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Vector Backbone Description: Vector Backbone:pBAtC; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36456803
Proper citation: RRID:Addgene_189639 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35657952
Proper citation: RRID:Addgene_190245 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35657952
Proper citation: RRID:Addgene_190242 Copy
Genetic Insert: Mphot
Vector Backbone Description: Vector Backbone:pMpGE010; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29885839
Proper citation: RRID:Addgene_186729 Copy
Genetic Insert: sgRNA-Target1
Vector Backbone Description: Backbone Size:17689; Vector Backbone:pMpGE010; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35476214
Proper citation: RRID:Addgene_186295 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35657952
Proper citation: RRID:Addgene_190244 Copy
Species: E. coli
Genetic Insert: Retron Eco4 Reverse Transcriptase
Vector Backbone Description: Vector Backbone:pJKR-O-mphR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35896746
Comments: A192P mutation in mphR codon optimized does not affect plasmid function.
Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187201 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34124437
Comments: pSiMPls_N should be co-transformed with pSiMPls_C (or vice versa) in E. coli to get a reconstituted functional aminoglycoside adenylyltransferase thereby conferring resistance towards spectinomycin/streptomycin. The plasmids possess no additional antibiotic resistance gene.
Please see the Sequences link for the individual plasmid sequences.
An E. coli bacterial stab from Addgene will have both the plasmids pSiMPls_N + pSiMPls_C. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Proper citation: RRID:Addgene_167203 Copy
Species: Shewanella baltica OS195
Genetic Insert: Tetrathionate sensor kinase
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28373240
Proper citation: RRID:Addgene_90954 Copy
Species: Shewanella baltica OS195
Genetic Insert: Tetrathionate sensor kinase
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28373240
Proper citation: RRID:Addgene_90958 Copy
Species: Synthetic
Genetic Insert: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91209 Copy
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91206 Copy
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91205 Copy
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91203 Copy
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91200 Copy
Species: Other
Genetic Insert: gRNA targeting barley MLO
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91222 Copy
Species: Other
Genetic Insert: gRNA targeting tomato ANT1
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91223 Copy
Species: Other
Genetic Insert: gRNA targeting tomato ANT1
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91220 Copy
Species: Other
Genetic Insert: 2 gRNAs targeting tomato ARF8A
Vector Backbone Description: Vector Backbone:pDIRECT_10E; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91215 Copy
Species: Other
Genetic Insert: 2 gRNAs targeting tomato ARF8A
Vector Backbone Description: Vector Backbone:pDIRECT_10E; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91214 Copy
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