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Species: Synthetic
Genetic Insert: Actb HR donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28524166
Proper citation: RRID:Addgene_97317 Copy
Species: Homo sapiens
Genetic Insert: PTEN 3' UTR
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4500; Vector Backbone:pCMV-MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32314732
Comments: pCMV-PTEN-3'UTR was created by sub-cloning partial fragments of PTEN 3'UTR from HeLa gDNA using Gibson Assembly. The cloned insert was confirmed by DNA sequencing.
Proper citation: RRID:Addgene_97204 Copy
Species: Mus musculus
Genetic Insert: Ascl1
Vector Backbone Description: Backbone Size:7776; Vector Backbone:Teto-FUW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28504679
Proper citation: RRID:Addgene_97329 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExogron Rab11b knock in. DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation. Lentiviral delivery of AtAFBP2 and TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179904 Copy
Species: Homo sapiens
Genetic Insert: RAB11A, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab11a knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
AntiGFPnanobody can be used to manipulate with endogenously expressed protein.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179902 Copy
Species: Methanosarcina mazei
Genetic Insert: codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182287 Copy
Species: Homo sapiens
Genetic Insert: RAB25, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab25 knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179900 Copy
Species: Homo sapiens
Genetic Insert: hM3D (Gq)
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:2900; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35443152
Proper citation: RRID:Addgene_184753 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtAFB2
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6390; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: This lentiviral plasmids delivers AtAFB2 (auxin-inducible degron system) fused to mTagBFP2. In combination with TetOn3G can be used to generate DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179889 Copy
Species: Synthetic
Genetic Insert: TetOn3G
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6370; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Lentiviral vector encoding Tet-On 3G constitutively expressed by EF1a promoter. Inducible promoter TRE3GS has to be delivered separately (e.g. DExCon knock in = Doxycycline-mediated endogenous gene Expression Control)
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179887 Copy
Genetic Insert: TetOn3G
Vector Backbone Description: Backbone Marker:Systembio; Backbone Size:6370; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Lentiviral vector encoding Tet-On 3G constitutively expressed by EF1a promoter. Inducible promoter TRE3GS has to be delivered separately (e.g. DExCon knock in = Doxycycline-mediated endogenous gene Expression Control)
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179888 Copy
Species: Synthetic
Genetic Insert: iRFP702-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184455 Copy
Species: Synthetic
Genetic Insert: mCardinal-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184453 Copy
Species: Synthetic
Genetic Insert: LiLac fluorescent sensor of lactate
Vector Backbone Description: Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35614105
Comments: mTurquoise2 sequences are originally from Goedhart, J. et al. Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat Commun 3, 751 (2012) and were obtained by PCR from the plasmid encoding the Lck-cpmTq2-Calcium-lifetime-sensor (Addgene plasmid #129627) (A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium. van der Linden FH, Mahlandt EK, Arts JJG, Beumer J, Puschhof J, de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ Jr, Goedhart J. Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w.)
Proper citation: RRID:Addgene_184570 Copy
Species: Synthetic
Genetic Insert: iRFP702-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184457 Copy
Species: Homo sapiens
Genetic Insert: RAB25, member RAS oncogene family
Vector Backbone Description: Backbone Marker:GeneArt/Life Technologies; Backbone Size:3000; Vector Backbone:pMK-RQ; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab25 knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179896 Copy
Species: Homo sapiens
Genetic Insert: RAB11A, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab11a knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179897 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for integration of SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out) to Rab11b locus
This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11b expression (from the second allele) on demand
DExCon = Doxycycline-mediated endogenous gene Expression Control
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179895 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon mCherry Rab11b knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179898 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon mNeonGreen Rab11b knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179899 Copy
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