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Species: Mus musculus
Genetic Insert: mmu-mir-138-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26328 Copy
Species: Mus musculus
Genetic Insert: mmu-mir-137
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26327 Copy
Species: Homo sapiens
Genetic Insert: cand181
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26323 Copy
Species: Homo sapiens
Genetic Insert: cand142
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26322 Copy
Species: Mus musculus
Genetic Insert: mmu-mir-1197
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26339 Copy
Species: Mus musculus
Genetic Insert: mmu-mir-224
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26335 Copy
Species: Mus musculus
Genetic Insert: mmu-mir-375
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26336 Copy
Species: Mus musculus
Genetic Insert: mmu-mir-153
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20413612
Comments: pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
Proper citation: RRID:Addgene_26330 Copy
Species: Homo sapiens
Genetic Insert: Mediator of DNA damage checkpoint 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2477; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_26427 Copy
Genetic Insert: Tetracycline repressor A3 mutant
Vector Backbone Description: Backbone Size:7681; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Use to make them Tet-On Advanced.
Proper citation: RRID:Addgene_26429 Copy
Species: Mus musculus
Genetic Insert: partial sequence of mouse Titin
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pRRL.SIN.cPPT.PGK.GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_26428 Copy
Species: E.coli, HSV
Genetic Insert: HSV-TK, mCMV-b-gal
Vector Backbone Description: Backbone Size:3360; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Comments: There are some discrepancies between Addgene's quality control sequence and the author's sequence, but the Mitani lab has confirmed that this plasmid is correct. The depositor has also confirmed that there is an extra SacI site present in the plasmid is not apparent in the theoretical sequence on which the map below is based. Addgene does not recommend using SacI for a diagnostic digest.
Proper citation: RRID:Addgene_26421 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26387 Copy
Species: Homo sapiens
Genetic Insert: FUS
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5299; Vector Backbone:pFRT_DESTFLAGHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081015
Proper citation: RRID:Addgene_26381 Copy
Species: unknown
Genetic Insert: bActin promoter
Vector Backbone Description: Backbone Size:4726; Vector Backbone:unknown; Vector Types:4726; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_26437 Copy
Genetic Insert: no insert
Vector Backbone Description: Backbone Size:9116; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced into a Tet-On Advanced cell line for expression of the GOI.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.
Proper citation: RRID:Addgene_26434 Copy
Species: Danio rerio
Genetic Insert: flk1 promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Topo cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16251212
Comments: From the paper:
Approximately 6.5 kb of upstream sequence of the zebrafish flk1 gene (Liao et al., 1997; Thompson et al., 1998) was amplified from wild-type genomic DNA based on the sequence information previously deposited (from –6410 to –3 of the transcriptional start site, GenBank Accession Number AY045466) (Chan et al., 2002), and subcloned into the pCRII TOPO vector (Invitrogen). This fragment has been shown to drive endothelial-specific expression of the green-red coral fluorescent protein (G-RCFP) (Cross et al., 2003). The EcoRI cleaved fragment from this construct was placed 5′ upstream of the enhanced green fluorescent protein (EGFP) vector (Clontech) to drive endothelial specific expression of EGFP
Proper citation: RRID:Addgene_26436 Copy
Species: Mus musculus
Genetic Insert: cytoplasmic dynein 1 intermediate chain 1 isoform B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:subcloning and sequencing; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20657784
Proper citation: RRID:Addgene_26435 Copy
Genetic Insert: no insert
Vector Backbone Description: Backbone Size:9402; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced in a Tet-On advanced cell line to obtain expression of the GOI.
Proper citation: RRID:Addgene_26430 Copy
Genetic Insert: Cerulean-32-Venus
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:3937; Vector Backbone:EGFP N1; Vector Types:Mammalian Expression, FRET standard; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17040988
Comments: This construct contains Cerulean attached via a 32 amino acid linker to Venus. See supplemental methods section in associated paper for further details of plasmid construction.
Proper citation: RRID:Addgene_26396 Copy
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