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Species: Mus musculus
Genetic Insert: VCP T316V
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12347 Copy
Species: Mus musculus
Genetic Insert: VCP R313A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12346 Copy
Species: Mus musculus
Genetic Insert: VCP H317N
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12349 Copy
Species: Mus musculus
Genetic Insert: VCP H317A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12350 Copy
Species: Mus musculus
Genetic Insert: VCP E319L
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12351 Copy
Species: Mus musculus
Genetic Insert: autocrine motility factor receptor
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123590 Copy
Species: Mus musculus
Genetic Insert: glycogen synthase kinase 3 beta
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123592 Copy
Species: Mus musculus
Genetic Insert: low density lipoprotein receptor-related protein 6
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123595 Copy
Species: Mus musculus
Genetic Insert: catenin (cadherin associated protein), beta 1
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123586 Copy
Species: Mus musculus
Genetic Insert: VCP D598N
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12365 Copy
Species: Mus musculus
Genetic Insert: VCP D592N
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12364 Copy
Species: Mus musculus
Genetic Insert: VCP R599K
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12367 Copy
Species: Mus musculus
Genetic Insert: VCP E578Q
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12361 Copy
Species: Mus musculus
Genetic Insert: VCP R586K
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12363 Copy
Species: Mus musculus
Genetic Insert: VCP R586A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12362 Copy
Species: Mus musculus
Genetic Insert: VCP R362A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12357 Copy
Species: Mus musculus
Genetic Insert: VCP R638A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12368 Copy
Species: Mus musculus
Genetic Insert: VCP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16713576
Proper citation: RRID:Addgene_12373 Copy
Species: Mus musculus
Genetic Insert: mSOD2 shRNA
Vector Backbone Description: Backbone Size:7700; Vector Backbone:UbC-TRE-tTS-IRES-EGFP; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16869515
Comments: See Additional Documents link above for cloning protocol, map, and annotated sequence from the author.
Note that there is a EcoRI and a KpnI site in the polylinker region. You will need to cut the plasmid with BamHI and religate to eliminate these sites before you could EcoRI and KpnI for cloning.
Proper citation: RRID:Addgene_12390 Copy
Species: Mus musculus
Genetic Insert: transferrin receptor
Vector Backbone Description: Backbone Marker:Pharmingen; Backbone Size:9761; Vector Backbone:pAcGP67A; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: The murine TfR sequence bp 367-2292 (of the ORF) was cloned into the pAcGP67A vector. The construct includes a short 3' UTR (past the stop codon at bp 2292). The TfR fragment was PCR amplified to introduce an EcoRI site at the 5' end and an EagI site at the 3' end. This was subsequently cloned into the corresponding EcoRI/EagI sites of pAcGP67A. The murine TfR was fused 3' to a gene segment encoding the hydrophobic leader peptide from the baculovirus protein gp67, a 6X-His tag, and a factor Xa cleavage site.
Proper citation: RRID:Addgene_12392 Copy
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