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Species: E. coli
Genetic Insert: LexA
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21473015
Proper citation: RRID:Addgene_26266 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4AD-Zip+
Vector Backbone Description: Backbone Size:8757; Vector Backbone:pXN-FBLWLF; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21473015
Comments: Luan et al. Neuron. 2006 Nov 9. 52(3):425-36.
Proper citation: RRID:Addgene_26263 Copy
Genetic Insert: pGL2-Control plasmid
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pGL2-SV40-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20442331
Comments: Backbone kindly provided by Dr. Ron Mckay and Dr.Paul Tesar (Tesar et al. Nature 2007)
Proper citation: RRID:Addgene_26280 Copy
Species: Homo sapiens
Genetic Insert: hsa-mir-193b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26318 Copy
Species: Homo sapiens
Genetic Insert: hsa-mir-214
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26313 Copy
Species: Homo sapiens
Genetic Insert: hsa-mir-205
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26312 Copy
Species: Homo sapiens
Genetic Insert: hsa-mir-150
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26310 Copy
Species: Mus musculus
Genetic Insert: p21 shRNA
Vector Backbone Description: Backbone Size:7560; Vector Backbone:pSicoR; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19898493
Comments: The shRNA is directed against the mouse p21 sequence 5'- gagaacggtggaactttga -3'.
Proper citation: RRID:Addgene_26271 Copy
Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Size:8050; Vector Backbone:pBRYCAG-floxStop dsRED-IresPuro; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20442331
Proper citation: RRID:Addgene_26274 Copy
Species: E. coli
Genetic Insert: LexA
Vector Backbone Description: Backbone Size:7963; Vector Backbone:pBPHLWL; Vector Types:Bacterial Expression, Insect Expression, Drosophila transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21473015
Proper citation: RRID:Addgene_26258 Copy
Species: synthetic
Genetic Insert: mRFPmars
Vector Backbone Description: Backbone Marker:Martin Fraunholz; Backbone Size:6500; Vector Backbone:pCerulean; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20708040
Comments: Antibiotic selection: Ampicillin in E. coli, Chloramphenicol in S. aureus
Proper citation: RRID:Addgene_26252 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4::VP16
Vector Backbone Description: Backbone Size:8770; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26228 Copy
Species: Drosophila melanogaster
Genetic Insert: myr::GFP
Vector Backbone Description: Backbone Size:8033; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26224 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:9223; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26227 Copy
Species: Drosophila melanogaster
Genetic Insert: myr::GFP
Vector Backbone Description: Backbone Size:7904; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26222 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: nlsLexA::GADfl
Vector Backbone Description: Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26232 Copy
Species: Mus musculus
Genetic Insert: Enhanced green fluorescent protein, TVA
Vector Backbone Description: Backbone Marker:Karl Deisseroth lab; Backbone Size:5300; Vector Backbone:pAAV-double floxed-EYFP-WPRE-pA; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21115815
Proper citation: RRID:Addgene_26198 Copy
Species: Homo sapiens
Genetic Insert: p65AD-Zip
Vector Backbone Description: Backbone Size:8731; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26234 Copy
Species: Homo sapiens
Genetic Insert: nlsLexA::p65
Vector Backbone Description: Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26230 Copy
Species: Homo sapiens
Genetic Insert: Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
Vector Backbone Description: Backbone Marker:Mr. Gene; Backbone Size:3300; Vector Backbone:pAAV; Vector Types:AAV, Cre/Lox, Adeno-associated virus; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21115815
Proper citation: RRID:Addgene_26196 Copy
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