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Genetic Insert: FRB-Draper INT domain mCherry
Vector Backbone Description: Vector Backbone:pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30139739
Proper citation: RRID:Addgene_126666 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Derived in Kotton lab; Backbone Size:6977; Vector Backbone:pHAGE2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28366587
Proper citation: RRID:Addgene_126703 Copy
Genetic Insert: Draper
Vector Backbone Description: Vector Backbone:pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30139739
Proper citation: RRID:Addgene_126653 Copy
Species: Salmonella typhimurium ATCC14028 Nal resistant
Genetic Insert: adrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
Vector Backbone Description: Backbone Marker:Guzman et al, J Bacteriol, 1995, 177(14):4121-30; Backbone Size:4919; Vector Backbone:pBAD30; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:15306016
Proper citation: RRID:Addgene_126650 Copy
Genetic Insert: Draper with Cytoplasmic ITAM residues Tyr all others mutated to Phe
Vector Backbone Description: Vector Backbone:pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30139739
Proper citation: RRID:Addgene_126656 Copy
Genetic Insert: Shark
Vector Backbone Description: Vector Backbone:pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30139739
Proper citation: RRID:Addgene_126658 Copy
Genetic Insert: Crk
Vector Backbone Description: Vector Backbone:pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30139739
Proper citation: RRID:Addgene_126659 Copy
Species: Synthetic
Genetic Insert: LbCas12a Full Length Direct Repeat crRNA
Vector Backbone Description: Backbone Size:3659; Vector Backbone:pBCSK-; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30892626
Comments: For cloning in crRNAs: 5p oligo needs AGAT on 5' end. 3p oligo needs AAAA on 5' end. Matches BbsI overhangs
Proper citation: RRID:Addgene_126641 Copy
Species: Synthetic
Genetic Insert: FnoCas12a Full Length Direct Repeat crRNA
Vector Backbone Description: Backbone Size:3659; Vector Backbone:pBCSK-; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30892626
Comments: For cloning in crRNAs: 5p oligo needs AGAT on 5' end. 3p oligo needs AAAA on 5' end. Matches BbsI overhangs
Proper citation: RRID:Addgene_126642 Copy
Species: Synthetic
Genetic Insert: AsCas12a Full Length Direct Repeat crRNA
Vector Backbone Description: Backbone Size:3659; Vector Backbone:pBCSK-; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30892626
Comments: For cloning in crRNAs: 5p oligo needs AGAT on 5' end. 3p oligo needs AAAA on 5' end. Matches BbsI overhangs
Proper citation: RRID:Addgene_126640 Copy
Species: Synthetic
Genetic Insert: LbCas12a Full Length Direct Repeat GC Swap crRNA
Vector Backbone Description: Backbone Size:3232; Vector Backbone:pBCSK+; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30892626
Comments: For cloning crRNA guide sequences into the GC swap frameworks: The top oligo should contain a GTGG overhang on its 5' end and the bottom oligo should contain AAAA overhang on its 5' end to match the overhangs that are produced by BbsI. In addition, for these constructs the guide oligos should encode the final two bases [GT] of the constant region of the crRNA prior to the spacer [targeting] sequence and then two additional Ts following the spacer sequence to ensure robust RNA Polymerase III termination.
Proper citation: RRID:Addgene_126643 Copy
Genetic Insert: SurE
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET-21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524683
Proper citation: RRID:Addgene_12685 Copy
Species: Helicobacter pylori
Genetic Insert: UDP-N-acetylglucosamine acyltransferase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET-21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14579368
Proper citation: RRID:Addgene_12684 Copy
Species: Helicobacter pylori
Genetic Insert: type II dehydroquinase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET-21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12784220
Proper citation: RRID:Addgene_12682 Copy
Species: Homo sapiens
Genetic Insert: RAB11A (Homo sapiens)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12070301
Proper citation: RRID:Addgene_12680 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ALD6
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27419466
Proper citation: RRID:Addgene_126751 Copy
Genetic Insert: yEGFP
Vector Backbone Description: Vector Backbone:pRS413; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27419466
Proper citation: RRID:Addgene_126750 Copy
Species: S. pyogenes
Genetic Insert: WT SpCas9
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32144253
Comments: pCbh-3xFLAG-NLS-WT SpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations).
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126753 Copy
Species: S. pyogenes
Genetic Insert: eSpCas9
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32144253
Comments: pCbh-3xFLAG-NLS-eSpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations).
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126754 Copy
Species: S. pyogenes
Genetic Insert: evoSpCas9
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32144253
Comments: pCbh-3xFLAG-NLS-evoSpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations).
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126758 Copy
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