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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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FLN-deltaABD-mEos3.2 Resource Report Resource Website |
RRID:Addgene_126812 | filamin deltaABD-mEos3.2 | Other | Ampicillin | Vector Backbone:pDXA-HY; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:50 | 0 | |||
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FLN-mEos2 Resource Report Resource Website |
RRID:Addgene_126807 | filamin-mEos2 | Other | Ampicillin | Vector Backbone:pDXA-HY; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:50 | 0 | |||
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pDXA-HY-mEos2 Resource Report Resource Website |
RRID:Addgene_126805 | mEos2 | Other | Ampicillin | Vector Backbone:pDXA-HY; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:50 | 0 | |||
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B-HypaSpCas9 Resource Report Resource Website |
RRID:Addgene_126763 | B-HypaSpCas9 | S. pyogenes | Ampicillin | PMID:32144253 | pCbh-3xFLAG-NLS-Blackjack-HypaSpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations). Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-HypaSpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, HypaSpCas9. The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457). For detailed information and plasmid usage, please see the associated publication. | Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | N692A; M694A; Q695A; H698A; amino acids 1005-1013 replaced with two glycine | 2026-09-05 12:46:50 | 0 |
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B-HeFSpCas9 Resource Report Resource Website |
RRID:Addgene_126766 | B-HeFSpCas9 | S. pyogenes | Ampicillin | PMID:32144253 | pCbh-3xFLAG-NLS-Blackjack-HeFSpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations). Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-HeFSpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, HeFSpCas9. The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457). For detailed information and plasmid usage, please see the associated publication. | Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A; amino acids 1005-1013 replaced with two glycine | 2026-09-05 12:46:50 | 0 |
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B-evoSpCas9 Resource Report Resource Website |
RRID:Addgene_126765 | B-evoSpCas9 | S. pyogenes | Ampicillin | PMID:32144253 | pCbh-3xFLAG-NLS-Blackjack-evoSpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations). Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-evoSpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, evoSpCas9. The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457). For detailed information and plasmid usage, please see the associated publication. | Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | M495V; Y515N; K526E; R661Q; amino acids 1005-1013 replaced with two glycine | 2026-09-05 12:46:50 | 0 |
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SpCas9-HF1-plus Resource Report Resource Website |
RRID:Addgene_126768 | SpCas9-HF1-plus | S. pyogenes | Ampicillin | PMID:32144253 | pCbh-3xFLAG-NLS-SpCas9-HF1-plus-NLS (without U6-sgRNA coding sequence, with silent mutations). SpCas9-HF1-plus cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs. Shows almost the same fidelity and target selectivity as its parental increased fidelity variant, SpCas9-HF1. The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457). For detailed information and plasmid usage, please see the associated publication. | Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | N497A; Q695A; Q926A; amino acids 1005-1013 replaced with two glycine | 2026-09-05 12:46:50 | 0 |
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pCfB8748 Resource Report Resource Website |
RRID:Addgene_126918 | XdCrtE | Xanthophyllomyces dendrorhous | Ampicillin | PMID:31556952 | Vector Backbone:pCfB6677; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:51 | 0 | ||
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pCfB8382 Resource Report Resource Website |
RRID:Addgene_126916 | XdCrtI | Xanthophyllomyces dendrorhous | Ampicillin | PMID:31556952 | Vector Backbone:pCfB2904; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:51 | 0 | ||
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pET-21a(+) SurE D9A Resource Report Resource Website |
RRID:Addgene_12686 | SurE | Ampicillin | PMID:11524683 | Note: The SurE insert contains an additional mutation (Q12R) that the depositor has confirmed does not affect plasmid function. | Backbone Size:5443; Vector Backbone:pET-21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | changed Aspartate 9 to Alanine | 2026-09-05 12:46:51 | 0 | |
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pcDNA3.1 3xF-GAR1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_126873 | GAR1 | Homo sapiens | Ampicillin | PMID:20351177 | Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:51 | 1 | ||
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IDA3::NeonGreen exon2 (WS15) Resource Report Resource Website |
RRID:Addgene_126876 | IDA3 | Other | Ampicillin | PMID:29467251 | Vector Backbone:pHyg3; Vector Types:Other; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:51 | 0 | ||
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pCDNA5/to IRES-ECFP Resource Report Resource Website |
RRID:Addgene_126877 | Ampicillin | Vector Backbone:pCDNA5/to; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:51 | 0 | |||||
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IDA3::HA exon2 (WS13) Resource Report Resource Website |
RRID:Addgene_126874 | IDA3 | Other | Ampicillin | PMID:29467251 | Vector Backbone:pHyg3; Vector Types:Other; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:51 | 0 | ||
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IDA3::NeonGreen N-term (WS14) Resource Report Resource Website |
RRID:Addgene_126875 | IDA3 | Other | Ampicillin | PMID:29467251 | Vector Backbone:pHyg3; Vector Types:Other; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:51 | 0 | ||
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pCfB5191 Resource Report Resource Website |
RRID:Addgene_126911 | X-4 and XII-5 gRNA | Saccharomyces cerevisiae | Ampicillin | PMID:31556952 | Vector Backbone:pTAJAK-71; Vector Types:CRISPR; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:51 | 0 | ||
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pRSFDuet1-Cdc45 (delta 154–164) Resource Report Resource Website |
RRID:Addgene_126879 | Human Cdc45 | Homo sapiens | Kanamycin | PMID:27189187 | Backbone Size:3766; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | deletion amino acid 154-164 | 2026-09-05 12:46:51 | 0 | |
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pCfB8740 Resource Report Resource Website |
RRID:Addgene_126906 | XdCrtI | Xanthophyllomyces dendrorhous | Ampicillin | PMID:31556952 | Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:51 | 0 | ||
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pENTRY4_ Pi21 Resource Report Resource Website |
RRID:Addgene_126904 | Wheat_live_Cas9 | Synthetic | Kanamycin | Encodes gene specific sgRNAs driven by the wheat U6 promoter. | Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-09-05 12:46:51 | 0 | ||
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DCT.VV.Orf6 Resource Report Resource Website |
RRID:Addgene_12694 | VV.Orf6 | Vaccinia Virus | Ampicillin | PMID:15623576 | Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-05 12:46:52 | 0 |
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