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Species: Plasmodium ovale
Genetic Insert: MSP10
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126831 Copy
Species: Plasmodium ovale
Genetic Insert: P41
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126836 Copy
Species: Plasmodium ovale
Genetic Insert: MSP3.5
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126837 Copy
Species: Plasmodium ovale
Genetic Insert: P12
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126834 Copy
Species: Plasmodium ovale
Genetic Insert: P38
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126835 Copy
Species: Plasmodium malariae
Genetic Insert: MSP7.1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126827 Copy
Species: Plasmodium malariae
Genetic Insert: MSP7.4
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126828 Copy
Species: Plasmodium malariae
Genetic Insert: MSP5
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126821 Copy
Species: Plasmodium malariae
Genetic Insert: MSP4
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126820 Copy
Species: Plasmodium malariae
Genetic Insert: MSP3.5
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126825 Copy
Species: Plasmodium malariae
Genetic Insert: P41
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6688; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31952523
Proper citation: RRID:Addgene_126824 Copy
Species: S. pyogenes
Genetic Insert: SpCas9-HF1
Vector Backbone Description: Vector Backbone:pMJ806-like; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: pET-T7-6xHis-MBP-TEV-FLAG-NLS-SpCas9-HF1-NLS
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126770 Copy
Species: S. pyogenes
Genetic Insert: eSpCas9-plus
Vector Backbone Description: Vector Backbone:pMJ806-like; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: pET-T7-6xHis-MBP-TEV-FLAG-NLS-eSpCas9-plus-NLS
eSpCas9-plus cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs. Shows almost the same fidelity and target selectivity as its parental increased fidelity variant, eSpCas9.
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126774 Copy
Species: S. pyogenes
Genetic Insert: B-eSpCas9
Vector Backbone Description: Vector Backbone:pMJ806-like; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: pET-T7-6xHis-MBP-TEV-FLAG-NLS-Blackjack-eSpCas9-NLS
Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-eSpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, eSpCas9.
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126772 Copy
Species: S. pyogenes
Genetic Insert: Sniper SpCas9
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32144253
Comments: pCbh-3xFLAG-NLS-Sniper SpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations).
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126777 Copy
Species: S. pyogenes
Genetic Insert: HiFi SpCas9
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32144253
Comments: pCbh-3xFLAG-NLS-HiFi SpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations).
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126778 Copy
Species: Other
Genetic Insert: monomeric filamin-mEos3.2
Vector Backbone Description: Vector Backbone:pDXA-HY; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126811 Copy
Species: S. pyogenes
Genetic Insert: SpCas9-HF1-plus
Vector Backbone Description: Vector Backbone:pMJ806-like; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: pET-T7-6xHis-MBP-TEV-FLAG-NLS-SpCas9-HF1-plus-NLS
SpCas9-HF1-plus cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs. Shows almost the same fidelity and target selectivity as its parental increased fidelity variant, SpCas9-HF1.
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126775 Copy
Species: S. pyogenes
Genetic Insert: sgRNA (for SpCas9)
Vector Backbone Description: Vector Backbone:pmCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32144253
Comments: gRNA cloning vector (U6_BbsI sites_sgRNA) with mCherry transfection control (CMV_mCherry cassette), lacks the direct repeat (a truncated guideRNA) after the 3'-end of the sgRNA.
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126776 Copy
Species: Other
Genetic Insert: Filamin-mRFP1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126814 Copy
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