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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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AICSDP-35:AAVS1-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_91565 | AAVS1 Homology Arms with CAGGS driven mEGFP | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to insert a CAGGS-mEGFP at the AAVS1 safe harbor locus in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After insertion of the reporter using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:unknown; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | 2026-09-01 10:05:17 | 3 | ||
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LZ10 PBREBAC-H2BHalo Resource Report Resource Website 1+ mentions |
RRID:Addgene_91564 | histone H2B-HaloTag | Homo sapiens | Ampicillin | PMID:27484239 | Backbone Marker:synthetic; Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 10:05:17 | 8 | ||
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pSAM_Cam Resource Report Resource Website 1+ mentions |
RRID:Addgene_91570 | Chloramphenicol and Kanamycin | PMID:26060277 | Mariner himar1C9 transposase driven by lac promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Chloramphenicol resistance is contained on the plasmid backbone. For further background, see: Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017. Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017. | Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin | 2026-09-01 10:05:17 | 1 | |||
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7TFP CDH1 mutant reporter Resource Report Resource Website 1+ mentions |
RRID:Addgene_91703 | CDH1 mutant promoter | Homo sapiens | Ampicillin | PMID:26941323 | Backbone Marker:Roel Nusse (Addgene plasmid # 24308); Backbone Size:9061; Vector Backbone:7TFP; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin | mutant E-boxes | 2026-09-01 10:05:19 | 1 | |
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7TFP CDH1 reporter Resource Report Resource Website 1+ mentions |
RRID:Addgene_91704 | CDH1 promoter | Homo sapiens | Ampicillin | PMID:26941323 | Backbone Marker:Roel Nusse (Addgene plasmid # 24308); Backbone Size:9061; Vector Backbone:7TFP; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin | 2026-09-01 10:05:19 | 4 | ||
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pLIX402 caPHF2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_91701 | PHF2 | Homo sapiens | Ampicillin | PMID:26941323 | Backbone Marker:David Root (Addgene plasmid # 41394); Backbone Size:9394; Vector Backbone:pLIX402; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | caPHF2; S757D, S899D, S954D, S1056D | 2026-09-01 10:05:19 | 1 | |
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pLIX402 PHF2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_91700 | PHF2 | Homo sapiens | Ampicillin | PMID:26941323 | Backbone Marker:David Root (Addgene plasmid # 41394); Backbone Size:9394; Vector Backbone:pLIX402; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 10:05:19 | 1 | ||
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pRRlsinPGK_H2BGFP_WPRE Resource Report Resource Website 1+ mentions |
RRID:Addgene_91788 | Human histone H2B | Homo sapiens | Ampicillin | Backbone Marker:Trono lab; Backbone Size:7840; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 10:05:20 | 2 | |||
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pRRlsinPGK_dectintagBFP2_WPRE Resource Report Resource Website 1+ mentions |
RRID:Addgene_91786 | Dectin1A | Homo sapiens | Ampicillin | Backbone Marker:Trono lab; Backbone Size:8179; Vector Backbone:pRRL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 10:05:20 | 1 | |||
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pGP-CMV-GCaMP3-373 Resource Report Resource Website 1+ mentions |
RRID:Addgene_91779 | GCaMP3-373 | Rattus norvegicus | Kanamycin | PMID:28162809 | Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-01 10:05:20 | 1 | ||
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pGP-CMV-GCaMP6-150 Resource Report Resource Website 1+ mentions |
RRID:Addgene_91777 | GCaMP6-150 | Rattus norvegicus | Kanamycin | PMID:28162809 | Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-01 10:05:20 | 2 | ||
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CaMKIIP-mEGFP-P2A-paAIP2/pAAV Resource Report Resource Website 1+ mentions |
