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Species: E. coli
Genetic Insert: FLIPglu D154A Delta13
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20354141
Proper citation: RRID:Addgene_18015 Copy
Species: Homo sapiens
Genetic Insert: human GPIbalpha 1-290aa with mutation G233V and M239V
Vector Backbone Description: Backbone Size:5341; Vector Backbone:ET8; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30797858
Proper citation: RRID:Addgene_180251 Copy
Species: P. berghei
Genetic Insert: D3 of P. berghei HAP2 with N-glycosylation sites mutated
Vector Backbone Description: Vector Backbone:pD2529CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34939934
Proper citation: RRID:Addgene_180252 Copy
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35145312
Comments: Digest vector with AgeI+NheI to clone any sequence between the ribozymes
Proper citation: RRID:Addgene_180184 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike A570D
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180064 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike K417N
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180060 Copy
Species: Danio rerio
Genetic Insert: Histone deacetylase 6
Vector Backbone Description: Vector Backbone:pAC8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36255643
Proper citation: RRID:Addgene_180226 Copy
Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_180228 Copy
Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_180229 Copy
Species: Capsaspora owczarzaki
Genetic Insert: Co-NF-kB
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916615
Comments: Codon optimized for human cell expression
Proper citation: RRID:Addgene_180197 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike L241-L242-A243-
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180070 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 N S235F
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180071 Copy
Species: Capsaspora owczarzaki
Genetic Insert: Co-NF-kBCterm
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916615
Comments: Codon optimized for human cell expression
Proper citation: RRID:Addgene_180199 Copy
Genetic Insert: CasMINI sgRNA (backbone) and BFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34480847
Proper citation: RRID:Addgene_180280 Copy
Species: Mus musculus
Genetic Insert: Wnt-4HA
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Proper citation: RRID:Addgene_18031 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*24:02
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00050 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180445 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*08:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00146 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180447 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*01:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00001 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180448 Copy
Species: Macaca mulatta (Rhesus Macaque)
Genetic Insert: Mamu-A1*002:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET23a+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: https://www.ebi.ac.uk/ipd/mhc/allele/?accession=NHP01866 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180443 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*35:01
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00237 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180450 Copy
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