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Vector Backbone Description: Vector Backbone:XPR_502; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30575746
Proper citation: RRID:Addgene_92380 Copy
Species: Mus musculus
Genetic Insert: Ebf1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pTRE3G-IRES; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28137879
Proper citation: RRID:Addgene_96965 Copy
Species: Arabidopsis thaliana
Genetic Insert: SEIPIN1
Vector Backbone Description: Backbone Size:12460; Vector Backbone:pMDC84; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26362606
Comments: The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html
Proper citation: RRID:Addgene_96979 Copy
Genetic Insert: FLIM Sensor for PKA Activity in Cytosol
Vector Backbone Description: Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28285819
Comments: Harvey, C.D., Ehrhardt, A.G., Cellurale, C., Zhong, H., Yasuda, R., Davis, R.J., and Svoboda, K. (2008). A genetically encoded fluorescent sensor of ERK activity. Proc. Natl. Acad. Sci. USA 105, 19264–19269.
Komatsu, N., Aoki, K., Yamada, M., Yukinaga, H., Fujita, Y., Kamioka, Y., and Matsuda, M. (2011). Development of an optimized backbone of FRET biosensors for kinases and GTPases. Mol. Biol. Cell 22, 4647–4656.
Proper citation: RRID:Addgene_96897 Copy
Species: Synthetic
Genetic Insert: Monomeric Cerulean 3
Vector Backbone Description: Backbone Size:6295; Vector Backbone:MSCVpuro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29533508
Comments: Base vector obtained from Robert Hawley.
Proper citation: RRID:Addgene_96936 Copy
Species: Homo sapiens
Genetic Insert: EGFP and human DMPK exons 11-15 with 0 CTG repeats
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pBiTet; Vector Types:Mammalian Expression, tetracycline responsive and bidirectional; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371589
Proper citation: RRID:Addgene_96903 Copy
Species: Homo sapiens
Genetic Insert: EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pBiTet; Vector Types:Mammalian Expression, tetracycline responsive and bidirectional; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371589
Proper citation: RRID:Addgene_96905 Copy
Species: Other
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pXPR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29251726
Proper citation: RRID:Addgene_96917 Copy
Species: Homo sapiens
Genetic Insert: sgRNA NEAT1pr_v2
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #42230); Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28325845
Proper citation: RRID:Addgene_97082 Copy
Species: Homo sapiens
Genetic Insert: NEAT1m_v1-RT
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28325845
Comments: The left arm of the repair template was cloned using the AseI site. The right arm was cloned into the MluI site. Original EGFP was mutated to EYFP using mutation: L65F, T66G, V69L, S73A, T204Y
Proper citation: RRID:Addgene_97089 Copy
Species: Mus musculus
Genetic Insert: Rosa26 sgRNA
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:8489; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35086530
Comments: This plasmid contains a Cas9 gene and a Rosa26-specific sgRNA for CRISPR/Cas9 mediated targeting of large cassettes into the mouse Rosa26 locus.
Proper citation: RRID:Addgene_97007 Copy
Vector Backbone Description: Backbone Size:4383; Vector Backbone:p15a ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35086530
Comments: This plasmid is for ES cell targeting into the Rosa26 locus. It consists of a 5' homology arm and a 3' homology arm only. A cassette of interest can be cloned into a PacI site between the two arms. This vector lacks the sgRNA target sequence found in the px330_Rosa_sgRNA plasmid.
Proper citation: RRID:Addgene_97008 Copy
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28954734
Proper citation: RRID:Addgene_97101 Copy
Species: Homo sapiens
Genetic Insert: Spi-1 Proto-Oncogene
Vector Backbone Description: Backbone Marker:Stephen Elledge, Thomas Westbrook ; Backbone Size:12152; Vector Backbone:pINDUCER-21; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28514439
Proper citation: RRID:Addgene_97039 Copy
Species: Synthetic
Genetic Insert: Actb HMEJ donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28524166
Comments: INSERT2:EGFP driven by EF1a promoter; INSERT3:U6-driven sgRNAs targeting Actb
Proper citation: RRID:Addgene_97308 Copy
Genetic Insert: Pin domain
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27118836
Comments: [GG] is a Golden Gate cloning site, a BsaI enzyme restriction site with sticky ends CGAG___ACGC, as described in the cloning scheme of Figure S1. The Pin domain silencing experiment is shown in Figure S4.
Proper citation: RRID:Addgene_97191 Copy
Species: Homo sapiens
Genetic Insert: Nuak1
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6299; Vector Backbone:3xflag-CMV plasmid; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27720485
Proper citation: RRID:Addgene_97219 Copy
Vector Backbone Description: Backbone Size:10044; Vector Backbone:pFUSE-DEST_R1R4; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28512244
Comments: Recombine Gene A + Gene B into this Destination vector by Gateway LR reaction to make the final fusion.
The pFUSE-A_P1P2 is the same plasmid as Gateway™ pDONR™221 Vector and is commercially available from ThermoFisher Scientific (Cat#: 12536017).
Proper citation: RRID:Addgene_97186 Copy
Species: Danio rerio
Genetic Insert: AcGFP
Vector Backbone Description: Vector Backbone:Tol2 backbone; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29444433
Proper citation: RRID:Addgene_97151 Copy
Species: Synthetic
Genetic Insert: Actb NHEJ donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28524166
Comments: INSERT2:EGFP driven by EF1a promoter; INSERT3:U6-driven sgRNAs targeting Actb
Proper citation: RRID:Addgene_97310 Copy
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