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Species: Homo sapiens
Genetic Insert: U3-hTR-500
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pBS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12769858
Comments: Note that BLAST results bring back one T->C nt change in the TERC, but this is the plasmid used in the associated article.
Proper citation: RRID:Addgene_28170 Copy
Species: synthetic gene fragment
Genetic Insert: Tetrahymena TERT
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15731105
Proper citation: RRID:Addgene_28171 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pCAG-EGFP/RFP-int; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21209826
Proper citation: RRID:Addgene_28208 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pCAG-EGFP/RFP-int; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21209826
Proper citation: RRID:Addgene_28207 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pCAG-EGFP/RFP-int; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21209826
Proper citation: RRID:Addgene_28205 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Marker:Clontech Laboratories, Inc; Backbone Size:4694; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21209826
Proper citation: RRID:Addgene_28202 Copy
Species: Plasmodium falciparum
Genetic Insert: RAB GDP dissociation inhibitor
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7737; Vector Backbone:pET15-MHL (Addgene plasmid 26092); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 3P1W. http://www.thesgc.org/structures/3P1W/
Proper citation: RRID:Addgene_28167 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Marker:Clontech Laboratories, Inc; Backbone Size:4694; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21209826
Proper citation: RRID:Addgene_28200 Copy
Species: Homo sapiens
Genetic Insert: ARF1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3O47. http://www.thesgc.org/structures/3O47/
Proper citation: RRID:Addgene_28168 Copy
Species: Homo sapiens
Genetic Insert: Tar DNA binding protein
Vector Backbone Description: Backbone Marker:Clontech Laboratories, Inc; Backbone Size:4694; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21209826
Proper citation: RRID:Addgene_28201 Copy
Species: Homo sapiens
Genetic Insert: EPHB3
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:0; Vector Backbone:pFHMSP-LIC-N (Addgene plasmid 26095); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: PDB: 3P1I. http://www.thesgc.org/structures/3P1I/
Proper citation: RRID:Addgene_28165 Copy
Species: Homo sapiens
Genetic Insert: GTPase IMAP family member 2
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3P1J. http://www.thesgc.org/structures/3P1J/
Proper citation: RRID:Addgene_28166 Copy
Species: Homo sapiens
Genetic Insert: UHRF1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2L3R. http://www.thesgc.org/structures/2L3R/
Proper citation: RRID:Addgene_28163 Copy
Species: Mus musculus
Genetic Insert: Adcy4 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5000; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12239114
Comments: Contains a total of ~1812 bp of genomic DNA--i.e.,1459 bp upstream of the Adcy4 transcription start site and 353 bp downstream of the transcription start site. The downstream DNA includes exon I, intron I and the non-coding portion of exon II.
Proper citation: RRID:Addgene_28184 Copy
Species: Mus musculus
Genetic Insert: Oct4, Sox2, Klf4, c-Myc, Lin28
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:10200; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21243013
Comments: Please note that Addgene's sequencing results using the CMV-F and pCEP-F primers found 2 sequence variants within and after the CMV enhancer. One is a C->G mismatch at position 775 within the CMV enhancer and the second is a deletion at position 949 just after the end of the CAG enhancer, as compared to the full plasmid sequence provided by the depositing scientist. The significance of the two sequence variants is unclear; however, the plasmid functions as described in the associated publication. Addgene's other 4 sequencing results completely matched the depositor's sequence.
Proper citation: RRID:Addgene_28213 Copy
Species: Danio rerio
Genetic Insert: Unc5b mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20525357
Comments: See map and Genbank file for plasmid details.
Please note that Addgene's sequencing results identified 4 single nucleotide mismatches (at bp# 3373, 3376, 3380 and 3386) when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.
Proper citation: RRID:Addgene_28214 Copy
Genetic Insert: miRNAa
Vector Backbone Description: Backbone Size:6341; Vector Backbone:pCAG-RFP-KX; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21209826
Proper citation: RRID:Addgene_28211 Copy
Species: Mus musculus
Genetic Insert: Prkar1a
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pMT-REV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8388994
Comments: Expression vector encoding dominant-negative regulatory subunit of type 1 cAMP-dependent protein kinase.
pMT-REV is a zinc-inducible expression vector driven by the metallothionein promoter.
Proper citation: RRID:Addgene_28179 Copy
Genetic Insert: miRNAb
Vector Backbone Description: Backbone Size:6341; Vector Backbone:pCAG-RFP-KX; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21209826
Proper citation: RRID:Addgene_28212 Copy
Species: Homo sapiens
Genetic Insert: Nucleolin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16213212
Comments: There are a few mutations (K132R, K141E, & K142E and G680S) in the nucleolin but they are not thought to affect function.
Proper citation: RRID:Addgene_28176 Copy
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