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Plasmids are provided by Addgene and DGRC.

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AatII-pRPS5A-PTP-3Xflag
 
Resource Report
Resource Website 		RRID:Addgene_189639 Spectinomycin PMID:36456803 Vector Backbone:pBAtC; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:14:22 0
pSelect-9
 
Resource Report
Resource Website 		RRID:Addgene_190245 LacI E. coli Spectinomycin PMID:35657952 Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:14:25 0
pSelect-2
 
Resource Report
Resource Website 		RRID:Addgene_190242 LacI E. coli Spectinomycin PMID:35657952 Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:14:25 0
pMpGE010-Target2 (Mpphot)
 
Resource Report
Resource Website 		RRID:Addgene_186729 Mphot Spectinomycin PMID:29885839 Vector Backbone:pMpGE010; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:14:34 0
pMpGE010-sgRNA-Target1 (MpRTN1)
 
Resource Report
Resource Website 		RRID:Addgene_186295 sgRNA-Target1 Spectinomycin PMID:35476214 Backbone Size:17689; Vector Backbone:pMpGE010; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:14:28 0
pSelect-7
 
Resource Report
Resource Website 		RRID:Addgene_190244 LacI E. coli Spectinomycin PMID:35657952 Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:14:30 0
pSLS.400
 
Resource Report
Resource Website 		RRID:Addgene_187201 Retron Eco4 Reverse Transcriptase E. coli Spectinomycin PMID:35896746 A192P mutation in mphR codon optimized does not affect plasmid function. Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint. Vector Backbone:pJKR-O-mphR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-29 01:14:30 0
pSiMPls_N + pSiMPls_C
 
Resource Report
Resource Website 		RRID:Addgene_167203 EGFP Other Spectinomycin PMID:34124437 pSiMPls_N should be co-transformed with pSiMPls_C (or vice versa) in E. coli to get a reconstituted functional aminoglycoside adenylyltransferase thereby conferring resistance towards spectinomycin/streptomycin. The plasmids possess no additional antibiotic resistance gene. Please see the Sequences link for the individual plasmid sequences. An E. coli bacterial stab from Addgene will have both the plasmids pSiMPls_N + pSiMPls_C. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:14:32 0
pKD227
 
Resource Report
Resource Website 		RRID:Addgene_90954 Tetrathionate sensor kinase Shewanella baltica OS195 Spectinomycin PMID:28373240 Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:51 0
pKD238-1a
 
Resource Report
Resource Website 		RRID:Addgene_90958 Tetrathionate sensor kinase Shewanella baltica OS195 Spectinomycin PMID:28373240 Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:51 0
pDIRECT_10E
 
Resource Report
Resource Website 		RRID:Addgene_91209 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers Synthetic Spectinomycin PMID:28522548 Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:53 0
pTRANS_160
 
Resource Report
Resource Website 		RRID:Addgene_91206 Spectinomycin PMID:28522548 Vector Backbone:pTRANS_100; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:53 0
pTRANS_150
 
Resource Report
Resource Website 		RRID:Addgene_91205 Spectinomycin PMID:28522548 Vector Backbone:pTRANS_100; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:53 0
pTRANS_120
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_91203 Spectinomycin PMID:28522548 Vector Backbone:pTRANS_100; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:53 1
pTRANS_102
 
Resource Report
Resource Website 		RRID:Addgene_91200 Spectinomycin PMID:28522548 Vector Backbone:pTRANS_100; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:53 0
pTC436
 
Resource Report
Resource Website 		RRID:Addgene_91222 gRNA targeting barley MLO Other Spectinomycin PMID:28522548 Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:54 0
pTC392
 
Resource Report
Resource Website 		RRID:Addgene_91223 gRNA targeting tomato ANT1 Other Spectinomycin PMID:28522548 Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:54 0
pTC391
 
Resource Report
Resource Website 		RRID:Addgene_91220 gRNA targeting tomato ANT1 Other Spectinomycin PMID:28522548 Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:54 0
pTC366
 
Resource Report
Resource Website 		RRID:Addgene_91215 2 gRNAs targeting tomato ARF8A Other Spectinomycin PMID:28522548 Vector Backbone:pDIRECT_10E; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:53 0
pTC365
 
Resource Report
Resource Website 		RRID:Addgene_91214 2 gRNAs targeting tomato ARF8A Other Spectinomycin PMID:28522548 Vector Backbone:pDIRECT_10E; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-29 01:12:53 0

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