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Species: Synthetic
Genetic Insert: PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
Vector Backbone Description: Vector Backbone:pCMV-T7-SpCas9-P2A-EGFP (RTW3027); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39039307
Comments: Please visit https://doi.org/10.1101/2023.09.12.557440 for bioRxiv preprint.
Proper citation: RRID:Addgene_208962 Copy
Species: Synthetic
Genetic Insert: B103-BPNLS-CC(N5)
Vector Backbone Description: Vector Backbone:pCMV-T7-SpCas9-P2A-EGFP (RTW3027); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39039307
Comments: Please visit https://doi.org/10.1101/2023.09.12.557440 for bioRxiv preprint.
Proper citation: RRID:Addgene_208974 Copy
Species: Synthetic
Genetic Insert: ePhi29(+exo)-BPNLS-CC(N5)
Vector Backbone Description: Vector Backbone:pCMV-T7-SpCas9-P2A-EGFP (RTW3027); Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39039307
Comments: Please visit https://doi.org/10.1101/2023.09.12.557440 for bioRxiv preprint.
Proper citation: RRID:Addgene_208972 Copy
Species: Synthetic
Genetic Insert: Bxb1
Vector Backbone Description: Backbone Size:2093; Vector Backbone:pZa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35200383
Proper citation: RRID:Addgene_204686 Copy
Species: Synthetic
Genetic Insert: Myc-tag
Vector Backbone Description: Backbone Marker:Addgene ID: 206144; Backbone Size:8264; Vector Backbone:mTB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37679620
Proper citation: RRID:Addgene_210205 Copy
Species: Synthetic
Genetic Insert: aδNb072
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5645; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37987253
Proper citation: RRID:Addgene_204627 Copy
Species: Synthetic
Genetic Insert: SUMO-A'-l2-A'-IRES-EmGFP
Vector Backbone Description: Vector Backbone:pHR-PCMV-TetO2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37919273
Comments: CC: coiled-coil; GEMS: Generalized Extracellular Molecule Sensor. Please visit https://doi.org/10.1101/2023.01.18.524631 for bioRxiv preprint.
Proper citation: RRID:Addgene_209125 Copy
Species: Synthetic
Genetic Insert: SUMO-A'-l4-G'-IRES-EmGFP
Vector Backbone Description: Vector Backbone:pHR-PCMV-TetO2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37919273
Comments: CC: coiled-coil; GEMS: Generalized Extracellular Molecule Sensor. Please visit https://doi.org/10.1101/2023.01.18.524631 for bioRxiv preprint.
Proper citation: RRID:Addgene_209127 Copy
Species: Synthetic
Genetic Insert: BirAopt
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: Expresses optimized BirA only (with short C-terminal fusion derived from GSQ linker, RBXN polylinker, and P2A remnant). For performing BioE3 experiments; can be used for transient expression or packaged into lentiviral particles for stable cell line selection.
Proper citation: RRID:Addgene_208048 Copy
Species: Synthetic
Genetic Insert: pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
Vector Backbone Description: Vector Backbone:pegRNA entry vector (MNW320); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39039307
Comments: Please visit https://doi.org/10.1101/2023.09.12.557440 for bioRxiv preprint.
Proper citation: RRID:Addgene_208977 Copy
Species: Synthetic
Genetic Insert: T7RNAP Circuit
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35366417
Proper citation: RRID:Addgene_199992 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52961); Backbone Size:14873; Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37683180
Proper citation: RRID:Addgene_209750 Copy
Species: Synthetic
Genetic Insert: EGFP/BC12
Vector Backbone Description: Vector Backbone:pCER206; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37321219
Comments: Please note: Plasmid contains a M234K mutation in EGFP. This mutation is not expected to affect plasmid function.
Proper citation: RRID:Addgene_202853 Copy
Species: Synthetic
Genetic Insert: NIP45 wt siRES
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO/EGFP-N; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37474739
Proper citation: RRID:Addgene_210262 Copy
Species: Synthetic
Genetic Insert: NIP45 D394R siRES
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO/mCherry-N; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37474739
Proper citation: RRID:Addgene_210267 Copy
Species: Synthetic
Genetic Insert: NIP45 D394R siRES
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO/EGFP-N; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37474739
Proper citation: RRID:Addgene_210265 Copy
Species: Synthetic
Genetic Insert: NIP45 wt siRES
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO/mCherry-N; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37474739
Proper citation: RRID:Addgene_210266 Copy
Species: Synthetic
Genetic Insert: RFP and four sgRNA GBX2
Vector Backbone Description: Vector Backbone:pLV-GG-hUbC-dsRED; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38052784
Proper citation: RRID:Addgene_192288 Copy
Species: Synthetic
Genetic Insert: pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
Vector Backbone Description: Vector Backbone:HEpl016; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37231266
Proper citation: RRID:Addgene_205412 Copy
Species: Synthetic
Genetic Insert: EGFP/BC17
Vector Backbone Description: Vector Backbone:pCER206; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37321219
Comments: A frameshift mutation exists in eGFP and may affect expression of the eGFP, but it does not affect the 10nt barcode's expression as determined by sequencing-based methods
Proper citation: RRID:Addgene_202858 Copy
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