading frame is defined as nucleotides 71-1215 and described in PMID 7682653. Mutations G299S and T305S have no functional consequences.
Proper citation: RRID:Addgene_67833 Copy
Species: Mus musculus
Genetic Insert: AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC; Vector Types:Mammalian Expression, Mouse Targeting, AAV, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26094607
Comments: this plasmid is modified from the pRIME system. See:
Stegmeier, F., Hu, G., Rickles, R.J., Hannon, G.J., and Elledge, S.J. (2005). A
lentiviral microRNA-based system for single-copy polymerase II-regulated
RNA interference in mammalian cells. Proc. Natl. Acad. Sci. USA 102,
13212–13217
We used The pPRIME-CMV-dsRed-FF3 vector, a gift from Stephen Elledge (Addgene plasmid 11664), as the building block to make the AAV-hsyn-flex-dsRed-miR30-vgat2.shRNA plasmid.
transgene expression is Cre-dependent; contains flex switch
All the enzymes are unique cutters, except EcoRI and XhoI, the insert dsRed-shRNA can be retrieved using AscI and NheI, giving 1.2kb and 4.8kb bands.
The insert was read using primers in the dsred sequence:
dsred 410: CGTAATGCAGAAGAAGACTATG
dsred 530R: CCATGTAGATGGACTTGAACTC
Proper citation: RRID:Addgene_67845 Copy
Species: Mus musculus
Genetic Insert: Cav3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:peGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10385524
Proper citation: RRID:Addgene_67886 Copy
Species: Mus musculus
Genetic Insert: Vinculin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25065757
Proper citation: RRID:Addgene_67935 Copy
Species: Mus musculus
Genetic Insert: YC3.60
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26435194
Proper citation: RRID:Addgene_67899 Copy
Species: Mus musculus
Genetic Insert: ZFP809
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19270682
Proper citation: RRID:Addgene_67788 Copy
Species: Mus musculus
Genetic Insert: nesprin-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcdna 3.1 -; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26745407
Proper citation: RRID:Addgene_68128 Copy
Species: Mus musculus
Genetic Insert: mouse nesprin mini 2G
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcdna 3.1 +; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26745407
Proper citation: RRID:Addgene_68127 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68029 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4F
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68033 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68032 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68031 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68030 Copy
Species: Mus musculus
Genetic Insert: Plexin-B3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68039 Copy
Species: Mus musculus
Genetic Insert: Plexin-B2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68038 Copy
Species: Mus musculus
Genetic Insert: Cav3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15689493
Comments: This has been made by cloning Cav3P104L from HA tagged Cav3P104L construct as described in Carozzi et al 2002
Proper citation: RRID:Addgene_68404 Copy
Species: Mus musculus
Genetic Insert: IDAX-DBM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6174; Vector Backbone:pEF1a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23563267
Proper citation: RRID:Addgene_68554 Copy
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