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Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403704
Comments: Allen Institute plasmid ALIAS: AiP14948 This work supported by NIH BRAIN Initiative grant UF1MH128339
Proper citation: RRID:Addgene_214867 Copy
Species: Synthetic
Genetic Insert: iCre(R297T)
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403704
Comments: Allen Institute plasmid ALIAS: AiP15138 This work supported by NIH BRAIN Initiative grant UF1MH128339
Proper citation: RRID:Addgene_214868 Copy
Species: Synthetic
Genetic Insert: CoChR-EGFP
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403704
Comments: Allen Institute plasmid ALIAS: AiP15140 This work supported by NIH BRAIN Initiative grant UF1MH128339
Proper citation: RRID:Addgene_214869 Copy
Species: Synthetic
Genetic Insert: ChR2(H134R)-EYFP
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403704
Comments: Allen Institute plasmid ALIAS: AiP14823 This work supported by NIH BRAIN Initiative grant UF1MH128339
Proper citation: RRID:Addgene_214860 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:6000; Vector Backbone:BBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37988619
Proper citation: RRID:Addgene_215310 Copy
Species: Synthetic
Genetic Insert: iCre(R297T)
Vector Backbone Description: Vector Backbone:rAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40403704
Comments: Allen Institute plasmid ALIAS: AiP14825 This work supported by NIH BRAIN Initiative grant UF1MH128339
Proper citation: RRID:Addgene_214862 Copy
Species: Synthetic
Genetic Insert: WT-eGFP
Vector Backbone Description: Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_215168 Copy
Species: Synthetic
Genetic Insert: His-GST
Vector Backbone Description: Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_215169 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:4300; Vector Backbone:BBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37988619
Proper citation: RRID:Addgene_215337 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:BBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37988619
Proper citation: RRID:Addgene_215341 Copy
Species: Synthetic
Genetic Insert: S. stutzeri nifL gRNA
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37988619
Proper citation: RRID:Addgene_215343 Copy
Species: Synthetic
Genetic Insert: 35s:P19:tNos
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_alpha2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37882352
Proper citation: RRID:Addgene_215369 Copy
Species: Synthetic
Genetic Insert: MGGAGM Linker
Vector Backbone Description: Vector Backbone:pGWB502-omega; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39100877
Comments: Please visit https://www.biorxiv.org/content/10.1101/2024.06.28.601222v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_216141 Copy
Species: Synthetic
Genetic Insert: 3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
Vector Backbone Description: Backbone Marker:Dr. Peter Jan Vonk; Backbone Size:7568; Vector Backbone:pPV033; Vector Types:Fungal homologous recombination plasmid; Bacterial Resistance:Ampicillin
Comments: Use BcuI (SpeI) site to integrate your construct of interest (e.g. promoter + gene) upstream of the HA-tag for integration of the construct at the targeted integration locus in S. commune. Use this approach to complement a gene knockout, combined with the possibility to subsequently perform pull-down experiments like Western Blotting or ChIP-Seq.
Proper citation: RRID:Addgene_215568 Copy
Species: Synthetic
Genetic Insert: Tras heavy chain-SpyTag003
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38493197
Proper citation: RRID:Addgene_216293 Copy
Species: Synthetic
Genetic Insert: Tras heavy chain-GSG-SpyTag003
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38493197
Proper citation: RRID:Addgene_216292 Copy
Species: Synthetic
Genetic Insert: SpyCatcher002-MBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38493197
Proper citation: RRID:Addgene_216295 Copy
Species: Synthetic
Genetic Insert: DoubleCatcher ⍺-Lock
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:6422; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38493197
Proper citation: RRID:Addgene_216286 Copy
Species: Synthetic
Genetic Insert: MF3958 Light chain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38493197
Proper citation: RRID:Addgene_216304 Copy
Species: Synthetic
Genetic Insert: H2-18 Light chain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38493197
Proper citation: RRID:Addgene_216308 Copy
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