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Species: Vaccinia Virus
Genetic Insert: C ORF E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12692 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12691 Copy
Species: Synthetic
Genetic Insert: IV_CS10_1545_v1
Vector Backbone Description: Backbone Marker:Idem parts registry; Vector Backbone:pSB1A3; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30990672
Comments: Due to the large repeat sequences, plasmid cannot be verified by NGS or Sanger sequencing. Addgene recommends digesting the plasmid for verification.
Proper citation: RRID:Addgene_126861 Copy
Species: Synthetic
Genetic Insert: IV_CS8_7569_v1
Vector Backbone Description: Backbone Marker:Idem parts registry; Vector Backbone:pSB1A3; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30990672
Proper citation: RRID:Addgene_126865 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126902 Copy
Species: Synthetic
Genetic Insert: Wheat_live_Cas9
Vector Backbone Description: Vector Backbone:pENTR4; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Encodes gene specific sgRNAs driven by the wheat U6 promoter.
Proper citation: RRID:Addgene_126900 Copy
Species: Synthetic
Genetic Insert: II_CS3_7560_v1
Vector Backbone Description: Backbone Marker:Eurofins; Vector Backbone:pBSSK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30990672
Proper citation: RRID:Addgene_126853 Copy
Species: Synthetic
Genetic Insert: IV_CS6_4323_v1
Vector Backbone Description: Backbone Marker:Idem parts registry; Vector Backbone:pSB1A3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30990672
Proper citation: RRID:Addgene_126859 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP29-1A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126960 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP29-1B; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126961 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP49-2A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126964 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP18-Uac; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126959 Copy
Species: Homo sapiens
Genetic Insert: spacer against human genome with GC at cut site
Vector Backbone Description: Backbone Marker:Keith Joung; Backbone Size:2259; Vector Backbone:pSQT1313; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33753928
Comments: This construct was cloned by inverse PCR and in vivo recombination in E. coli strain SS320.
Proper citation: RRID:Addgene_126950 Copy
Species: Homo sapiens
Genetic Insert: spacer against human genome with CG at cut site
Vector Backbone Description: Backbone Marker:Keith Joung; Backbone Size:2259; Vector Backbone:pSQT1313; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33753928
Comments: This construct was cloned by inverse PCR and in vivo recombination in E. coli strain SS320.
Proper citation: RRID:Addgene_126953 Copy
Species: Homo sapiens
Genetic Insert: EGFP-SUMO interacting motif (SIM) from PIASx
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5000; Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27374333
Proper citation: RRID:Addgene_126954 Copy
Species: Homo sapiens
Genetic Insert: spacer against human genome with GA at cut site
Vector Backbone Description: Backbone Marker:Keith Joung; Backbone Size:2259; Vector Backbone:pSQT1313; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33753928
Comments: This construct was cloned by inverse PCR and in vivo recombination in E. coli strain SS320.
Proper citation: RRID:Addgene_126951 Copy
Vector Backbone Description: Backbone Marker:Backbone derived from SEVA.; Vector Backbone:pJUMP19-Pac; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33623824
Comments: Please visit https://www.biorxiv.org/content/10.1101/799585v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_126957 Copy
Species: Homo sapiens
Genetic Insert: EGFP-SUMO interacting motif (SIM) from PIASx
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5000; Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27374333
Proper citation: RRID:Addgene_126955 Copy
Species: Homo sapiens
Genetic Insert: 10 repeats of human SUMO3 each separated by (GGS)4
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5000; Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27374333
Proper citation: RRID:Addgene_126948 Copy
Species: Synthetic
Genetic Insert: SomArchon
Vector Backbone Description: Backbone Size:4813; Vector Backbone:pAAV-CaMKII; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597963
Proper citation: RRID:Addgene_126942 Copy
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