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Vector Backbone Description: Backbone Marker:Amaxa; Backbone Size:2800; Vector Backbone:pmaxGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:18245377
Proper citation: RRID:Addgene_37631 Copy
Species: Synthetic
Genetic Insert: D4 second generation cameleon (calcium sensor)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16720273
Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and citrine. D4 does not bind wild-type calmodulin. It has a Kd' of 195 microM.
See also Palmer & Tsien (2006) Nature Protocols.
Proper citation: RRID:Addgene_37473 Copy
Species: Drosophila melanogaster
Genetic Insert: Rab4
Vector Backbone Description: Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17409086
Proper citation: RRID:Addgene_37691 Copy
Species: Mus musculus
Genetic Insert: shh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.
Proper citation: RRID:Addgene_37680 Copy
Species: Mus musculus
Genetic Insert: shh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12372301
Comments: The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.
Renilla luciferase was inserted in-frame into the amino-terminal signaling domain located in a peripheral loop within the structure of ShhN.
Proper citation: RRID:Addgene_37677 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19996169
Proper citation: RRID:Addgene_37671 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19996169
Comments: monomeric GFP was constructed by introducing the monomeric mutation, A206K, to EGFP.
Proper citation: RRID:Addgene_37672 Copy
Species: Mus musculus
Genetic Insert: Smo
Vector Backbone Description: Vector Backbone:pEG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10984056
Comments: Addgene NGS results identified a D54N variant compared to the NCBI reference [EDL13766.1].
Proper citation: RRID:Addgene_37674 Copy
Species: Homo sapiens
Genetic Insert: NUMB isoform 2
Vector Backbone Description: Backbone Marker:Beyer et al., J Virol 2002; Backbone Size:7309; Vector Backbone:M107; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21939656
Proper citation: RRID:Addgene_37803 Copy
Species: H. strain TP009
Genetic Insert: ArchT-GFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5088; Vector Backbone:AAV with CaMKII promoter; Vector Types:Mammalian Expression, AAV, Adeno-associated virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21811444
Comments: PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.
Proper citation: RRID:Addgene_37807 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Though there are some discrepancies between the depositor sequence and Addgene's quality control sequence, they are not known to affect plasmid function.
Proper citation: RRID:Addgene_37855 Copy
Species: Simian Virus 40
Genetic Insert: SV40 ST
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37858 Copy
Species: codon-modified firefly (Photinus pyralis)
Genetic Insert: 3 copies of ERRE upstream of luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3-Promoter; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24684682
Proper citation: RRID:Addgene_37851 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:E. coli bacterial strain; Bacterial Resistance:None
Defining Citation: PMID:21110891
Proper citation: RRID:Addgene_37854 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17086198
Proper citation: RRID:Addgene_37843 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BARF1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Though there are some discrepancies between the depositor sequence and Addgene's quality control sequence, they are not known to affect plasmid function.
Proper citation: RRID:Addgene_37922 Copy
Species: HPV
Genetic Insert: HPV 18E6
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37884 Copy
Species: HPV
Genetic Insert: HPV 18 E7
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37886 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #3
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37927 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #1
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37928 Copy
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