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Species: Caenorhabditis elegans
Genetic Insert: MiRP K+ channel accessory Subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17604313
Comments: This plasmid contains an N214K mutation compared to the reference sequence NP_495263.2 that is not of concern for function.
Proper citation: RRID:Addgene_113059 Copy
Species: Caenorhabditis elegans
Genetic Insert: lis-1
Vector Backbone Description: Backbone Marker:L4440 is available at Addgene; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: Full length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11336 Copy
Species: Caenorhabditis elegans
Genetic Insert: Frataxin homologue
Vector Backbone Description: Backbone Size:2676; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17215485
Proper citation: RRID:Addgene_15401 Copy
Species: Caenorhabditis elegans
Genetic Insert: Frataxin homologue
Vector Backbone Description: Backbone Size:2676; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17215485
Proper citation: RRID:Addgene_15400 Copy
Species: Caenorhabditis elegans
Genetic Insert: LoxP-SEC-LoxP
Vector Backbone Description: Backbone Marker:Addgene #42250; Backbone Size:1857; Vector Backbone:DR274; Vector Types:Worm Expression, Cre/Lox, RMCE; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154108 Copy
Species: Caenorhabditis elegans
Genetic Insert: LoxP-SEC-LoxP
Vector Backbone Description: Backbone Marker:Addgene #42250; Backbone Size:1857; Vector Backbone:DR274; Vector Types:Worm Expression, Cre/Lox, RMCE; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154109 Copy
Species: Caenorhabditis elegans
Genetic Insert: lexO 5X::∆pes-10::GFP-C1::tbb-2 3'
Vector Backbone Description: Backbone Marker:Addgene #153083; Backbone Size:7855; Vector Backbone:pLF3FShC; Vector Types:Worm Expression, RMCE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154115 Copy
Species: Caenorhabditis elegans
Genetic Insert: tetO 7X::∆pes-10::GFP-C1::act-4 3'
Vector Backbone Description: Backbone Marker:Addgene #153083; Backbone Size:7855; Vector Backbone:pLF3FShC; Vector Types:Worm Expression, RMCE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154116 Copy
Species: Caenorhabditis elegans
Genetic Insert: loxP::SEC::loxP::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
Vector Backbone Description: Backbone Marker:Addgene #44486; Backbone Size:3173; Vector Backbone:pCFJ1272; Vector Types:miniMos; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154110 Copy
Species: Caenorhabditis elegans
Genetic Insert: loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
Vector Backbone Description: Backbone Marker:Addgene #44486; Backbone Size:3173; Vector Backbone:pCFJ1272; Vector Types:miniMos; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154111 Copy
Species: Caenorhabditis elegans
Genetic Insert: UAS 11X::∆pes-10::GFP-C1::tbb-2 3'
Vector Backbone Description: Backbone Marker:Addgene #153083; Backbone Size:7855; Vector Backbone:pLF3FShC; Vector Types:Worm Expression, RMCE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154113 Copy
Species: Caenorhabditis elegans
Genetic Insert: mec-4p::lexA-L-QFAD::act-4 '3
Vector Backbone Description: Backbone Marker:Addgene #153083; Backbone Size:7855; Vector Backbone:pLF3FShC; Vector Types:Worm Expression, RMCE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154121 Copy
Species: Caenorhabditis elegans
Genetic Insert: mec-4p::tetR-L-QFAD::act-4 '3
Vector Backbone Description: Backbone Marker:Addgene #153083; Backbone Size:7855; Vector Backbone:pLF3FShC; Vector Types:Worm Expression, RMCE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154123 Copy
Species: Caenorhabditis elegans
Genetic Insert: mec-4p::QF2::act-4 '3
Vector Backbone Description: Backbone Marker:Addgene #153083; Backbone Size:7855; Vector Backbone:pLF3FShC; Vector Types:Worm Expression, RMCE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32513816
Proper citation: RRID:Addgene_154120 Copy
Species: Caenorhabditis elegans
Genetic Insert: GluCl beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12648766
Comments: The lab has created an improved version of this plasmid, which can be found at http://www.addgene.org/47542/ .
Proper citation: RRID:Addgene_15105 Copy
Species: Caenorhabditis elegans
Genetic Insert: GluCl alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12648766
Comments: The lab has created an improved version of this plasmid, which can be found at http://www.addgene.org/47387/ .
Proper citation: RRID:Addgene_15104 Copy
Species: Caenorhabditis elegans
Genetic Insert: wrmScarlet11 x3 tandem repeats
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2626; Vector Backbone:pUC57-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693628
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.02.185249v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_158533 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ptag-168::GCaMP6m::rpl-1a
Vector Backbone Description: Backbone Marker:C.I. Bargmann and S. MaCarroll; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574560
Proper citation: RRID:Addgene_158787 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prab-3::nls::GCaMP6m
Vector Backbone Description: Backbone Marker:C.I. Bargmann and S. MaCarroll; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574560
Proper citation: RRID:Addgene_158785 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pntc-1::GCaMP6m::rpl-1a
Vector Backbone Description: Backbone Marker:C.I. Bargmann and S. MaCarroll; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574560
Proper citation: RRID:Addgene_158786 Copy
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