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Species: Synthetic
Genetic Insert: DiySpike2d2
Vector Backbone Description: Backbone Marker:Joachim Messing; Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32518404
Proper citation: RRID:Addgene_136956 Copy
Genetic Insert: Firefly luciferase and TurboRFP
Vector Backbone Description: Vector Backbone:pNL4-3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30220977
Proper citation: RRID:Addgene_136959 Copy
Species: Synthetic
Genetic Insert: DiySpike2d4
Vector Backbone Description: Backbone Marker:Joachim Messing; Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32518404
Proper citation: RRID:Addgene_136958 Copy
Species: Synthetic
Genetic Insert: (GGS)2G
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137097 Copy
Species: Synthetic
Genetic Insert: (GGS)4GSG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137099 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP-GLO^SEC^3xFlag + ccdB
Vector Backbone Description: Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_137018 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans GLO-optimized GFP
Vector Backbone Description: Backbone Size:2540; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR, SapTrap donor for cloning repair templates for C. elegans; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137021 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans GLO-optimized Halo Tag
Vector Backbone Description: Backbone Size:2540; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR, SapTrap donor for cloning repair templates for C. elegans; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_137023 Copy
Species: Homo sapiens
Genetic Insert: hTid Short
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV bam neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9683573
Proper citation: RRID:Addgene_13709 Copy
Species: Homo sapiens
Genetic Insert: NIMA-like kinase 10
Vector Backbone Description: Backbone Size:8454; Vector Backbone:pLRC1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31959991
Proper citation: RRID:Addgene_137030 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAC66700.1
Vector Backbone Description: Vector Backbone:pRSET-TEV-12His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7 promoter to express in E. coli the full-length, wild-type protein containing a C-terminal TEV-protease-cleavable His12-tag. The protein sequence includes its N-terminal membrane-targeting signal peptide sequence. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli.
Proper citation: RRID:Addgene_137039 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAC66635.2
Vector Backbone Description: Vector Backbone:pRSET-TEV-12His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7 promoter to express in E. coli the full-length, wild-type protein containing a C-terminal TEV-protease-cleavable His12-tag. The protein sequence includes its N-terminal membrane-targeting signal peptide sequence. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli.
Proper citation: RRID:Addgene_137038 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAC66790.1
Vector Backbone Description: Vector Backbone:pRSET-TEV-12His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7 promoter to express in E. coli the full-length, wild-type protein containing a C-terminal TEV-protease-cleavable His12-tag. The protein sequence includes its N-terminal membrane-targeting signal peptide sequence. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli.
Proper citation: RRID:Addgene_137042 Copy
Species: Mus musculus
Genetic Insert: Gfap
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:pDTA-5; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: A targeting vector to knock-in T2A-td-sfGFP into mouse Gfap protein isoform 2 (also known as Gfap-alpha). This is the most abundant isoform in the mouse brain. Of note, the Gfap protein transcript variant 2 transcript level is high in the SVZ, an adult neural stem cell niche. See Kamphuis et al., 2012 (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0042823).
Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Proper citation: RRID:Addgene_137044 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAC66635.2
Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli.
Proper citation: RRID:Addgene_137046 Copy
Genetic Insert: blasticidin-S deaminase
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Leishmania expression gateway vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137049 Copy
Species: Other
Genetic Insert: puromycin N-acetyl-transferase
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Leishmania expression gateway vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137050 Copy
Vector Backbone Description: Vector Backbone:GOI flox pDONR221; Vector Types:Leishmania floxed gene expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30980305
Proper citation: RRID:Addgene_137055 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA4-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: SSA4 promoter and SSA4 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1371 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAF07416.1
Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli. RevA is a lipoprotein. RevA is not expected to be lipidated in this construct. Following TEV cleavage, RevA lacks 5 residues (CKAYV) at the N-terminus of the mature protein.
Proper citation: RRID:Addgene_137064 Copy
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