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Species: Homo sapiens
Genetic Insert: CIRTS-FTO-Calm3-U6-Malat1 gRNA
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:9801; Vector Backbone:pFsy(1.1)GW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37669863
Proper citation: RRID:Addgene_216860 Copy
Species: Homo sapiens
Genetic Insert: hM3D(Gq)-mCherry
Vector Backbone Description: Backbone Size:4916; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Created by inserting hM3D(Gq)-mCherry in the pAAV-hSyn-frt-DIO-hM4D(Gi)-mCherry plasmid from this paper (Addgene Plasmid #XXXXX)
Proper citation: RRID:Addgene_217397 Copy
Species: Homo sapiens
Genetic Insert: CRYGA
Vector Backbone Description: Backbone Marker:LifeSensors; Backbone Size:5761; Vector Backbone:PE-SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39368693
Comments: Expresses well in a soluble state. However, the SUMO tag is not removed by ULP-1 cleavage. The N-terminal methionine has been removed to mirror the form of the protein in lens that does not contain its methionine.
Proper citation: RRID:Addgene_217672 Copy
Species: Homo sapiens
Genetic Insert: PARP1 catalytic domain (no Regulatory Domain)
Vector Backbone Description: Vector Backbone:pNIC-CFP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38365924
Comments: CFP-TEV site-PARP1 cat (no RD), PARP inhibitor 3-Amino Benzamide (2 mM) is required during growth.
Plasmid mutates easily and depositor recommends routine sequencing of at least the catalytic domain for each miniprep that you prepare.
Proper citation: RRID:Addgene_216872 Copy
Species: Homo sapiens
Genetic Insert: Full length HPF1
Vector Backbone Description: Vector Backbone:pNIC-YFP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38365924
Comments: YFP-TEV site-HPF1 (full length)
Proper citation: RRID:Addgene_216874 Copy
Species: Homo sapiens
Genetic Insert: Frb-NLS-mCherry
Vector Backbone Description: Vector Backbone:pHR-ssFv_lenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38315854
Proper citation: RRID:Addgene_217613 Copy
Species: Homo sapiens
Genetic Insert: melanoma differentiation-associated protein 5 delta 2 CARD domains
Vector Backbone Description: Vector Backbone:pFLAG-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_216801 Copy
Species: Homo sapiens
Genetic Insert: ZIF-NLS-EGFP*-HOTag6-T2A-CEL-EGFP-YAP
Vector Backbone Description: Vector Backbone:pHR-ssFv_lenti; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37521779
Proper citation: RRID:Addgene_217611 Copy
Species: Homo sapiens
Genetic Insert: shWT1_B
Vector Backbone Description: Backbone Marker:Dharmacon # RHS4744; Vector Backbone:pTRIPZ; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Bleocin (Zeocin)
Defining Citation: PMID:33882316
Proper citation: RRID:Addgene_217637 Copy
Species: Homo sapiens
Genetic Insert: Kinase suppressor of ras 1 CA3 domain
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4600; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Sleeping Beauty Transposase-compatible genome insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38474242
Proper citation: RRID:Addgene_217769 Copy
Species: Homo sapiens
Genetic Insert: CIP2A
Vector Backbone Description: Backbone Size:4966; Vector Backbone:pGEX-4T-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_217924 Copy
Species: Homo sapiens
Genetic Insert: Kinase suppressor of ras 1 CA3 domain
Vector Backbone Description: Backbone Marker:EMBL; Backbone Size:6290; Vector Backbone:pETM11-SUMO3; Vector Types:Bacterial Expression, Protein purification (6xHis tag, TEV site, SUMO3 tag); Bacterial Resistance:Kanamycin
Defining Citation: PMID:38474242
Comments: https://www.embl.org/groups/protein-expression-purification/services/strategy-and-construct-design/e-coli-expression-vectors/cloning-in-the-petm11-sumo3-egfp-vector/
Proper citation: RRID:Addgene_217767 Copy
Species: Homo sapiens
Genetic Insert: Kinase suppressor of ras 1 CA3 domain
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4600; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Sleeping Beauty Transposase-compatible genome insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38474242
Proper citation: RRID:Addgene_217768 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase C zeta
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4600; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38474242
Proper citation: RRID:Addgene_217761 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase C zeta
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4600; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38474242
Proper citation: RRID:Addgene_217762 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase C zeta
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4600; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38474242
Proper citation: RRID:Addgene_217760 Copy
Species: Homo sapiens
Genetic Insert: SET
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4600; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38474242
Proper citation: RRID:Addgene_217765 Copy
Species: Homo sapiens
Genetic Insert: Kinase suppressor of ras 1 CA3 domain
Vector Backbone Description: Backbone Marker:EMBL; Backbone Size:6290; Vector Backbone:pETM11-SUMO3; Vector Types:Bacterial Expression, Protein purification (6xHis tag, TEV site, SUMO3 tag); Bacterial Resistance:Kanamycin
Defining Citation: PMID:38474242
Comments: https://www.embl.org/groups/protein-expression-purification/services/strategy-and-construct-design/e-coli-expression-vectors/cloning-in-the-petm11-sumo3-egfp-vector/
Proper citation: RRID:Addgene_217766 Copy
Species: Homo sapiens
Genetic Insert: Protein kinase C zeta
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4600; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38474242
Proper citation: RRID:Addgene_217763 Copy
Species: Homo sapiens
Genetic Insert: SET
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4600; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38474242
Proper citation: RRID:Addgene_217764 Copy
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