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Genetic Insert: Empty vector
Vector Backbone Description: Backbone Marker:Bill Rutter; Backbone Size:2924; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3518947
Comments: Plasmid kindly provided to Addgene by Dr. Anne Brunet.
There are a few single nucleotide differences and one small insertion relative to author sequence but they do not alter the MCS or affect plasmid function.
Proper citation: RRID:Addgene_26453 Copy
Species: Mus musculus
Genetic Insert: cytoplasmic dynein 1 intermediate chain 1 isoform D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:subcloning and sequencing; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20657784
Proper citation: RRID:Addgene_26439 Copy
Genetic Insert: pT/BH
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2914; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: There are three mismatches between Addgene and author sequence but they are in vector backbone.
Proper citation: RRID:Addgene_26554 Copy
Genetic Insert: pT2/SVNeo
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3712; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12083513
Proper citation: RRID:Addgene_26553 Copy
Species: Homo sapiens
Genetic Insert: miR-155
Vector Backbone Description: Backbone Marker:Addgene plasmid 12282; Backbone Size:6500; Vector Backbone:pMIG-w; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18299402
Comments: MG155 was derived originally from pMIG-w. The GFP and IRES were removed using BglII and HindIII, and a new cassette with eGFP (about 700 bps) followed by a miR-155 sequence (about 500 bps with flanking Not1 and XhoI sites) was cloned in using BamHI (destroying the BglII site) and HindIII. This results in a 7 kb plasmid.
Proper citation: RRID:Addgene_26529 Copy
Species: S. mediterranea (planarian)
Genetic Insert: ilp-1
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript SKII (+); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20967238
Comments: Addgene's quality control sequence has minor discrepancies with the provided depositor's sequence; these do not affect plasmid function.
Proper citation: RRID:Addgene_26522 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7 hairpin only
Vector Backbone Description: Backbone Size:6946; Vector Backbone:MDH1-PGK-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26488 Copy
Species: Mus musculus
Genetic Insert: Osr1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10473132
Proper citation: RRID:Addgene_26485 Copy
Species: S. mediterranea (planarian)
Genetic Insert: npp-4
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26484 Copy
Species: S. mediterranea (planarian)
Genetic Insert: ppp-1
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26481 Copy
Species: S. mediterranea (planarian)
Genetic Insert: npy-1
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26480 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-15
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
The insert has minor sequence discrepancies with NCBI; these do not affect plasmid function.
Proper citation: RRID:Addgene_26483 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-11
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26482 Copy
Species: Homo sapiens
Genetic Insert: IL7
Vector Backbone Description: Backbone Size:7707; Vector Backbone:pUC18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_26532 Copy
Species: S. mediterranea (planarian)
Genetic Insert: npp-8
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Proper citation: RRID:Addgene_26535 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-5
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Proper citation: RRID:Addgene_26534 Copy
Species: Mus musculus
Genetic Insert: SHIP1 3'UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pmiReport; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19359473
Comments: The inserts put into this vector are downstream from luciferase. They create different 3' UTRs for luciferase.
Proper citation: RRID:Addgene_26531 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-17
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: Addgene's quality control sequence has minor discrepancies with the provided depositor's sequence; these do not affect plasmid function.
Proper citation: RRID:Addgene_26497 Copy
Species: Mus musculus
Genetic Insert: PU.1 3' UTR
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pmiReport; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18299402
Comments: The inserts put into this vector are downstream from luciferase. They create different 3' UTRs for luciferase.
Proper citation: RRID:Addgene_26530 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7 (~250nt flanking each side)
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7060; Vector Backbone:MXW-PGK-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: Ordinarily, we express miRNAs from the LTR, which is pol III. However, cel-let-7 won't express from that promoter (possibly due to long poly-T tracts). This is a pol II expression system.
Proper citation: RRID:Addgene_26491 Copy
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