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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCMVTNT-T7-KPNA3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26679 | Karyopherin Alpha 3 | Homo sapiens | Ampicillin | PMID:20701745 | Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:09 | 5 | ||
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Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1) Resource Report Resource Website |
RRID:Addgene_26797 | Rs1 | Homo sapiens | Ampicillin | PMID:21375737 | pcDNA3.2 expression vector with the majority of the CMV promoter deleted, and carrying the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences | Backbone Marker:Invitrogen; Backbone Size:4879; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D100A | 2026-08-29 01:03:11 | 0 |
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pLKO.1-blast-cfRab25_2 Resource Report Resource Website |
RRID:Addgene_26711 | dog Rab25 RNAi | C. familiaris (dog RNAi) | Ampicillin | PMID:20890297 | Matches dog and Panda Rab25. RNAi sequence is: 5'-AGAGATCTTCACCAAAGTG-3' | Backbone Size:7000; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin | RNAi to canine Rab25. | 2026-08-29 01:03:10 | 0 |
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pEnt R3L2-MCS Resource Report Resource Website |
RRID:Addgene_26799 | multiple cloning sequence | Kanamycin | PMID:21375737 | Empty entry vector carrying Gateway R3L2 sites, with MCS. This plasmid can act as an empty carrier when combined with a L1L3 plasmid to generate expression plasmids carrying just the L1L3 component. This plasmid was constructed as follows: The pEntr2B entry vector was digested with AflII and XhoI to remove the attL1 and ccdb genes. Oligos containing the attR3 site flanked by AflII on the 5' end and a polylinker (NotI-PacI-Bsu36I-NdeI-XhoI) on the 3' end were ligated to generate pEntR3L2-MCS. | Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-29 01:03:11 | 0 | ||
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pCMVTNT-T7-KPNA1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26677 | Karyopherin Alpha 1 | Homo sapiens | Ampicillin | PMID:20701745 | S73N is a reported SNP in the KPNA1 sequence. For more information, please see: https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?type=rs&rs=4678193 | Backbone Marker:Promega; Backbone Size:4050; Vector Backbone:pCMVTNT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | KPNA1 sequence contains a reported SNP at S73N | 2026-08-29 01:03:09 | 7 |
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pEnt L1L3-MCS Resource Report Resource Website |
RRID:Addgene_26798 | multiple cloning sequence | Kanamycin | PMID:21375737 | Empty entry vector carrying Gateway L1L3 sites, with MCS. This plasmid can act as an empty carrier when combined with a R3L2 plasmid to generate expression plasmids carrying just the R3L2 component (i.e., TetO-transgene expressing plasmid) Plasmid was constructed as follows: The pEntr2B entry vector (Invitrogen) was digested with PstI and XhoI to remove the attL2 site. Oligonucleotides containing SalI-XhoI-Bsu36I-NdeI-PacI-EcoRI-NotI flanking the attL3 sequence on the 5' side and PstI on the 3' side were ligated in to create an intermediate plasmid carrying attL1 and attL3 sites. A LoxP sequence was introduced at the XhoI site. | Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-29 01:03:11 | 0 | ||
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GFPZFN1.4-B2H Resource Report Resource Website 1+ mentions |
RRID:Addgene_26791 | GFP-ZFN1 | Ampicillin | PMID:19540188 | The original paper describing this plasmid should also be cited: Pruett-Miller, S.M., Connelly, J.P., Maeder, M.L., Joung, J.K., and Porteus, M.H. (2008). Comparison of zinc finger nucleases for use in gene targeting in mammalian cells. Mol. Ther. 16, 707–717. | Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:11 | 2 | ||
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pBABE-H2BGFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_26790 | H2B-GFP | Homo sapiens | Ampicillin | PMID:20551166 | H2B-GFP was cloned from pBOS-H2B-GFP (BD Biosciences) into the pBABE retroviral vector. | Backbone Marker:Bob Weinberg, Addgene plasmid 1764; Backbone Size:5200; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:11 | 12 | |
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-4200GAMT-P1-pGL4 Resource Report Resource Website |
RRID:Addgene_26804 | Guanidinoacetate N-methyltransferase | Homo sapiens | Ampicillin | PMID:19917247 | There are a few discrepancies between Addgene quality control sequence and NCBI reference sequences, however, these do not affect function of the plasmid. | Backbone Marker:Promega; Backbone Size:5404; Vector Backbone:pGL4.20; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:11 | 0 | |
