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Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376751
Comments: This plasmid contains a wild-type loxP site and a mutant loxP site termed loxM3. Addgene's mapping software can not differentiate between the two sites and identifies the loxM3 site as loxP. The locations of the loxP and loxM3 sites are:
loxP = 3859-3892
loxM3 = 4141-4174
Proper citation: RRID:Addgene_48983 Copy
Vector Backbone Description: Backbone Size:2492; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Gentamicin
Defining Citation: PMID:24376629
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 602-612bp
BsaI site #2 = 2040-2050bp
Proper citation: RRID:Addgene_48867 Copy
Genetic Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Vector Backbone Description: Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin
Comments: strain contains pZE41 backbone (specR), fadD, tesA'
galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Proper citation: RRID:Addgene_48900 Copy
Vector Backbone Description: Backbone Size:2492; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Gentamicin
Defining Citation: PMID:24376629
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Please note that a transposon insertion is present in the chloramphenicol resistance promoter. This insertion does not interfere with plasmid function. There is an additional BsaI site at bp# 1889-1894 which is irrelevant for GreenGate reactions with this plasmid.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 640-650bp
transposon integration = 747-2077bp
BsaI site #2 = 3414-3419bp
Proper citation: RRID:Addgene_48866 Copy
Genetic Insert: blasticidin-S deaminase
Vector Backbone Description: Backbone Marker:Dankort Lab; Vector Backbone:pBEG R2+xL3 (Addgene plasmid 48952); Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48982 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376751
Comments: This plasmid contains a wild-type loxP site and a mutant loxP site termed loxM3. Addgene's mapping software can not differentiate between the two sites and identifies the loxM3 site as loxP. The locations of the loxP and loxM3 sites are:
loxP = 3859-3892
loxM3 = 4074-4107
Proper citation: RRID:Addgene_48981 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376751
Comments: This plasmid contains a wild-type loxP site and a mutant loxP site termed loxM3. Addgene's mapping software can not differentiate between the two sites and identifies the loxM3 site as loxP. The locations of the loxP and loxM3 sites are:
loxP = 3859-3892
loxM3 = 3987-4020
Proper citation: RRID:Addgene_48975 Copy
Vector Backbone Description: Backbone Marker:Malcolm Moore lab; Vector Backbone:pUltra; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: AmCyan1 excitation and emission maxima are 458 and 489 nm, respectively
Proper citation: RRID:Addgene_48974 Copy
Species: Synthetic
Genetic Insert: 3xFNLDD
Vector Backbone Description: Backbone Marker:Toshio Kitamura; Backbone Size:5994; Vector Backbone:pMXs-IG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25607658
Proper citation: RRID:Addgene_48972 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376751
Comments: This plasmid contains a wild-type loxP site and a mutant loxP site termed loxM3. Addgene's mapping software can not differentiate between the two sites and identifies the loxM3 site as loxP. The locations of the loxP and loxM3 sites are:
loxP = 3859-3892
loxM3 = 4014-4047
Proper citation: RRID:Addgene_48976 Copy
Species: Homo sapiens
Genetic Insert: MALT1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22309193
Proper citation: RRID:Addgene_48971 Copy
Species: Homo sapiens
Genetic Insert: SUMO3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48964 Copy
Species: Homo sapiens
Genetic Insert: SUMO3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48963 Copy
Vector Backbone Description: Backbone Marker:life technologies; Vector Backbone:pMA-T; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013562
Proper citation: RRID:Addgene_48961 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Comments: Please note this plasmid contains the mature form of SUMO1, which lacks the last 4 amino acids from the C-terminus.
Proper citation: RRID:Addgene_48966 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48965 Copy
Genetic Insert: cas9
Vector Backbone Description: Backbone Marker:life technologies; Backbone Size:4959; Vector Backbone:pDEST; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013562
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_48960 Copy
Vector Backbone Description: Backbone Marker:Dankort lab, unpublished; Backbone Size:7396; Vector Backbone:gQxiPuro; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48958 Copy
Vector Backbone Description: Backbone Marker:Messing Lab, PMID 1905257; Backbone Size:3772; Vector Backbone:pOK1/2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48942 Copy
Genetic Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Vector Backbone Description: Vector Backbone:pZE41 backbone (specR), fadD, tesA',fadR; Vector Types:; Bacterial Resistance:Spectinomycin
Comments: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
fadE- fadR->CAT, plTet0-> (tesA and fadD), fadR overexpression
Proper citation: RRID:Addgene_48939 Copy
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