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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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mRFP-RBD(R59A)-mRFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_18664 | Ras binding domain of Raf | Ampicillin | PMID:16429133 | Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | R59A mutation in RBD | 2026-09-01 09:32:32 | 5 | ||
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mEGFP-HRas S17N Resource Report Resource Website 1+ mentions |
RRID:Addgene_18665 | H-Ras | Homo sapiens | Ampicillin | PMID:16429133 | Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S17N mutation in H-Ras | 2026-09-01 09:32:32 | 7 | |
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pEZY19 Resource Report Resource Website 1+ mentions |
RRID:Addgene_18668 | Chloramphenicol and Ampicillin | PMID:18064608 | pET19b Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal His tag. Note that the Addgene verified sequence detects a mutation in the enterokinase site D(V)DDK. | Backbone Marker:Novagen; Backbone Size:7934; Vector Backbone:pET19b; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-09-01 09:32:33 | 2 | |||
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pEZYegfp Resource Report Resource Website 10+ mentions |
RRID:Addgene_18671 | Chloramphenicol and Kanamycin | PMID:18064608 | pEGFPC4 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal EGFP tag. | Backbone Size:7040; Vector Backbone:pEGFPC4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin | 2026-09-01 09:32:33 | 10 | |||
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T4 Lysozyme T157E Resource Report Resource Website 1+ mentions |
RRID:Addgene_18640 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | T157E | 2026-09-01 09:32:28 | 2 | |
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scramble shRNA Resource Report Resource Website 500+ mentions |
RRID:Addgene_1864 | scramble | Ampicillin | PMID:15718470 | shRNA for use as a negative control. Sequence of hairpin is:CCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGG. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454). Please note that the 5' cloning site, AgeI, is typically destroyed during the shRNA cloning. Depending on the specific shRNA sequence, the site can occasionally be restored. AgeI is present in this plasmid. | Backbone Marker:Stewart SA, RNA 2003 Apr; 9(4):493-501.; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-09-01 09:32:28 | 693 | ||
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pMXs-gw Resource Report Resource Website 10+ mentions |
RRID:Addgene_18656 | Gateway cassette A | Chloramphenicol and Ampicillin | PMID:16904174 | The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC. | Backbone Size:4750; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-09-01 09:32:31 | 17 | ||
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pCMV/myc/Nuc-hOGG1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_18709 | 8-oxoguanine DNA glycosylase | Homo sapiens | Ampicillin | PMID:17018150 | Backbone Size:5000; Vector Backbone:pMCV/myc/nuc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | N/A | 2026-09-01 09:32:39 | 2 | |
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MSCV Luciferase PGK-hygro Resource Report Resource Website 10+ mentions |
RRID:Addgene_18782 | Ampicillin | Backbone Size:0; Vector Backbone:MSCV Luciferase PGK Hygro; Vector Types:Mammalian Expression, Retroviral, Luciferase; Bacterial Resistance:Ampicillin | 2026-09-01 09:32:50 | 34 | |||||
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pMN cFKT Resource Report Resource Website 1+ mentions |
RRID:Addgene_18785 | TRAP | Bacillus subtilis | Ampicillin | PMID:17023487 | Mammalian expression vector for the expression of HA and His6 epitope-tagged TRAP. There is a single amino acid (glycine) insertion between translational initiator codon and second amino acid of TRAP (asparagine) to maintain Kozak consensus sequence. Also, there is a single amino acid difference at amino acid #60 which has an asparagine (N) as in Bacillus amyloliquefaciens rather than glutamic acid (E) as in Bacillus subtilis. Codons are a mixture of human, Bacillus amyloliquefaciens, and Bacillus subtilis. | Backbone Size:4200; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:32:51 | 1 | |
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pTBS 5Y YIC Resource Report Resource Website 1+ mentions |
RRID:Addgene_18786 | TRAP binding site | Kanamycin | PMID:17023487 | An oligonucleotide with a 55-nt TRAP-binding sequence, TBS, was inserted into the 5'UTR of pYIC DNA to generate a bicistronic reporter gene with TBS: The TBS was inserted in the SacI site for 5-UTR (pTBS/5Y-YIC; 45 nt downstream of transcription start site and 9 nt upstream of YFP translational start site). | Backbone Marker:Available at Addgene (#18673); Backbone Size:6270; Vector Backbone:pYIC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-01 09:32:51 | 1 | ||
