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Species: Synthetic
Genetic Insert: G(TP)4TG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137101 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: AAC66700.1
Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31772280
Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli. BB0323 is a lipoprotein. BB0323 is not expected to be lipidated in this construct. After TEV cleavage, the target protein has an added A residue at the N-terminus of the mature protein.
Proper citation: RRID:Addgene_137067 Copy
Species: Synthetic
Genetic Insert: GGASPAGG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137102 Copy
Species: Synthetic
Genetic Insert: GGA(EAAAK)2AGG
Vector Backbone Description: Vector Backbone:pL2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137104 Copy
Species: Synthetic
Genetic Insert: FKBP12
Vector Backbone Description: Vector Backbone:pFD; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137108 Copy
Species: Borrelia burgdorferi B31
Genetic Insert: BB0323
Vector Backbone Description: Vector Backbone:pCC1-4k; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31772280
Comments: For use as a PCR template for the expression optimized genes encoding BB0323, P13, DipA, Lmp1. RK2 origin of replication.
Proper citation: RRID:Addgene_137070 Copy
Genetic Insert: mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS-T GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32072132
Comments: If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected].
Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_137075 Copy
Genetic Insert: mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32072132
Comments: If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected].
Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_137074 Copy
Genetic Insert: mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS-T GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32072132
Comments: If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected].
Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_137077 Copy
Genetic Insert: mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pMS-T GeneArt® cloning vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32072132
Comments: If you have any queries or discover any errors, please contact Sherif El-Sharnouby at [email protected] or [email protected].
Please visit https://www.biorxiv.org/content/10.1101/649160v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_137076 Copy
Species: Homo sapiens
Genetic Insert: HPV 16 early region
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pB-actin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2476573
Proper citation: RRID:Addgene_13711 Copy
Species: Synthetic
Genetic Insert: AI(TVMV)
Vector Backbone Description: Vector Backbone:pFD; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137111 Copy
Species: Synthetic
Genetic Insert: mNeongreen-(GGS)2-Cfa(N)
Vector Backbone Description: Vector Backbone:pFLinkC-XE; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31925441
Comments: mNeongreen as negative selection marker
Proper citation: RRID:Addgene_137114 Copy
Species: Synthetic
Genetic Insert: AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV
Vector Backbone Description: Vector Backbone:pFLinkC-XE; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31925441
Proper citation: RRID:Addgene_137117 Copy
Species: Homo sapiens
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:3168; Vector Backbone:pUC19; Vector Types:Bacterial Expression, PCR template for donor DNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31710422
Proper citation: RRID:Addgene_137086 Copy
Species: Homo sapiens
Genetic Insert: tagRFP
Vector Backbone Description: Backbone Size:3168; Vector Backbone:pUC19; Vector Types:Bacterial Expression, PCR template for donor DNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31710422
Proper citation: RRID:Addgene_137088 Copy
Species: Synthetic
Genetic Insert: Con/Foff 2.0-GCaMP6F
Vector Backbone Description: Backbone Size:5300; Vector Backbone:AAV2-EF1a-WPRE; Vector Types:AAV, Cre/Lox, Synthetic Biology, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Comments: Additional sequencing primers: Intron 1F GGGACGACATGACTTAACCAG; Intron 1R CCAGCCCTTCTCATGTTCAG
Proper citation: RRID:Addgene_137123 Copy
Species: Synthetic
Genetic Insert: Con/Fon-GCaMP6F
Vector Backbone Description: Backbone Size:5300; Vector Backbone:AAV2-EF1a-WPRE; Vector Types:AAV, Cre/Lox, Synthetic Biology, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Comments: Additional sequencing primers: Intron 1F GGGACGACATGACTTAACCAG; Intron 1R CCAGCCCTTCTCATGTTCAG
Proper citation: RRID:Addgene_137122 Copy
Species: Synthetic
Genetic Insert: sRGECO
Vector Backbone Description: Backbone Size:5300; Vector Backbone:AAV2-EF1a-WPRE; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Proper citation: RRID:Addgene_137125 Copy
Species: Synthetic
Genetic Insert: Coff/Fon-GCaMP6F
Vector Backbone Description: Backbone Size:5300; Vector Backbone:AAV2-EF1a-WPRE; Vector Types:AAV, Cre/Lox, Synthetic Biology, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574559
Comments: Additional sequencing primers: Intron 1F GGGACGACATGACTTAACCAG; Intron 1R CCAGCCCTTCTCATGTTCAG
Proper citation: RRID:Addgene_137124 Copy
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