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Species: Other
Genetic Insert: filamin deltaABD-mEos3.2
Vector Backbone Description: Vector Backbone:pDXA-HY; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126812 Copy
Species: Other
Genetic Insert: filamin-mEos2
Vector Backbone Description: Vector Backbone:pDXA-HY; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126807 Copy
Species: Other
Genetic Insert: mEos2
Vector Backbone Description: Vector Backbone:pDXA-HY; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126805 Copy
Species: S. pyogenes
Genetic Insert: B-HypaSpCas9
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32144253
Comments: pCbh-3xFLAG-NLS-Blackjack-HypaSpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations).
Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-HypaSpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, HypaSpCas9.
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126763 Copy
Species: S. pyogenes
Genetic Insert: B-HeFSpCas9
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32144253
Comments: pCbh-3xFLAG-NLS-Blackjack-HeFSpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations).
Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-HeFSpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, HeFSpCas9.
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126766 Copy
Species: S. pyogenes
Genetic Insert: B-evoSpCas9
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32144253
Comments: pCbh-3xFLAG-NLS-Blackjack-evoSpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations).
Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-evoSpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, evoSpCas9.
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126765 Copy
Species: S. pyogenes
Genetic Insert: SpCas9-HF1-plus
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32144253
Comments: pCbh-3xFLAG-NLS-SpCas9-HF1-plus-NLS (without U6-sgRNA coding sequence, with silent mutations).
SpCas9-HF1-plus cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs. Shows almost the same fidelity and target selectivity as its parental increased fidelity variant, SpCas9-HF1.
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication.
Proper citation: RRID:Addgene_126768 Copy
Species: Xanthophyllomyces dendrorhous
Genetic Insert: XdCrtE
Vector Backbone Description: Vector Backbone:pCfB6677; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31556952
Proper citation: RRID:Addgene_126918 Copy
Species: Xanthophyllomyces dendrorhous
Genetic Insert: XdCrtI
Vector Backbone Description: Vector Backbone:pCfB2904; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31556952
Proper citation: RRID:Addgene_126916 Copy
Genetic Insert: SurE
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET-21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11524683
Comments: Note: The SurE insert contains an additional mutation (Q12R) that the depositor has confirmed does not affect plasmid function.
Proper citation: RRID:Addgene_12686 Copy
Species: Homo sapiens
Genetic Insert: GAR1
Vector Backbone Description: Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20351177
Proper citation: RRID:Addgene_126873 Copy
Species: Other
Genetic Insert: IDA3
Vector Backbone Description: Vector Backbone:pHyg3; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29467251
Proper citation: RRID:Addgene_126876 Copy
Vector Backbone Description: Vector Backbone:pCDNA5/to; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_126877 Copy
Species: Other
Genetic Insert: IDA3
Vector Backbone Description: Vector Backbone:pHyg3; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29467251
Proper citation: RRID:Addgene_126874 Copy
Species: Other
Genetic Insert: IDA3
Vector Backbone Description: Vector Backbone:pHyg3; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29467251
Proper citation: RRID:Addgene_126875 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: X-4 and XII-5 gRNA
Vector Backbone Description: Vector Backbone:pTAJAK-71; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31556952
Proper citation: RRID:Addgene_126911 Copy
Species: Homo sapiens
Genetic Insert: Human Cdc45
Vector Backbone Description: Backbone Size:3766; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27189187
Proper citation: RRID:Addgene_126879 Copy
Species: Xanthophyllomyces dendrorhous
Genetic Insert: XdCrtI
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31556952
Proper citation: RRID:Addgene_126906 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126904 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12694 Copy
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