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Species: Schizosaccharomyces pombe
Genetic Insert: cdc8
Vector Backbone Description: Vector Backbone:pJC20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29080743
Proper citation: RRID:Addgene_99361 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: cdc8
Vector Backbone Description: Vector Backbone:pJC20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29080743
Proper citation: RRID:Addgene_99363 Copy
Species: phage lambda
Genetic Insert: phage lambda gene R
Vector Backbone Description: Backbone Marker:Jay Keasling lab; Vector Backbone:pPro24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28795570
Comments: pAD-LyseR plasmid in BL21-Gold (DE3) strain is used for producing autolysate for in vitro transcription-translation reactions.
Proper citation: RRID:Addgene_99244 Copy
Vector Backbone Description: Vector Backbone:no vector; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28795570
Comments: The strain is used for producing bacterial lysates with no beta-galactosidase activity.
Proper citation: RRID:Addgene_99247 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2851; Vector Backbone:hSTARR-seq_SCP1 vector; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech) . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99292 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2537; Vector Backbone:hSTARR-seq_ORI vector; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech). In this vector the origin of replication (ORI) substitutes a core promoter . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99296 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3983; Vector Backbone:STARR-seq luciferase validation vector_ORI_empty; Vector Types:Mammalian Expression, Luciferase, STARR-seq Luciferase validation vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29256496
Comments: This vector serves as a control for Luciferase assays. The origin of replication substitutes the core promoter; there is no enhancer cloned into this construct . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99297 Copy
Species: Mus musculus
Genetic Insert: CBP KIX (586-672)
Vector Backbone Description: Backbone Marker:Novaen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10222196
Proper citation: RRID:Addgene_99336 Copy
Species: Mus musculus
Genetic Insert: mouse CBP HAT domain (1324-1700)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5493; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28630323
Proper citation: RRID:Addgene_99340 Copy
Species: Homo sapiens
Genetic Insert: USP7
Vector Backbone Description: Vector Backbone:pMH-SFB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28325877
Proper citation: RRID:Addgene_99393 Copy
Species: Homo sapiens
Genetic Insert: BRCA2
Vector Backbone Description: Vector Backbone:pMH-SFB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19369211
Comments: The BRCA2 gene in this plasmid contains D2005G, E2175G, and I3286V mutations. The depositor confirms that these have no affect on plasmid function.
Proper citation: RRID:Addgene_99395 Copy
Species: synthetic; S. pyogenes
Genetic Insert: dCas9-KRAB-T2A-Puro
Vector Backbone Description: Backbone Size:9192; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757163
Comments: dCas9 sequence is from Addgene plasmid #61425 (generously shared by Feng Zhang lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014
KRAB sequence is from Addgene plasmid #50919 (generously shared by Rene Maehr, Scot Wolfe lab). "Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.", Kearns et al. 2014
Proper citation: RRID:Addgene_99372 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Backbone Size:9465; Vector Backbone:lentiGuide ( plasmid #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757163
Comments: Hygro sequence is from Addgene plasmid #61426 (generously shared by Feng Zhang Lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014
Proper citation: RRID:Addgene_99375 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Backbone Size:9465; Vector Backbone:lentiGuide ( plasmid #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757163
Comments: Hygro sequence is from Addgene plasmid #61426 (generously shared by Feng Zhang Lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014
mTagBFP2 sequence is from Addgene plasmid #34632 (generously shared by Vladislav Verkhusha Lab). "An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. ", Subach et al.. 2014
Proper citation: RRID:Addgene_99374 Copy
Species: Synthetic
Genetic Insert: S. pyogenes sgRNA cassette
Vector Backbone Description: Backbone Size:9465; Vector Backbone:lentiGuide ( plasmid #52963); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757163
Comments: Hygro sequence is from Addgene plasmid #61426 (generously shared by Feng Zhang Lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014
iRFP670 sequence is codon-optimized for human (its original sequence is from Addgene plasmid #45453; generously shared by Vladislav Verkhusha Lab). "Near-infrared fluorescent proteins for multicolor in vivo imaging.", Shcherbakova et al.. 2013
Proper citation: RRID:Addgene_99377 Copy
Species: Arabidopsis thaliana
Genetic Insert: TIR1
Vector Backbone Description: Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Digest this vector with EcoRV for integration into TRP1.
Addgene NGS results found a single nucleotide change compared to NM_116163.4 that results in T591I within the TIR1 translation. The protein is noted by the depositing laboratory to be functional in vivo
Proper citation: RRID:Addgene_99532 Copy
Species: Homo sapiens
Genetic Insert: DLC1-isoform 4
Vector Backbone Description: Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21217778
Proper citation: RRID:Addgene_99534 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19107412
Comments: The ORF is followed by the ADH1 transcription terminator. Please see secondary publication PMID: 22941627 for more information about this plasmid.
Proper citation: RRID:Addgene_99536 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:5241; Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19107412
Comments: The ORF is followed by the ADH1 transcription terminator. Please see secondary publication PMID: 22941627 for more information about this plasmid.
Proper citation: RRID:Addgene_99538 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by 1129 bp of the POL30 3' region; digest with StuI for integration into URA3 or with PmlI for integration upstream of endogenous POL30 (for exchange of alleles by 5FOA selection.) Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99546 Copy
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