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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B
 
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RRID:Addgene_92380 Ampicillin PMID:30575746 Vector Backbone:XPR_502; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 10:05:28 2
pTRE3G-EBF1
 
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RRID:Addgene_96965 Ebf1 Mus musculus Ampicillin PMID:28137879 Backbone Marker:Clontech; Vector Backbone:pTRE3G-IRES; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 10:05:30 1
pMDC84-SEIPIN1
 
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RRID:Addgene_96979 SEIPIN1 Arabidopsis thaliana Kanamycin PMID:26362606 The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html Backbone Size:12460; Vector Backbone:pMDC84; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin 2026-09-01 10:05:30 1
AKARet-cyto
 
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RRID:Addgene_96897 FLIM Sensor for PKA Activity in Cytosol Ampicillin PMID:28285819 Harvey, C.D., Ehrhardt, A.G., Cellurale, C., Zhong, H., Yasuda, R., Davis, R.J., and Svoboda, K. (2008). A genetically encoded fluorescent sensor of ERK activity. Proc. Natl. Acad. Sci. USA 105, 19264–19269. Komatsu, N., Aoki, K., Yamada, M., Yukinaga, H., Fujita, Y., Kamioka, Y., and Matsuda, M. (2011). Development of an optimized backbone of FRET biosensors for kinases and GTPases. Mol. Biol. Cell 22, 4647–4656. Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 10:05:29 1
pMSCVpuro-mCerulean3
 
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RRID:Addgene_96936 Monomeric Cerulean 3 Synthetic Ampicillin PMID:29533508 Base vector obtained from Robert Hawley. Backbone Size:6295; Vector Backbone:MSCVpuro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 10:05:30 1
pBItetDMPKSGFP
 
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RRID:Addgene_96903 EGFP and human DMPK exons 11-15 with 0 CTG repeats Homo sapiens Ampicillin PMID:22371589 Backbone Size:2800; Vector Backbone:pBiTet; Vector Types:Mammalian Expression, tetracycline responsive and bidirectional; Bacterial Resistance:Ampicillin 2026-09-01 10:05:29 3
pBItetDT480GFP
 
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RRID:Addgene_96905 EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats Homo sapiens Ampicillin PMID:22371589 Backbone Size:2800; Vector Backbone:pBiTet; Vector Types:Mammalian Expression, tetracycline responsive and bidirectional; Bacterial Resistance:Ampicillin 2026-09-01 10:05:29 1
pXPR_120
 
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RRID:Addgene_96917 dCas9 Other Ampicillin PMID:29251726 Vector Backbone:pXPR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin D10A, N863A 2026-09-01 10:05:30 7
pX330-NEAT1pr_v2
 
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RRID:Addgene_97082 sgRNA NEAT1pr_v2 Homo sapiens Ampicillin PMID:28325845 Backbone Marker:Feng Zhang (Addgene #42230); Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 10:05:32 1
pYFP-NEAT1m_v1-RT
 
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RRID:Addgene_97089 NEAT1m_v1-RT Homo sapiens Kanamycin PMID:28325845 The left arm of the repair template was cloned using the AseI site. The right arm was cloned into the MluI site. Original EGFP was mutated to EYFP using mutation: L65F, T66G, V69L, S73A, T204Y Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:CRISPR; Bacterial Resistance:Kanamycin 2026-09-01 10:05:32 1
px330_Rosa_sgRNA
 
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RRID:Addgene_97007 Rosa26 sgRNA Mus musculus Ampicillin PMID:35086530 This plasmid contains a Cas9 gene and a Rosa26-specific sgRNA for CRISPR/Cas9 mediated targeting of large cassettes into the mouse Rosa26 locus. Backbone Marker:Feng Zhang; Backbone Size:8489; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-09-01 10:05:31 1
p15a_Kan_MLS_Rosa26_Short_CRISPRDel
 
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RRID:Addgene_97008 Kanamycin PMID:35086530 This plasmid is for ES cell targeting into the Rosa26 locus. It consists of a 5' homology arm and a 3' homology arm only. A cassette of interest can be cloned into a PacI site between the two arms. This vector lacks the sgRNA target sequence found in the px330_Rosa_sgRNA plasmid. Backbone Size:4383; Vector Backbone:p15a ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-09-01 10:05:31 1
Tol2-lyzC-Vector-Dendra2
 
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RRID:Addgene_97101 Ampicillin PMID:28954734 Vector Backbone:Tol2; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin 2026-09-01 10:05:32 1
pINDUCER-21-SPI1
 
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RRID:Addgene_97039 Spi-1 Proto-Oncogene Homo sapiens Ampicillin PMID:28514439 Backbone Marker:Stephen Elledge, Thomas Westbrook ; Backbone Size:12152; Vector Backbone:pINDUCER-21; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-09-01 10:05:31 4
AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
 
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RRID:Addgene_97308 Actb HMEJ donor Synthetic Ampicillin PMID:28524166 INSERT2:EGFP driven by EF1a promoter; INSERT3:U6-driven sgRNAs targeting Actb Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin 2026-09-01 10:05:33 2
[GG][Pin]
 
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RRID:Addgene_97191 Pin domain Ampicillin PMID:27118836 [GG] is a Golden Gate cloning site, a BsaI enzyme restriction site with sticky ends CGAG___ACGC, as described in the cloning scheme of Figure S1. The Pin domain silencing experiment is shown in Figure S4. Backbone Marker:Promega; Vector Backbone:pCI; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-09-01 10:05:33 1
K84M kinase-dead Nuak1-3xflag
 
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RRID:Addgene_97219 Nuak1 Homo sapiens Ampicillin PMID:27720485 Backbone Marker:Sigma-Aldrich; Backbone Size:6299; Vector Backbone:3xflag-CMV plasmid; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin Kinase-dead K84M 2026-09-01 10:05:33 1
pFUSE-DEST_R1R4
 
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RRID:Addgene_97186 Chloramphenicol and Ampicillin PMID:28512244 Recombine Gene A + Gene B into this Destination vector by Gateway LR reaction to make the final fusion. The pFUSE-A_P1P2 is the same plasmid as Gateway™ pDONR™221 Vector and is commercially available from ThermoFisher Scientific (Cat#: 12536017). Backbone Size:10044; Vector Backbone:pFUSE-DEST_R1R4; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-09-01 10:05:33 1
Tol2-krt4-GFP
 
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RRID:Addgene_97151 AcGFP Danio rerio Ampicillin PMID:29444433 Vector Backbone:Tol2 backbone; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin 2026-09-01 10:05:33 1
AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA
 
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RRID:Addgene_97310 Actb NHEJ donor Synthetic Ampicillin PMID:28524166 INSERT2:EGFP driven by EF1a promoter; INSERT3:U6-driven sgRNAs targeting Actb Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin 2026-09-01 10:05:33 1

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