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  • Bacterial Resistance:spectinomycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pUB-spHRed
 
Resource Report
Resource Website
RRID:Addgene_84734 pHRed Other Spectinomycin PMID:28663550 Backbone Marker:Grefen et al. The Plant Journal (2010) 64, 355–365; Backbone Size:9210; Vector Backbone:pUB-DEST; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:04 0
TF101-STTM-miR408
 
Resource Report
Resource Website
RRID:Addgene_84814 STTM-miR408 Synthetic Spectinomycin PMID:23098881 Backbone Marker:Dr. Kang Wang at Iowa State University; Vector Backbone:TF101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:04 0
TF101-STTM-miR394
 
Resource Report
Resource Website
RRID:Addgene_84813 STTM-miR394 Synthetic Spectinomycin PMID:23098881 Please note that bp8 of the provided STTM-miR394 sequence is missing. The depositing lab noted that this missing bp should not affect plasmid function. Backbone Marker:Dr. Kang Wang at Iowa State University; Vector Backbone:TF101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin bp8 is deleted- please see depositor comments below 2026-08-29 01:11:56 0
TF101-STTM-miR165/166
 
Resource Report
Resource Website
RRID:Addgene_84816 STTM-miR165/166 Synthetic Spectinomycin PMID:23098881 Backbone Marker:Dr. Kang Wang at Iowa State University; Vector Backbone:TF101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:11:56 0
TF101-STTM-miR172
 
Resource Report
Resource Website
RRID:Addgene_84815 STTM-miR172 Synthetic Spectinomycin PMID:23098881 Backbone Marker:Dr. Kang Wang at Iowa State University; Vector Backbone:TF101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:11:56 0
Bsrs078-RpaR
 
Resource Report
Resource Website
RRID:Addgene_85148 RpaR Spectinomycin PMID:27172092 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Vector Backbone:pBjk2807; Vector Types:; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:06 0
Bsrs078-LasR
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_85147 LasR Spectinomycin PMID:27172092 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Vector Backbone:pBjk2807; Vector Types:; Bacterial Resistance:Spectinomycin 2026-08-29 01:11:59 1
Bsrs078-TraR
 
Resource Report
Resource Website
RRID:Addgene_85150 TraR Spectinomycin PMID:27172092 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Vector Backbone:pBjk2807; Vector Types:; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:06 0
pAM5085
 
Resource Report
Resource Website
RRID:Addgene_85122 PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly. Synechoccus elongatus PCC 7942 Spectinomycin PMID:25127213 Vector Backbone:PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:11:59 0
pJYS2_crtYf
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_85544 crRNA of crtYf Synthetic Spectinomycin PMID:28469274 Vector Backbone:pXMJ19 and pTRCmob; Vector Types:Bacterial Expression, CRISPR, Shuttle vector Corynebacterium glutamicum / E. coli; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:03 3
pDule2-pCNF
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_85495 para-cyanophenylalanine Mj synthetase Methanocaldococcus jannaschii Spectinomycin PMID:19492814 Backbone Size:5400; Vector Backbone:pDule2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin Y32L L65V F108W Q109M D158G I159P 2026-08-29 01:12:02 5
pDule2-Tet2.0
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_85497 Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system Methanocaldococcus jannaschii Spectinomycin PMID:26237426 Backbone Size:5400; Vector Backbone:pDule2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin Y32G L65Q F108S Q109D D158S L162N 2026-08-29 01:12:02 1
pDule2-para-aminoPhe
 
Resource Report
Resource Website
RRID:Addgene_85503 p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system Methanocaldococcus jannaschii Spectinomycin PMID:12537491 Backbone Size:5400; Vector Backbone:pDule2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin Y32T, E107T, D158P, I159L, L162A 2026-08-29 01:12:02 0
pDule2-IBBN (G2)
 
Resource Report
Resource Website
RRID:Addgene_85501 IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system Methanocaldococcus jannaschii Spectinomycin PMID:20839808 This synthetase is permissive and can incorporate a variety of amino acids structurally analogous to IBBN. For a more complete functional and structural analysis of this synthetase, also see Cooley et al. Chembiochem. 2014, 15(12): 1810–1819. Backbone Size:5400; Vector Backbone:pDule2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin Y32G L65E F108W Q109M D158S R162K 2026-08-29 01:12:02 0
pPEPX-P3-sgRNAluc
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_85590 sgRNA targeting firefly luciferase encoding gene Synthetic Spectinomycin PMID:28490437 For information of vector pPEP1 and promoter of P3, please refer to "Sorg RA, Kuipers OP, Veening JW (2014) Gene Expression Platform for Synthetic Biology in the Human Pathogen Streptococcus pneumoniae. ACS synthetic biology" Backbone Size:3245; Vector Backbone:pPEP1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:03 1
pKI1.1R
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_85808 human-codon-optimized SpCas9 Streptococcus pyogenes Spectinomycin PMID:27856772 Although this vector does not has a problem of sgRNA truncation like pKIR1.1, cloning efficiency in vector construction is low. E. coli with high competency is required. Backbone Marker:Prof. Ikuko Hara-Nishimura; Vector Backbone:pFAST-R01; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:08 10
pKIR1.1
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_85758 human-codon-optimized SpCas9 Streptococcus pyogenes Spectinomycin PMID:27856772 Right border (RB) may be truncated in the T-DNA integration. This may result in sgRNA scaffold truncation because the RB is close to 3' end of sgRNA. However, since we efficiently obtained knockout mutants by this vector, the truncation is not serious problem. To overcome it, we also deposited pKI1.1R. In pKI1.1R, sgRNA is not close to RB. Backbone Marker:Prof. Ikuko Hara-Nishimura; Vector Backbone:pFAST-R01; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:08 3
pDONR223_ZBTB24_p.G568C
 
Resource Report
Resource Website
RRID:Addgene_82783 ZBTB24 Homo sapiens Spectinomycin PMID:27478040 CLOSED (stop codon immediately follows insert) Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin G568C 2026-08-29 01:11:36 0
pDONR223_KEAP1_p.R470S
 
Resource Report
Resource Website
RRID:Addgene_82781 KEAP1 Homo sapiens Spectinomycin PMID:27478040 CLOSED (stop codon immediately follows insert) Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin R470S; C624S 2026-08-29 01:11:44 0
pDONR223_CCND1_p.E172K
 
Resource Report
Resource Website
RRID:Addgene_82733 CCND1 Homo sapiens Spectinomycin PMID:27478040 CLOSED (stop codon immediately follows insert) Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin E172K 2026-08-29 01:11:36 0

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