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Species: Homo sapiens
Genetic Insert: HLA-A*03:01
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEMEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00037 provides allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180451 Copy
Species: Mus musculus
Genetic Insert: Wnt-5b
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Proper citation: RRID:Addgene_18034 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*02:01 (CD8 mut)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Mutation prevents binding of CD8 (Purbhoo et al. (2001) J. Biol. Chem. doi.org/10.1074/jbc.M102498200). See https://www.ebi.ac.uk/ipd/imgt/hla/alleles/allele/?accession=HLA00005 for allele sequence data. For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180459 Copy
Species: Mus musculus
Genetic Insert: WNT-7A
Vector Backbone Description: Backbone Size:6620; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Gavin, B.J., McMahon, J.A., and McMahon, A.P. Expression of multiple novel Wnt-1/Int-1-related genes during fetal and adult mouse development. Genes Dev.,4:2319-2332, 1990.
Proper citation: RRID:Addgene_18036 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180334 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN9_v1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180339 Copy
Species: Homo sapiens
Genetic Insert: RPS6KA2
Vector Backbone Description: Backbone Size:8015; Vector Backbone:W118-1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180384 Copy
Species: Homo sapiens
Genetic Insert: RPS6KA2_K91A/K444A
Vector Backbone Description: Backbone Size:8015; Vector Backbone:W118-1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180385 Copy
Species: Homo sapiens
Genetic Insert: ZBTB7B
Vector Backbone Description: Backbone Size:8015; Vector Backbone:W118-1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180380 Copy
Genetic Insert: gRNAscr
Vector Backbone Description: Backbone Size:6487; Vector Backbone:pLenti_puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34588447
Proper citation: RRID:Addgene_180426 Copy
Species: Other
Genetic Insert: shControl
Vector Backbone Description: Backbone Size:6337; Vector Backbone:AAV.CBA.YFP.miR-E; Vector Types:AAV, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180386 Copy
Species: Mus musculus
Genetic Insert: shAtxn1
Vector Backbone Description: Backbone Size:6337; Vector Backbone:AAV.CBA.YFP.miR-E; Vector Types:AAV, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33709453
Proper citation: RRID:Addgene_180387 Copy
Genetic Insert: E2 Crimson
Vector Backbone Description: Backbone Marker:Jeffrey Barrick (Addgene plasmid # 110598); Vector Backbone:pBTK402; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35467987
Comments: Please note: Plasmid contains an 85bp discrepancy downstream of E2 Crimson at the BBa_B0015 Terminator compared to the depositor's provided sequence. This discrepancy is not known to affect plasmid function.
Proper citation: RRID:Addgene_180421 Copy
Vector Backbone Description: Backbone Size:8707; Vector Backbone:pXPR_502; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35113687
Proper citation: RRID:Addgene_180273 Copy
Genetic Insert: MCP-mCherry
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pCasper; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34003867
Proper citation: RRID:Addgene_180275 Copy
Species: Synthetic
Genetic Insert: PTPRC (CD45) sgRNA
Vector Backbone Description: Vector Backbone:CROPseq-Guide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35113687
Proper citation: RRID:Addgene_180271 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN7
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34854883
Comments: The mutant is the fusion of the SEPT9_i1-specific N-terminal sequence to SEPT9_i5.
Please note: Plasmid contains a 65bp insertion in the CloDF13 ori. This insertion is not known to affect plasmid function.
Proper citation: RRID:Addgene_180314 Copy
Species: Homo sapiens
Genetic Insert: human Otubain-1
Vector Backbone Description: Backbone Marker:TAKARA BIO; Backbone Size:5934; Vector Backbone:pRetroX-IRES-DsRedExpress Vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35159073
Comments: The backbone vector's cat.#632521 from TAKARA BIO
Proper citation: RRID:Addgene_180279 Copy
Species: Homo sapiens
Genetic Insert: SEPTIN2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34854883
Proper citation: RRID:Addgene_180319 Copy
Species: Synthetic
Genetic Insert: Multiple cloning site
Vector Backbone Description: Backbone Marker:Hyunju Ro; Backbone Size:7355; Vector Backbone:p13xQUAS-ZsGreen-P2A, α-cry:mCherry; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916605
Comments: Plasmid construct for binary IQ-Switch transgenesis
Proper citation: RRID:Addgene_180481 Copy
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