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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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EF.hICN1 Resource Report Resource Website |
RRID:Addgene_113715 | None | Ampicillin | PMID:31860685 | The hICN1 insert contains a difference that results in A184S compared to the NCBI reference. This difference does not impact the function of the plasmid. | Vector Backbone:EF.hICN1.CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:51 | 0 | ||
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pHOXB5.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113783 | HOXB5 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pHOXB5-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pCSDC2.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113781 | CSDC2 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pCSDC2-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pGLIS3.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113780 | GLIS3 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pGLIS3-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pTOX2.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113787 | TOX2 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTOX2-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pFOXF2.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113784 | FOXF2 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pFOXF2-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Resource Report Resource Website |
RRID:Addgene_113701 | SaCas9 gRNA Cassete | Synthetic | Ampicillin | PMID:30483052 | The H1TO promoter renders the gRNA inducible via the presence of Doxycycline. See: The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. 10.3389/fnmol.2016.00070 PubMed 27587996 | Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:51 | 0 | |
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pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette Resource Report Resource Website |
RRID:Addgene_113700 | SaCas9 gRNA Cassete | Synthetic | Ampicillin | PMID:30483052 | The H1TO promoter renders the gRNA inducible via the presence of Doxycycline. See: The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. 10.3389/fnmol.2016.00070 PubMed 27587996 | Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:51 | 0 | |
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pMuLE_ENTR_CBF-tdTomato_L3-L2 Resource Report Resource Website |
RRID:Addgene_113708 | CBF (notch dependent promoter) | Kanamycin | PMID:31860685 | Backbone Size:4403; Vector Backbone:pMuLE ENTR SV40 tdTomato L3-L2; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-09-01 09:12:51 | 0 | |||
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pATF4.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113772 | ATF4 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pATF4-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pZNF215.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113771 | ZNF215 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZNF215-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pE2F3.3.1-gDNA Resource Report Resource Website |
RRID:Addgene_113776 | E2F3 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. This is a nickase guide and must be used with the additional nickase plasmid pE2F3.3.2-gDNA and donor plasmid pE2F3-donor. | Backbone Marker:Zhang lab (Addgene plasmid ID: 62987); Vector Backbone:pX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pTCFL5.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113775 | TCFL5 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTCFL5-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pREST.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113774 | REST | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pREST-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pZFP3.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113779 | ZFP3 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZFP3-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR) Resource Report Resource Website |
RRID:Addgene_113762 | tdNfsB(F124W)-mCherry | E. coli | Ampicillin | PMID:30111097 | Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed." | Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin | F124W | 2026-09-01 09:12:52 | 0 |
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pZBTB12.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113769 | ZBTB12 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZBTB12-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pZNF561.1.0-gDNA Resource Report Resource Website |
RRID:Addgene_113768 | ZNF561 | Homo sapiens | Ampicillin | gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZNF561-donor | Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 0 | ||
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pVP91A-pcaHG Resource Report Resource Website 1+ mentions |
RRID:Addgene_113766 | β subunit of Protocatechuate 3,4- Dioxygenase | P. putida | Ampicillin | PMID:25548185 | Please ensure that the chosen expression cell line expresses the T5 RNA polymerase. Senavirathne, G., et al., Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule fluorescence imaging. Anal Biochem, 2018. 556: p. 78-84. | Backbone Marker:DNASU plasmid repository, Arizona State University Biodesign Institute; Backbone Size:4010; Vector Backbone:pVP91A vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:12:52 | 2 | |
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pENTR-R4R3-2xmEGFP-C Resource Report Resource Website 1+ mentions |
RRID:Addgene_113750 | mEGFP-mEGFP | Other | Kanamycin | PMID:31523744 | Second position vector for three-way Gateway LR reaction to insert C-terminal mEGFP-mEGFP at your locus of interest. | Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-09-01 09:12:51 | 1 |
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