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Species: Homo sapiens
Genetic Insert: WTX
Vector Backbone Description: Backbone Marker:Moon Lab Plasmid available from Addgene (plasmid #15100); Backbone Size:5500; Vector Backbone:pGLUE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17510365
Comments: Learn more about the pGLUE tandem-affinity purification plasmid backbone at http://www.addgene.org/15100/ .
Proper citation: RRID:Addgene_36956 Copy
Species: Homo sapiens
Genetic Insert: EglN2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6886; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36950 Copy
Species: Homo sapiens
Genetic Insert: EglN3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6887; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the lab of a HHMI-investigator
Proper citation: RRID:Addgene_36951 Copy
Vector Backbone Description: Backbone Size:7037; Vector Backbone:pCMMP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18042799
Comments: Sugden lab plasmid p3048
Proper citation: RRID:Addgene_36952 Copy
Species: Homo sapiens
Genetic Insert: WTX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17510365
Proper citation: RRID:Addgene_36958 Copy
Species: Homo sapiens
Genetic Insert: DNMT3B2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15861382
Comments: The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI restriction site (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917.
The full-length cDNA for human DNMT3B2 was cloned into EcoRI and BamHI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen.
Proper citation: RRID:Addgene_36942 Copy
Species: Homo sapiens
Genetic Insert: Thioredoxin 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22430737
Proper citation: RRID:Addgene_36944 Copy
Species: Homo sapiens
Genetic Insert: Thioredoxin-1 C35S mutation
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22430737
Proper citation: RRID:Addgene_36947 Copy
Species: Aequorea victoria
Genetic Insert: Venus (EYFP)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6887; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22343896
Comments: this plasmid was constructed in the laboratory of an HHMI investigator
Proper citation: RRID:Addgene_36948 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: COF1-RFP
Vector Backbone Description: Vector Backbone:pRS416-URA,Amp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20332110
Proper citation: RRID:Addgene_37103 Copy
Species: Homo sapiens
Genetic Insert: CDKN2A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22249253
Proper citation: RRID:Addgene_37104 Copy
Species: Homo sapiens
Genetic Insert: Rb
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7800; Vector Backbone:pQCXIH; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22249253
Comments: Also known as PSM.7LP (Knudsen and Wang 1997 MCB 17:5771).
Proper citation: RRID:Addgene_37106 Copy
Species: Homo sapiens
Genetic Insert: PHLPP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454620
Comments: Please note that the insert in this plasmid was referred to as PHLPP1beta in the original publication (Brognard et al., Mol. Cell 2007) and the plasmid was first described in Warfel et al. 2011.
Proper citation: RRID:Addgene_37100 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: COF1-RFP
Vector Backbone Description: Vector Backbone:pRS413-HIS,Amp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20332110
Proper citation: RRID:Addgene_37101 Copy
Species: Homo sapiens
Genetic Insert: Smad2
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:6900; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20086175
Proper citation: RRID:Addgene_37051 Copy
Species: Homo sapiens
Genetic Insert: HCF-1 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6349; Vector Backbone:pSuper.Retro.Puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371484
Proper citation: RRID:Addgene_36997 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #1764; Backbone Size:4517; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371484
Proper citation: RRID:Addgene_36999 Copy
Species: Homo sapiens
Genetic Insert: THAP11 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6349; Vector Backbone:pSuper.Retro.Puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371484
Proper citation: RRID:Addgene_36993 Copy
Species: Homo sapiens
Genetic Insert: MAML1(1-990)
Vector Backbone Description: Vector Backbone:pHAGE-N-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22547818
Proper citation: RRID:Addgene_37049 Copy
Species: Homo sapiens
Genetic Insert: Smad7
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20086175
Comments: Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro vector.
Proper citation: RRID:Addgene_37044 Copy
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