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Species: Homo sapiens
Genetic Insert: Zer1
Vector Backbone Description: Vector Backbone:MSCV-IP N-V5; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22232672
Proper citation: RRID:Addgene_35100 Copy
Species: Homo sapiens
Genetic Insert: PSF
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pCMVmyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16024807
Proper citation: RRID:Addgene_35187 Copy
Species: Homo sapiens
Genetic Insert: PSF
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pCMVmyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16024807
Proper citation: RRID:Addgene_35185 Copy
Species: Homo sapiens
Genetic Insert: RPBI
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15037767
Comments: α-amanitin-resistant form of the largest subunit of pol II
Proper citation: RRID:Addgene_35179 Copy
Species: Homo sapiens
Genetic Insert: RPBI
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15037767
Comments: α-amanitin-resistant form of the largest subunit of pol II
Proper citation: RRID:Addgene_35177 Copy
Species: Homo sapiens
Genetic Insert: RPBI
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15037767
Comments: α-amanitin-resistant form of the largest subunit of pol II
Proper citation: RRID:Addgene_35176 Copy
Species: Homo sapiens
Genetic Insert: Protein Kinase C iota
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:11076; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the vector backbone, pWPI, is a bicistronic vector that allows for simultaneous expression of a transgene (PRKCI in this case) and an EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES.
The PRKCI insert in this plasmid is a known variant that lack the first nine amino acids of PRKCI when compared to GenBank entry NP_002731.4
Proper citation: RRID:Addgene_35387 Copy
Species: Homo sapiens
Genetic Insert: hPLK2 K111M
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11437; Vector Backbone:pWPI/Neomycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849493
Comments: The backbone of this bicistronic lentiviral vector was originally created by Dr. Didier Trono lab (pWPI, Plasmid # 12254), and then modified by Robert Strome using Neomycin to replace EGFP and adding several multiple clone sites. The MCS includes Swa I, Nde I, BamH I, Spe I, Xma I and Sma I (see sequence). The hPlk2WT cDNA was inserted with BamH I site, and can be replaced by any other gene with this site. This vector allows the simultaneous expression of hPlk2K111M and Neomycin to facilitate establishing stable mammalian transgene cell lines.
Please note that the full sequence for this plasmid is approximated and not fully verified. For further cloning strategies, please contact with Dr. Anurag Tandon.
Proper citation: RRID:Addgene_35386 Copy
Species: Homo sapiens
Genetic Insert: hPLK2
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11437; Vector Backbone:pWPI/Neomycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849493
Comments: The backbone of this bicistronic lentiviral vector was originally created by Dr. Didier Trono lab (pWPI, Plasmid # 12254), and then modified by Robert Strome using Neomycin to replace EGFP and adding several multiple clone sites. The MCS includes Swa I, Nde I, BamH I, Spe I, Xma I and Sma I (see sequence). The hPlk2WT cDNA was inserted with BamH I site, and can be replaced by any other gene with this site. This vector allows the simultaneous expression of hPlk2WT and Neomycin to facilitate establishing stable mammalian transgene cell lines.
Please note that the full sequence for this plasmid is approximated and not fully verified. For further cloning strategies, please contact with Dr. Anurag Tandon.
Proper citation: RRID:Addgene_35385 Copy
Species: Homo sapiens
Genetic Insert: PSF
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pCMVmyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16024807
Proper citation: RRID:Addgene_35377 Copy
Species: Homo sapiens
Genetic Insert: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2997; Vector Backbone:pGEM7Zf(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9214616
Proper citation: RRID:Addgene_35416 Copy
Species: Homo sapiens
Genetic Insert: DGK beta
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17272726
Proper citation: RRID:Addgene_35405 Copy
Species: Homo sapiens
Genetic Insert: DGK alpha
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17272726
Proper citation: RRID:Addgene_35404 Copy
Species: Homo sapiens
Genetic Insert: DGK iota
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17272726
Proper citation: RRID:Addgene_35409 Copy
Species: Homo sapiens
Genetic Insert: DGK gamma
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17272726
Proper citation: RRID:Addgene_35408 Copy
Species: Homo sapiens
Genetic Insert: DGK epsilon
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17272726
Proper citation: RRID:Addgene_35407 Copy
Species: Homo sapiens
Genetic Insert: 4xStat6 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4267; Vector Backbone:pGL4; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: This plasmid was generated by replacing the HIndIII(blunt)-AdeI fragment of p2xSTAT6-Luc2P (Addgene plasmid 35486) with the KpnI(blunt)-AdeI fragment of p2xSTAT6-Luc2P. The promoter insert consists of four tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG193.
Proper citation: RRID:Addgene_35554 Copy
Species: Homo sapiens
Genetic Insert: RARalpha
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15657432
Proper citation: RRID:Addgene_35557 Copy
Species: Homo sapiens
Genetic Insert: RARalpha
Vector Backbone Description: Vector Backbone:pcDNA 6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15657432
Proper citation: RRID:Addgene_35556 Copy
Species: Homo sapiens
Genetic Insert: promoter region of miR200b-200a-429 genomic cluster
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18829540
Comments: Please note that Addgene's sequencing results identified an insert at bp# 25/26 and a single nucleotide mismatch at bp# 38 when compared to the full plasmid sequence provided by the depositing laboratory. The insert at 25/26 and mismatch at 38 was due to an error made in assembling the promoter and plasmid sequences. Addgene's sequencing results are correct at these locations.
Proper citation: RRID:Addgene_35542 Copy
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