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Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS.
Loop mutants have also all been cloned into this backbone for in vitro translation.
Proper citation: RRID:Addgene_26558 Copy
Genetic Insert: pT/HB
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2914; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: There are three mismatches between Addgene and author sequence but they are in vector backbone.
Proper citation: RRID:Addgene_26555 Copy
Species: Homo sapiens
Genetic Insert: TFIIB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11809839
Proper citation: RRID:Addgene_26675 Copy
Genetic Insert: pT2/HB
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2918; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_26557 Copy
Genetic Insert: pT2/BH
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2918; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_26556 Copy
Species: Homo sapiens
Genetic Insert: UBF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11285283
Proper citation: RRID:Addgene_26672 Copy
Vector Backbone Description: Backbone Size:10471; Vector Backbone:FSynIGW2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The vector has neuron specific promoter with downstream IRES2-EGFP sequence which allow the fluorescence marker protein to be expressed in a lower level under the same promoter with the transgene.
Proper citation: RRID:Addgene_26671 Copy
Species: Homo sapiens
Genetic Insert: Fibrillarin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11285283
Proper citation: RRID:Addgene_26673 Copy
Species: Bacteriophage P1
Genetic Insert: Cre
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCAG-IRES2-GFP; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17135424
Comments: Expresses Cre and GFP (driven by IRES)
Proper citation: RRID:Addgene_26646 Copy
Species: Epstein-Barr Virus
Genetic Insert: Epstein-Barr Nuclear Antigen 1
Vector Backbone Description: Backbone Size:5718; Vector Backbone:modified pHyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9830062
Comments: This plasmid expresses delta 205 GGA EBNA-1 driven by the CMV IE promoter.
Proper citation: RRID:Addgene_26648 Copy
Species: e. coli
Genetic Insert: pZE21-GFPaav
Vector Backbone Description: Backbone Size:2117; Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10659856
Comments: Note that there are some discrepancies between Addgene's QC sequence and the depositor's sequence. These differences are not known to affect function.
Proper citation: RRID:Addgene_26643 Copy
Species: Homo sapiens
Genetic Insert: Apoptotic protease activating factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16630894
Proper citation: RRID:Addgene_26642 Copy
Species: Mus musculus
Genetic Insert: Δ90beta catenin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Δ90AEGFP is Δ90betacatenin fused with EGFP-mut4 from Ami Okada
Proper citation: RRID:Addgene_26644 Copy
Genetic Insert: IHF
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Use Rosetta(DE3)plysS cells for expression. Please note that the IHF insert is NOT expressed with a His tag.
Proper citation: RRID:Addgene_26641 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2743; Vector Backbone:pGEM-3Z; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9514715
Comments: This plasmid contains a 147 bp nucleosome positioning sequence and is referred to as clone"603".
Please note that there may be discrepancies between Addgene's sequencing results and the depositor's sequence. Potential discrepancies are not a concern according to the depositing scientist because the clones were selected for their ability to bind to histone octamer, not for their specific sequence.
Proper citation: RRID:Addgene_26658 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2743; Vector Backbone:pGEM-3Z; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9514715
Comments: This plasmid contains a 147 bp nucleosome positioning sequence and is referred to as clone"602".
Please note that there may be discrepancies between Addgene's sequencing results and the depositor's sequence. Potential discrepancies are not a concern according to the depositing scientist because the clones were selected for their ability to bind to histone octamer, not for their specific sequence.
Proper citation: RRID:Addgene_26657 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2743; Vector Backbone:pGEM-3Z; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9514715
Comments: This plasmid contains a 147 bp nucleosome positioning sequence and is referred to as clone"605".
Please note that there may be discrepancies between Addgene's sequencing results and the depositor's sequence. Potential discrepancies are not a concern according to the depositing scientist because the clones were selected for their ability to bind to histone octamer, not for their specific sequence.
Proper citation: RRID:Addgene_26659 Copy
Species: Epstein-Barr Virus
Genetic Insert: Latent Membrane Protein 1
Vector Backbone Description: Backbone Size:4107; Vector Backbone:pSG5 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11387199
Proper citation: RRID:Addgene_26654 Copy
Species: Homo sapiens
Genetic Insert: β3-integrin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11967230
Proper citation: RRID:Addgene_26653 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2743; Vector Backbone:pGEM-3Z; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9514715
Comments: This plasmid contains a 147 bp nucleosome positioning sequence and is referred to as clone"601".
Primers that are used by the Widom lab to amplify the 147 bp fragment:
Forward:
ctggagaatcccggtgccg
Reverse:
acaggatgtatatatctgacacg
Please note that there may be discrepancies between Addgene's sequencing results and the depositor's sequence. Potential discrepancies are not a concern according to the depositing scientist because the clones were selected for their ability to bind to histone octamer, not for their specific sequence.
Proper citation: RRID:Addgene_26656 Copy
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