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Species: Synthetic
Genetic Insert: tdTomato sgRNA
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang Lab; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35789697
Comments: This plasmid was cloned with the one step BbsI restriction enzyme cloning method. Please note that gRNA sequences that do not start with a G, need a G added to the sequence before cloning for U6 promoter transcription.
Proper citation: RRID:Addgene_179919 Copy
Species: Synthetic
Genetic Insert: CMV promoter sgRNA
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang Lab; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35789697
Comments: This plasmid was cloned with the one step BbsI restriction enzyme cloning method. Please note that gRNA sequences that do not start with a G, need a G added to the sequence before cloning for U6 promoter transcription.
Proper citation: RRID:Addgene_179917 Copy
Species: Synthetic
Genetic Insert: GBP nanobody
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pUASp-attB-K10; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35020877
Proper citation: RRID:Addgene_179995 Copy
Species: Synthetic
Genetic Insert: GBP nanobody
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pUASt-attB-K10; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35020877
Proper citation: RRID:Addgene_179996 Copy
Species: Drosophila melanogaster
Genetic Insert: Egl-Trbo
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pUASp-attB-K10; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35020877
Proper citation: RRID:Addgene_179993 Copy
Species: Homo sapiens
Genetic Insert: WW Domain Containing Transcription Regulator 1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34464659
Proper citation: RRID:Addgene_179911 Copy
Species: Homo sapiens
Genetic Insert: WW Domain Containing Transcription Regulator 1
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pT3-EF1aH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34464659
Proper citation: RRID:Addgene_179910 Copy
Species: Synthetic
Genetic Insert: Kir2.1
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179707 Copy
Species: Synthetic
Genetic Insert: TrpLE-M-nMSP1E3D1
Vector Backbone Description: Backbone Marker:Gene Universal; Backbone Size:5310; Vector Backbone:pET-24a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34848201
Proper citation: RRID:Addgene_179949 Copy
Species: Synthetic
Genetic Insert: Kir2.1
Vector Backbone Description: Vector Backbone:CMVTO-FlpIn; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35177637
Proper citation: RRID:Addgene_179705 Copy
Species: Homo sapiens
Genetic Insert: Wnt-3
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Roelink, H., and Nusse, R. Expression of two members of the Wnt family during mouse development -restricted temporal and spatial patterns in the developing neural tube. Genes Dev., 5: 381-388, 1991.
Proper citation: RRID:Addgene_17993 Copy
Species: Mus musculus
Genetic Insert: Histone H3
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://omu.repo.nii.ac.jp/records/718 for the article and updated figures.
Please visit https://www.hilarispublisher.com/open-access/molecular-behavior-of-cenpa-and-pcna-throughout-the-cell-cycle-inliving-human-ht1080-cells-2168-9547-1000162.pdf to access the original publication.
Plasmid pmPlum-C1 was constructed by inserting the amplified DNA fragment encoding mPlum of pBAD-mPlum.
For more information, please also see Tanaka et al. (2017) bioimages. https://doi.org/10.11169/bioimages.25.7
Proper citation: RRID:Addgene_179700 Copy
Species: Mus musculus
Genetic Insert: Wnt-3a
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Proper citation: RRID:Addgene_17994 Copy
Species: Caenorhabditis elegans
Genetic Insert: NLF-1::GFP
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7441; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23522043
Comments: Please note Addgene NGS identified 3 mutations in the exons- I51N (exon1), T39A, L233S (exon2). Depositor confirms mutations do not affect plasmid function.
Proper citation: RRID:Addgene_179773 Copy
Species: Caenorhabditis elegans
Genetic Insert: NLF-1::2XGFP
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5089; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23522043
Comments: Please note Addgene NGS found some differences in genomic sequence of NLF-1. Depositor confirms this does not affect plasmid function.
Proper citation: RRID:Addgene_179771 Copy
Species: Acanthoeca spectabilis
Genetic Insert: As-NF-kB1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916615
Comments: Codon optimized for human cell expression
Proper citation: RRID:Addgene_180203 Copy
Species: E. coli
Genetic Insert: FLIPglu F16F Delta13
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5603; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20354141
Proper citation: RRID:Addgene_18022 Copy
Species: Mus musculus
Genetic Insert: Wnt-6
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Proper citation: RRID:Addgene_18024 Copy
Species: Mus musculus
Genetic Insert: Wnt-7b
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Proper citation: RRID:Addgene_18026 Copy
Species: Neisseria lactamica
Genetic Insert: NlaCas3c
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5203; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_180214 Copy
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