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Species: Other
Genetic Insert: pHRed
Vector Backbone Description: Backbone Marker:Grefen et al. The Plant Journal (2010) 64, 355–365; Backbone Size:9210; Vector Backbone:pUB-DEST; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28663550
Proper citation: RRID:Addgene_84734 Copy
Species: Synthetic
Genetic Insert: STTM-miR408
Vector Backbone Description: Backbone Marker:Dr. Kang Wang at Iowa State University; Vector Backbone:TF101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23098881
Proper citation: RRID:Addgene_84814 Copy
Species: Synthetic
Genetic Insert: STTM-miR394
Vector Backbone Description: Backbone Marker:Dr. Kang Wang at Iowa State University; Vector Backbone:TF101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23098881
Comments: Please note that bp8 of the provided STTM-miR394 sequence is missing. The depositing lab noted that this missing bp should not affect plasmid function.
Proper citation: RRID:Addgene_84813 Copy
Species: Synthetic
Genetic Insert: STTM-miR165/166
Vector Backbone Description: Backbone Marker:Dr. Kang Wang at Iowa State University; Vector Backbone:TF101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23098881
Proper citation: RRID:Addgene_84816 Copy
Species: Synthetic
Genetic Insert: STTM-miR172
Vector Backbone Description: Backbone Marker:Dr. Kang Wang at Iowa State University; Vector Backbone:TF101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23098881
Proper citation: RRID:Addgene_84815 Copy
Genetic Insert: RpaR
Vector Backbone Description: Vector Backbone:pBjk2807; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27172092
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_85148 Copy
Genetic Insert: LasR
Vector Backbone Description: Vector Backbone:pBjk2807; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27172092
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_85147 Copy
Genetic Insert: TraR
Vector Backbone Description: Vector Backbone:pBjk2807; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27172092
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_85150 Copy
Species: Synechoccus elongatus PCC 7942
Genetic Insert: PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
Vector Backbone Description: Vector Backbone:PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25127213
Proper citation: RRID:Addgene_85122 Copy
Species: Synthetic
Genetic Insert: crRNA of crtYf
Vector Backbone Description: Vector Backbone:pXMJ19 and pTRCmob; Vector Types:Bacterial Expression, CRISPR, Shuttle vector Corynebacterium glutamicum / E. coli; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28469274
Proper citation: RRID:Addgene_85544 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: para-cyanophenylalanine Mj synthetase
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:19492814
Proper citation: RRID:Addgene_85495 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26237426
Proper citation: RRID:Addgene_85497 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:12537491
Proper citation: RRID:Addgene_85503 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:20839808
Comments: This synthetase is permissive and can incorporate a variety of amino acids structurally analogous to IBBN. For a more complete functional and structural analysis of this synthetase, also see Cooley et al. Chembiochem. 2014, 15(12): 1810–1819.
Proper citation: RRID:Addgene_85501 Copy
Species: Synthetic
Genetic Insert: sgRNA targeting firefly luciferase encoding gene
Vector Backbone Description: Backbone Size:3245; Vector Backbone:pPEP1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28490437
Comments: For information of vector pPEP1 and promoter of P3, please refer to
"Sorg RA, Kuipers OP, Veening JW (2014) Gene Expression Platform for Synthetic Biology in the Human Pathogen Streptococcus pneumoniae. ACS synthetic biology"
Proper citation: RRID:Addgene_85590 Copy
Species: Streptococcus pyogenes
Genetic Insert: human-codon-optimized SpCas9
Vector Backbone Description: Backbone Marker:Prof. Ikuko Hara-Nishimura; Vector Backbone:pFAST-R01; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27856772
Comments: Although this vector does not has a problem of sgRNA truncation like pKIR1.1, cloning efficiency in vector construction is low. E. coli with high competency is required.
Proper citation: RRID:Addgene_85808 Copy
Species: Streptococcus pyogenes
Genetic Insert: human-codon-optimized SpCas9
Vector Backbone Description: Backbone Marker:Prof. Ikuko Hara-Nishimura; Vector Backbone:pFAST-R01; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27856772
Comments: Right border (RB) may be truncated in the T-DNA integration. This may result in sgRNA scaffold truncation because the RB is close to 3' end of sgRNA. However, since we efficiently obtained knockout mutants by this vector, the truncation is not serious problem.
To overcome it, we also deposited pKI1.1R. In pKI1.1R, sgRNA is not close to RB.
Proper citation: RRID:Addgene_85758 Copy
Species: Homo sapiens
Genetic Insert: ZBTB24
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27478040
Comments: CLOSED (stop codon immediately follows insert)
Proper citation: RRID:Addgene_82783 Copy
Species: Homo sapiens
Genetic Insert: KEAP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27478040
Comments: CLOSED (stop codon immediately follows insert)
Proper citation: RRID:Addgene_82781 Copy
Species: Homo sapiens
Genetic Insert: CCND1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27478040
Comments: CLOSED (stop codon immediately follows insert)
Proper citation: RRID:Addgene_82733 Copy
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