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pEZYflag Resource Report Resource Website 10+ mentions |
RRID:Addgene_18700 | Chloramphenicol and Ampicillin | PMID:18064608 | pFLAG-CMV-2 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal FLAG tag. | Backbone Marker:Sigma; Backbone Size:6932; Vector Backbone:pFlag-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-09-01 09:32:38 | 13 | |||
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pFLAG-Egfr(Velvet) Resource Report Resource Website 1+ mentions |
RRID:Addgene_18788 | epidermal growth factor receptor | Mus musculus | Ampicillin | PMID:15020428 | The EGFR internal signal sequence was removed during the cloning of this construct. When compared to GenBank reference sequence NP_997538.1, the signal sequence corresponds to amino acids 1-24 | Backbone Marker:Sigma; Backbone Size:6280; Vector Backbone:pFLAG-CMV3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D833G; signal sequence removed (aa1-24) | 2026-09-01 09:32:51 | 2 |
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pEZYmyc-His Resource Report Resource Website 1+ mentions |
RRID:Addgene_18701 | Chloramphenicol and Ampicillin | PMID:18064608 | pcDNA3.1(-)/myc-His B Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an C-terminal myc-His tag. | Backbone Marker:Invitrogen; Backbone Size:7755; Vector Backbone:pcDNA3.1(-)/Myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-09-01 09:32:38 | 6 | |||
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pEZY45 Resource Report Resource Website 1+ mentions |
RRID:Addgene_18704 | Chloramphenicol and Ampicillin | PMID:18064608 | pJG4-5 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a prey vector expression clone for yeast two-hybrid. | Backbone Size:8718; Vector Backbone:pJG4-5; Vector Types:Yeast Expression, two-hybrid prey vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-09-01 09:32:38 | 1 | |||
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pEZY202 Resource Report Resource Website 1+ mentions |
RRID:Addgene_18705 | Chloramphenicol and Ampicillin | PMID:18064608 | pEG202 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a bait vector expression clone for yeast two-hybrid. | Backbone Size:12414; Vector Backbone:pEG202; Vector Types:Yeast Expression, two-hybrid bait vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-09-01 09:32:39 | 1 | |||
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cytoplasmic EKAR (EGFP-mRFP) Resource Report Resource Website 1+ mentions |
RRID:Addgene_18680 | ERK Activity Reporter | Ampicillin | PMID:19033456 | Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:32:34 | 9 | |||
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pYIC Resource Report Resource Website 1+ mentions |
RRID:Addgene_18673 | Bicistronic fluorescent reporter gene with cap-dependent EYFP and IRES-dependent ECFP translation under the control of CMV promo | Homo sapiens | Kanamycin | PMID:17023487 | EYFP tagged at N-terminus with 3 copies of Myc epitope tags and at C-terminus with a copy of FLAG and polyhistidine (His6) epitope tags. ECFP tagged at the C-terminus with one copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags. EGFP of pIRES2-EGFP (Clontech) replaced at an 803 bp BstXI-NotI, PCR amplified fragment with ECFP from pECFP-Mito (Clontech) tagged at the C-terminus with a single copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags; IRES-dependent translation yields ECFP-HA-His6 fusion protein. 895 bp Sac I/Eco RI fragment containing amino terminal 3 Myc epitope tags fused to EYFP obtained from PCR amplification of pEYFP-N1 (Clontech), which is also tagged at C-terminus with a single copy of FLAG and polyhistidine (His6) epitope tag; cap-dependent translation yields 3Myc-EYFP-Flag-His6 fusion protein. See article for detailed information. | Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-01 09:32:33 | 9 | |
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HA-NEDD8 Resource Report Resource Website 1+ mentions |
RRID:Addgene_18711 | NEDD8 | Homo sapiens | Ampicillin | PMID:9353319 | Note that this is the active form of NEDD8. Pro-NEDD8 (which is inactive) has an additional 5 amino acids at the c-terminus. | Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:32:40 | 8 | |
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HA-Ubiquitin Resource Report Resource Website 100+ mentions |
RRID:Addgene_18712 | ubiquitin | Homo sapiens | Ampicillin | PMID:9353319 | This plasmid grows slowly. We encourage requesting scientists to grow for a longer period of time than normal. Please note that this plasmid runs as a dimer (>10kb) or multimer. | Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | The HA-Ubiquitin insert is from the UBB gene. The reason for the small size is because it contains a single ubiquitin coding sequence(full length UBB has three repeating ubiquitin coding sequences). | 2026-09-01 09:32:40 | 215 |
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