Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pDECKO_mCherry_LINC02432 Resource Report Resource Website |
RRID:Addgene_140107 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-29 12:56:36 | 0 | ||
|
mVenus-hCOL1A1 Resource Report Resource Website |
RRID:Addgene_140111 | Type I procollagen α1 | Homo sapiens | Ampicillin | These human type I procollagen plasmids were designed and validated for human osteoblasts and fibroblasts similar to their previously published/shared murine counterparts e.g. eGFP-Proα1 (Plasmid #119843) https://www.addgene.org/Sergey_Leikin/. Their expression in other cells might disrupt assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in human osteoblasts and fibroblasts, overexpression of exogenous proα2 might cause intracellular trafficking and degradation of some transfected chains as monomers without integration into normal heterotrimeric (proα1)2proα2 procollagen molecules,. Overexpression of exogenous proα1 might result in excessive formation of homotrimeric (proα1)3 molecules, some of which might contain fluorescent tags on two or even all three proα1 chains, potentially causing severe disruptions in cellular function. In our experience with the murine plasmids expressed in primary osteoblasts or the MC3T3 osteoblastic cell line, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12-24 h after transfection. These were harder to observe in human Saos2 osteoblast cell line transfected with the human type I procollagen plasmids (likely due to slower fibrillogenesis and more complete cleavage of the fluorescently-tagged N-propeptide prior to collagen fibril formation). We strongly recommend following these guidelines when using the plasmids 1) Only human cells that have high expression of endogenous type I procollagen and that express fewer transfected than endogenous chains are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Stable transfection causes excessive accumulation of tagged chains in the ER over time, disruption of ER homeostasis, and disruptions and changes in cellular function. Therefore, low to moderate transient transfection is recommended for studies of physiologically relevant processes. Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid the excessive accumulation of tagged chains in the ER and cell malfunction caused by increased procollagen synthesis. 5) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | COL1A1 exons 2-3 replaced by fluorescent tag | 2026-08-29 12:56:32 | 0 | |
|
mCerulean-hCOL1A1 Resource Report Resource Website |
RRID:Addgene_140112 | Type I procollagen α1 | Homo sapiens | Ampicillin | These human type I procollagen plasmids were designed and validated for human osteoblasts and fibroblasts similar to their previously published/shared murine counterparts e.g. eGFP-Proα1 (Plasmid #119843) https://www.addgene.org/Sergey_Leikin/. Their expression in other cells might disrupt assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in human osteoblasts and fibroblasts, overexpression of exogenous proα2 might cause intracellular trafficking and degradation of some transfected chains as monomers without integration into normal heterotrimeric (proα1)2proα2 procollagen molecules,. Overexpression of exogenous proα1 might result in excessive formation of homotrimeric (proα1)3 molecules, some of which might contain fluorescent tags on two or even all three proα1 chains, potentially causing severe disruptions in cellular function. In our experience with the murine plasmids expressed in primary osteoblasts or the MC3T3 osteoblastic cell line, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12-24 h after transfection. These were harder to observe in human Saos2 osteoblast cell line transfected with the human type I procollagen plasmids (likely due to slower fibrillogenesis and more complete cleavage of the fluorescently-tagged N-propeptide prior to collagen fibril formation). We strongly recommend following these guidelines when using the plasmids 1) Only human cells that have high expression of endogenous type I procollagen and that express fewer transfected than endogenous chains are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Stable transfection causes excessive accumulation of tagged chains in the ER over time, disruption of ER homeostasis, and disruptions and changes in cellular function. Therefore, low to moderate transient transfection is recommended for studies of physiologically relevant processes. Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid the excessive accumulation of tagged chains in the ER and cell malfunction caused by increased procollagen synthesis. 5) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | COL1A1 exons 2-3 replaced by fluorescent tag | 2026-08-29 12:56:36 | 0 | |
|
