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Species: Homo sapiens
Genetic Insert: Karyopherin Alpha 3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20701745
Proper citation: RRID:Addgene_26679 Copy
Species: Homo sapiens
Genetic Insert: Rs1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4879; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21375737
Comments: pcDNA3.2 expression vector with the majority of the CMV promoter deleted, and carrying the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences
Proper citation: RRID:Addgene_26797 Copy
Species: C. familiaris (dog RNAi)
Genetic Insert: dog Rab25 RNAi
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20890297
Comments: Matches dog and Panda Rab25. RNAi sequence is: 5'-AGAGATCTTCACCAAAGTG-3'
Proper citation: RRID:Addgene_26711 Copy
Genetic Insert: multiple cloning sequence
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21375737
Comments: Empty entry vector carrying Gateway R3L2 sites, with MCS. This plasmid can act as an empty carrier when combined with a L1L3 plasmid to generate expression plasmids carrying just the L1L3 component.
This plasmid was constructed as follows: The pEntr2B entry vector was digested with AflII and XhoI to remove the attL1 and ccdb genes. Oligos
containing the attR3 site flanked by AflII on the 5' end and a polylinker (NotI-PacI-Bsu36I-NdeI-XhoI) on the 3' end were ligated to generate pEntR3L2-MCS.
Proper citation: RRID:Addgene_26799 Copy
Species: Homo sapiens
Genetic Insert: Karyopherin Alpha 1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20701745
Comments: S73N is a reported SNP in the KPNA1 sequence. For more information, please see: https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?type=rs&rs=4678193
Proper citation: RRID:Addgene_26677 Copy
Genetic Insert: multiple cloning sequence
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21375737
Comments: Empty entry vector carrying Gateway L1L3 sites, with MCS. This plasmid can act as an empty carrier when combined with a R3L2 plasmid to generate expression plasmids carrying just the R3L2 component (i.e., TetO-transgene expressing plasmid)
Plasmid was constructed as follows: The pEntr2B entry vector (Invitrogen) was digested with PstI and XhoI to remove the attL2 site.
Oligonucleotides containing SalI-XhoI-Bsu36I-NdeI-PacI-EcoRI-NotI flanking the attL3 sequence on the 5' side
and PstI on the 3' side were ligated in to create an intermediate plasmid carrying attL1 and attL3 sites. A LoxP sequence was introduced at the XhoI site.
Proper citation: RRID:Addgene_26798 Copy
Genetic Insert: GFP-ZFN1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: The original paper describing this plasmid should also be cited: Pruett-Miller, S.M., Connelly, J.P., Maeder, M.L., Joung, J.K., and Porteus, M.H. (2008). Comparison of zinc finger nucleases for use in gene targeting in mammalian cells. Mol. Ther. 16, 707–717.
Proper citation: RRID:Addgene_26791 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Backbone Marker:Bob Weinberg, Addgene plasmid 1764; Backbone Size:5200; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20551166
Comments: H2B-GFP was cloned from pBOS-H2B-GFP (BD Biosciences) into the pBABE retroviral vector.
Proper citation: RRID:Addgene_26790 Copy
Species: Homo sapiens
Genetic Insert: Guanidinoacetate N-methyltransferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5404; Vector Backbone:pGL4.20; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19917247
Comments: There are a few discrepancies between Addgene quality control sequence and NCBI reference sequences, however, these do not affect function of the plasmid.
Proper citation: RRID:Addgene_26804 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20729842
Proper citation: RRID:Addgene_26768 Copy
Species: Homo sapiens
Genetic Insert: eNpHR 3.0
Vector Backbone Description: Backbone Size:8433; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20303157
Comments: This plasmid contains the human synapsin I promoter.
For additional information please visit - http://www.optogenetics.org
Proper citation: RRID:Addgene_26775 Copy
Species: Homo sapiens
Genetic Insert: S100B
Vector Backbone Description: Backbone Marker:Stan Dunn; Backbone Size:5696; Vector Backbone:pSD80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8688416
Comments: There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid.
Proper citation: RRID:Addgene_26774 Copy
Genetic Insert: EF1a-GFP-IRES-Puro
Vector Backbone Description: Backbone Size:7063; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: Allows for puromycin selection of stable integration of GFP expression cassette. With modification, this vector enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting.
Proper citation: RRID:Addgene_26777 Copy
Genetic Insert: GFP*35
Vector Backbone Description: Backbone Size:7063; Vector Backbone:pEGIP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Proper citation: RRID:Addgene_26776 Copy
Species: Aequorea victoria and Discosoma sp
Genetic Insert: CAG-TdTomato-2A-H2BGFP-SV40pA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18793381
Proper citation: RRID:Addgene_26771 Copy
Species: Anabaena
Genetic Insert: iLOV
Vector Backbone Description: Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060199
Proper citation: RRID:Addgene_26770 Copy
Species: Aequorea victoria, Discosoma sp
Genetic Insert: TdTomato-2A-H2BGFP-SV40pA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18793381
Proper citation: RRID:Addgene_26773 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, FLP/FRT; PhiC31/attB-attP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20953177
Comments: The pDRAV plasmid series encode a 5' loxP site and a 3' FRT site in all possible orientations for dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521
Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocols/1906
Proper citation: RRID:Addgene_26749 Copy
Species: Homo sapiens
Genetic Insert: ensconsin
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, probe for microtubules; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060892
Comments: The microtubule binding domain of ensconsin (EMTB) was fused to 3 GFP molecules in order to visualize microtubules. For cloning details see Faire et al., 1999 Journal
of Cell Science 112, 4243-4255.
Note: It is difficult to sequence entirely through 3 tandem GFPs. Users are encouraged to perform a double-digest on this plasmid with EcoRI and XhoI to verify the presence of a ~3kb band corresponding to the EMTB insert + 3GFPs. Please see above attachment.
Proper citation: RRID:Addgene_26741 Copy
Genetic Insert: CFP, mCherry, and cI-YFP
Vector Backbone Description: Backbone Marker:Elowitz Lab; Backbone Size:3700; Vector Backbone:pZS21-cIYFP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19029898
Proper citation: RRID:Addgene_26756 Copy
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