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pShuttle mCherry-Tubulin Resource Report Resource Website 1+ mentions |
RRID:Addgene_26768 | Tubulin | Homo sapiens | Kanamycin | PMID:20729842 | Backbone Marker:Agilent Technologies; Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin | 2026-08-29 01:03:10 | 5 | ||
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pLenti-hSyn-eNpHR 3.0-EYFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_26775 | eNpHR 3.0 | Homo sapiens | Ampicillin | PMID:20303157 | This plasmid contains the human synapsin I promoter. For additional information please visit - http://www.optogenetics.org | Backbone Size:8433; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Trafficking Signal (TS) ER Export Signal | 2026-08-29 01:03:11 | 7 |
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pSS2 (hS100B) Resource Report Resource Website 1+ mentions |
RRID:Addgene_26774 | S100B | Homo sapiens | Ampicillin | PMID:8688416 | There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid. | Backbone Marker:Stan Dunn; Backbone Size:5696; Vector Backbone:pSD80; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:11 | 2 | |
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pEGIP Resource Report Resource Website 10+ mentions |
RRID:Addgene_26777 | EF1a-GFP-IRES-Puro | Ampicillin | PMID:19540188 | Allows for puromycin selection of stable integration of GFP expression cassette. With modification, this vector enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting. | Backbone Size:7063; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:11 | 38 | ||
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pEGIP*35 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26776 | GFP*35 | Ampicillin | PMID:19540188 | Backbone Size:7063; Vector Backbone:pEGIP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:11 | 3 | |||
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pCAG-TAG Resource Report Resource Website 10+ mentions |
RRID:Addgene_26771 | CAG-TdTomato-2A-H2BGFP-SV40pA | Aequorea victoria and Discosoma sp | Ampicillin | PMID:18793381 | Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:11 | 11 | ||
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pUC57 iLOV ANAB Resource Report Resource Website |
RRID:Addgene_26770 | iLOV | Anabaena | Ampicillin | PMID:19060199 | Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:11 | 0 | ||
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pCS2-Tm2AG Resource Report Resource Website |
RRID:Addgene_26773 | TdTomato-2A-H2BGFP-SV40pA | Aequorea victoria, Discosoma sp | Ampicillin | PMID:18793381 | Backbone Size:4000; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | mutation in 2A peptide that renders it non-functional (so the TdTomato and H2BGFP do not form two separate proteins, but remain fused) | 2026-08-29 01:03:11 | 0 | |
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pDRAV-4 Resource Report Resource Website |
RRID:Addgene_26749 | Ampicillin | PMID:20953177 | The pDRAV plasmid series encode a 5' loxP site and a 3' FRT site in all possible orientations for dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521 Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocols/1906 | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, FLP/FRT; PhiC31/attB-attP; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:10 | 0 | |||
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EMTB-3XGFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_26741 | ensconsin | Homo sapiens | Ampicillin | PMID:19060892 | The microtubule binding domain of ensconsin (EMTB) was fused to 3 GFP molecules in order to visualize microtubules. For cloning details see Faire et al., 1999 Journal of Cell Science 112, 4243-4255. Note: It is difficult to sequence entirely through 3 tandem GFPs. Users are encouraged to perform a double-digest on this plasmid with EcoRI and XhoI to verify the presence of a ~3kb band corresponding to the EMTB insert + 3GFPs. Please see above attachment. | Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, probe for microtubules; Bacterial Resistance:Ampicillin | Only contains the microtubule binding-domain of ensconsin (amino acids 18-283 of GenBank reference sequence X73882) | 2026-08-29 01:03:10 | 33 |
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pNS2-sigmaVL Resource Report Resource Website 1+ mentions |
RRID:Addgene_26756 | CFP, mCherry, and cI-YFP | Kanamycin | PMID:19029898 | Backbone Marker:Elowitz Lab; Backbone Size:3700; Vector Backbone:pZS21-cIYFP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-29 01:03:10 | 1 |
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