pDECKO_mCherry_SPI1 Resource Report Resource Website |
RRID:Addgene_140099 | gRNA1+scaffold+H1promoter+gRNA2 | Homo sapiens | Ampicillin | PMID:35619054 | Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-29 12:56:36 | 0 | ||
|
Wnt10B Non-STOP Resource Report Resource Website |
RRID:Addgene_34748 | Wnt10B | Homo sapiens | Kanamycin | PMID:22784633 | Please note that the ORFs in this collection were cloned open-ended (without a stop codon). | Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | None | 2026-08-29 01:04:15 | 0 |
|
GST-PRMT7-ΔC Resource Report Resource Website |
RRID:Addgene_34695 | PRMT7 | Homo sapiens | Ampicillin | PMID:15044439 | Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Contains aa 1-378; and N375K was introduced with the NotI site | 2026-08-29 01:04:14 | 0 | |
|
GST-PRMT7-ΔN Resource Report Resource Website |
RRID:Addgene_34694 | PRMT7 | Homo sapiens | Ampicillin | PMID:15044439 | Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Contains aa 379–692 | 2026-08-29 01:04:15 | 0 | |
|
GST-PRMT7 Resource Report Resource Website 1+ mentions |
RRID:Addgene_34693 | PRMT7 | Homo sapiens | Ampicillin | PMID:15044439 | Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX(SN); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:14 | 3 | ||
|
pFLAG-TNKS-2-M1054V Resource Report Resource Website |
RRID:Addgene_34692 | TNKS2-M1054V | Homo sapiens | Ampicillin | PMID:11802774 | Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | M1054V | 2026-08-29 01:04:14 | 0 | |
|
wt 6XHis-SNX9 pET15b Resource Report Resource Website 1+ mentions |
RRID:Addgene_34690 | Sorting nexin 9 | Homo sapiens | Ampicillin | PMID:17988218 | Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:14 | 2 | ||
|
K44A HA-dynamin 1 pcDNA3.1 Resource Report Resource Website 10+ mentions |
RRID:Addgene_34683 | dynamin 1ba | Homo sapiens | Ampicillin | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K44A | 2026-08-29 01:04:14 | 10 | ||
|
wt HA-dynamin 1 pUHD10-3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_34688 | dynamin 1 | Homo sapiens | Ampicillin | PMID:7559787 | Vector Backbone:pUHD10-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:15 | 2 | ||
|
wt HA-dynamin 1 pcDNA3.1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_34682 | dynamin 1ba | Homo sapiens | Ampicillin | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:15 | 7 | |||
|
K44A dynamin 1 pEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_34681 | dynamin 1ba | Homo sapiens | Kanamycin | PMID:15004222 | Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | K44A | 2026-08-29 01:04:14 | 8 | |
|
pQTEV-PTD012 Resource Report Resource Website |
RRID:Addgene_34797 | PTD012 | Homo sapiens | Ampicillin | PMID:15998469 | The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence. | Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:15 | 0 | |
|
pcDNA3-CD68 promoter/enhancer Resource Report Resource Website 1+ mentions |
RRID:Addgene_34837 | CD68 promoter/enhancer -680/+140 | Homo sapiens | Ampicillin | PMID:11907098 | The CD68 promoter/enhancer in this construct consists of ~680bp 5' of the ATG and the 83bp first intron of human CD68. Please note that Addgene's sequencing results found a single nucleotide deletion at bp# 440 when compared to the full plasmid sequence provided by the depositor. The depositing scientist states that it is probably a polymorphism in the promoter and is not a concern for the function of the plasmid. | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:16 | 8 | |
|
pQTEV-NOHMA Resource Report Resource Website |
RRID:Addgene_34821 | HORMA domain containing protein | Homo sapiens | Ampicillin | PMID:15998469 | The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence. | Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:15 | 0 | |
|
Human_TFAM_NoMTS_pET28 Resource Report Resource Website 1+ mentions |
RRID:Addgene_34705 | TFAM | Homo sapiens | Kanamycin | PMID:22037171 | Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Encodes residues 43–246, corresponding to full-length TFAM after cleavage of the N-terminal mitochondrial leader sequence (residues 1–42) | 2026-08-29 01:04:15 | 3 | |
|
pQTEV-EPSTI1 Resource Report Resource Website |
RRID:Addgene_34826 | epithelial stromal interaction 1 | Homo sapiens | Ampicillin | PMID:15998469 | The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence. | Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:15 | 0 | |
|
pQTEV-PHYHIPL Resource Report Resource Website |
RRID:Addgene_34823 | phytanoyl-CoA hydroxylase interacting protein-like | Homo sapiens | Ampicillin | PMID:15998469 | The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence. | Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:15 | 0 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