RRID:Addgene_91718 | mEGFP-P2A-paAIP2 | Synthetic | Ampicillin | PMID:28318784 | Backbone Size:4650; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2026-09-01 10:05:19 | 2 | ||
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Hy_pMT Laccase2 Exons 1-3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_91799 | Laccase2 | Drosophila melanogaster | Ampicillin | PMID:29174924 | Vector Backbone:Hy_pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-09-01 10:05:20 | 1 | ||
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pCRIS-PITChv2-dTAG-Puro (BRD4) Resource Report Resource Website 10+ mentions |
RRID:Addgene_91796 | FKBP_F36V-2xHA-P2A-Puro | Synthetic | Ampicillin | PMID:29581585 | See: Sakuma, T., Nakade, S., Sakane, Y., Suzuki, K.-I. & Yamamoto, T. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc 11, 118–133 (2016). for the original backbone and general cloning protocol. | Backbone Marker:Takashi Yamamoto lab; Backbone Size:4579; Vector Backbone:pCRIS-PITChv2 (addgene #63672); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | Phenylalanine 36 to valine in FKBP12 | 2026-09-01 10:05:20 | 14 |
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pLEX_305-N-dTAG Resource Report Resource Website 10+ mentions |
RRID:Addgene_91797 | Chloramphenicol and Ampicillin | PMID:29581585 | Backbone Marker:David Root Lab (addgene #41390); Backbone Size:9621; Vector Backbone:pLEX_305; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-09-01 10:05:20 | 20 | ||||
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pCRIS-PITChv2-dTAG-BSD (BRD4) Resource Report Resource Website 10+ mentions |
RRID:Addgene_91795 | FKBP_F36V-2xHA-P2A-BSD | Synthetic | Ampicillin | PMID:29581585 | See: Sakuma, T., Nakade, S., Sakane, Y., Suzuki, K.-I. & Yamamoto, T. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc 11, 118–133 (2016). for the original backbone and general cloning protocol. | Backbone Marker:Takashi Yamamoto lab; Backbone Size:4579; Vector Backbone:pCRIS-PITChv2 (addgene #63672); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | Phenylalanine 36 to valine in FKBP12 | 2026-09-01 10:05:20 | 11 |
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pCRIS-PITChv2-Puro-dTAG (BRD4) Resource Report Resource Website 10+ mentions |
RRID:Addgene_91793 | Puro-P2A-2xHA-FKBP_F36V | Synthetic | Ampicillin | PMID:29581585 | See: Sakuma, T., Nakade, S., Sakane, Y., Suzuki, K.-I. & Yamamoto, T. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc 11, 118–133 (2016). for the original backbone and general cloning protocol. | Backbone Marker:Takashi Yamamoto lab; Backbone Size:4579; Vector Backbone:pCRIS-PITChv2 (addgene #63672); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | Phenylalanine 36 to valine in FKBP12 | 2026-09-01 10:05:20 | 19 |
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pDicAID_nCas9-PmCDA-2A-NptII_ETR Resource Report Resource Website 1+ mentions |
RRID:Addgene_91695 | SpCas9 | Streptococcus pyogenes | Spectinomycin | PMID:28346401 | Backbone Size:17766; Vector Backbone:pZD_OsU3gYSA_HolgerCas9_NPTII; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | D10A for nickase Cas9 | 2026-09-01 10:05:19 | 2 | |
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pNCS mCherry Resource Report Resource Website 1+ mentions |
RRID:Addgene_91769 | monomeric Cherry | Discosoma sp. (mushroom coral) | Ampicillin | PMID:15558047 | These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork. Addgene next-generation sequencing identified a deletion of Glutimate and Glycine residues (amino acids 73 and 74 relative to Genbank ID: 6MZ3_A). The depositing laboratory has observed expression of the chromophore in E. coli. | Backbone Marker:Life Technologies; Backbone Size:2900; Vector Backbone:modified pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | See Depositor Comments | 2026-09-01 10:05:20 | 1 |
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pNCS tdTomato Resource Report Resource Website 1+ mentions |
RRID:Addgene_91767 | tandem dimer Tomato | Discosoma sp. (mushroom coral) | Ampicillin | PMID:15558047 | These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork. | Backbone Marker:Life Technologies; Backbone Size:2900; Vector Backbone:modified pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-01 10:05:20 | 2 |
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