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Species: Synthetic
Genetic Insert: Neuron-astrocyte proximity assay - Neuronal
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621490
Proper citation: RRID:Addgene_92283 Copy
Species: Synthetic
Genetic Insert: Neuron-astrocyte proximity assay - Astrocyte
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621490
Proper citation: RRID:Addgene_92281 Copy
Species: Gallus gallus
Genetic Insert: chick U6.3 promoter and gRNA cloning cassette
Vector Backbone Description: Backbone Size:3017; Vector Backbone:pNG3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29386245
Comments: Kudo, T., and S. Sutou. 2005. Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev. 51:411-417.
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687
Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.
Proper citation: RRID:Addgene_92359 Copy
Species: Other
Genetic Insert: Cas9 2A Citrine
Vector Backbone Description: Backbone Size:4944; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29386245
Comments: Betancur, P., M. Bronner-Fraser, and T. Sauka-Spengler. 2010. Genomic code for Sox10 activation reveals a key regulatory enhancer for cranial neural crest. Proc Natl Acad Sci U S A. 107:3570-3575.
Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.
Proper citation: RRID:Addgene_92358 Copy
Species: Other
Genetic Insert: mCherry and split EGFP
Vector Backbone Description: Vector Backbone:p23-phage; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27068474
Proper citation: RRID:Addgene_92351 Copy
Species: Homo sapiens
Genetic Insert: Nicn1 gene derived Alu element containing 3'UTR
Vector Backbone Description: Vector Backbone:pEGFP-c1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18497743
Proper citation: RRID:Addgene_92344 Copy
Vector Backbone Description: Vector Backbone:XPR_502; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30575746
Proper citation: RRID:Addgene_92379 Copy
Species: Homo sapiens
Genetic Insert: spastin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28495799
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.
Proper citation: RRID:Addgene_92368 Copy
Species: Homo sapiens
Genetic Insert: spastin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28495799
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.The endogenous M1 Kozak sequence was replaced with an optimal Kozak sequence to prevent leaky translation of M87 spastin
Proper citation: RRID:Addgene_92366 Copy
Species: Homo sapiens
Genetic Insert: spastin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28495799
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.The endogenous M1 Kozak sequence was replaced with an optimal Kozak sequence to prevent leaky translation of M87 spastin
Proper citation: RRID:Addgene_92365 Copy
Species: Gallus gallus
Genetic Insert: chick U6.1 promoter and gRNA cloning cassette
Vector Backbone Description: Backbone Size:3017; Vector Backbone:pNG1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29386245
Comments: Kudo, T., and S. Sutou. 2005. Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev. 51:411-417.
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687
Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.
Proper citation: RRID:Addgene_92395 Copy
Species: Synthetic
Genetic Insert: M13-TEV-C-P2A-TdTomato
Vector Backbone Description: Vector Backbone:pSyc 171 P2A vector; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28650460
Proper citation: RRID:Addgene_92391 Copy
Species: Synthetic
Genetic Insert: TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28650460
Proper citation: RRID:Addgene_92392 Copy
Species: Synthetic
Genetic Insert: TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Vector Backbone Description: Vector Backbone:pSyc 171 P2A vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28650460
Proper citation: RRID:Addgene_92390 Copy
Genetic Insert: 5HT6-YFP-Inpp5e
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:eYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28086093
Proper citation: RRID:Addgene_96808 Copy
Species: Mus musculus
Genetic Insert: VAMP8
Vector Backbone Description: Backbone Marker:CloneTech; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28524818
Comments: Please note that the VAMP8 sequence is from Uniprot (accession number O70404). Relative to the Genbank sequence (NM_016794.3), this construct carries mutation A36S.
Proper citation: RRID:Addgene_92424 Copy
Species: Homo sapiens
Genetic Insert: ANNEXINV
Vector Backbone Description: Backbone Marker:Tol2kit; Backbone Size:5800; Vector Backbone:pTol2pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26379496
Proper citation: RRID:Addgene_92388 Copy
Vector Backbone Description: Vector Backbone:XPR_050; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30575746
Proper citation: RRID:Addgene_92386 Copy
Vector Backbone Description: Vector Backbone:XPR_502; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30575746
Proper citation: RRID:Addgene_92384 Copy
Vector Backbone Description: Vector Backbone:XPR_501; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30575746
Proper citation: RRID:Addgene_92383 Copy
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