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Species: Synthetic
Genetic Insert: N-BLa 1.1 fusion protein
Vector Backbone Description: Backbone Size:4083; Vector Backbone:pACYC184 (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28266525
Comments: TMD: Integrin alphaV mut GP, contains ApaI restriction site
Proper citation: RRID:Addgene_88994 Copy
Species: E.coli
Genetic Insert: csgB
Vector Backbone Description: Backbone Size:2273; Vector Backbone:pZa; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28388843
Proper citation: RRID:Addgene_89580 Copy
Species: B. zoohelcum ATCC 43767
Genetic Insert: Cas13b
Vector Backbone Description: Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28065598
Comments: Benchling sequence link: https://benchling.com/s/seq-gyNy5vRceFfoBk3kqStl
Proper citation: RRID:Addgene_89901 Copy
Species: P. buccae ATCC 33574
Genetic Insert: Cas13b
Vector Backbone Description: Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28065598
Comments: Benchling sequence link: https://benchling.com/s/hqikFD6s
Proper citation: RRID:Addgene_89906 Copy
Species: S. pyogenes
Genetic Insert: Cas9HF1
Vector Backbone Description: Vector Backbone:pCas9-CR4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28915012
Comments: This Cas9 has been modified to contain the high-fidelity mutations for SpCas9-HF1 from Kleinstiver et al 2016
Proper citation: RRID:Addgene_89961 Copy
Species: S. pyogenes
Genetic Insert: Cas9HF1
Vector Backbone Description: Vector Backbone:pEM-Cas9HF1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28915012
Proper citation: RRID:Addgene_89962 Copy
Species: Synthetic
Genetic Insert: Invertase followed by αMFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84972 Copy
Species: Synthetic
Genetic Insert: Red fluorescent protein
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84977 Copy
Species: Synthetic
Genetic Insert: Inulinase followed by αMFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84971 Copy
Species: Synthetic
Genetic Insert: Serum albumin followed by αMFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84970 Copy
Species: Synthetic
Genetic Insert: Enolase 1 promoter
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84962 Copy
Species: Synthetic
Genetic Insert: α-mating factor secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84964 Copy
Species: Synthetic
Genetic Insert: Triose phosphate isomerase 1 promoter
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84963 Copy
Species: Synthetic
Genetic Insert: α-mating factor no EAEA secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84965 Copy
Species: Synthetic
Genetic Insert: α-Amylase followed by MFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84968 Copy
Species: Synthetic
Genetic Insert: Glucoamylase followed by αMFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84969 Copy
Species: Synthetic
Genetic Insert: Pichia autonomously replicating sequence
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MevTaco-MKco-PMKco
Vector Backbone Description: Vector Backbone:pBbA5c; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26052683
Proper citation: RRID:Addgene_84985 Copy
Species: Synthetic
Genetic Insert: Alcohol oxidase 1 terminator
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84988 Copy
Species: Synthetic
Genetic Insert: BxbI recognition site
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84989 Copy
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