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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
mRFP-RBD(R59A)-mRFP
 
Resource Report
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1+ mentions
RRID:Addgene_18664 Ras binding domain of Raf Ampicillin PMID:16429133 Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin R59A mutation in RBD 2026-09-01 09:32:32 5
mEGFP-HRas S17N
 
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1+ mentions
RRID:Addgene_18665 H-Ras Homo sapiens Ampicillin PMID:16429133 Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S17N mutation in H-Ras 2026-09-01 09:32:32 7
pEZY19
 
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1+ mentions
RRID:Addgene_18668 Chloramphenicol and Ampicillin PMID:18064608 pET19b Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal His tag. Note that the Addgene verified sequence detects a mutation in the enterokinase site D(V)DDK. Backbone Marker:Novagen; Backbone Size:7934; Vector Backbone:pET19b; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-09-01 09:32:33 2
pEZYegfp
 
Resource Report
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10+ mentions
RRID:Addgene_18671 Chloramphenicol and Kanamycin PMID:18064608 pEGFPC4 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal EGFP tag. Backbone Size:7040; Vector Backbone:pEGFPC4; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin 2026-09-01 09:32:33 10
T4 Lysozyme T157E
 
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1+ mentions
RRID:Addgene_18640 T4 Lysozyme Bacteriophage T4 Ampicillin The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T157E 2026-09-01 09:32:28 2
scramble shRNA
 
Resource Report
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500+ mentions
RRID:Addgene_1864 scramble Ampicillin PMID:15718470 shRNA for use as a negative control. Sequence of hairpin is:CCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGG. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454). Please note that the 5' cloning site, AgeI, is typically destroyed during the shRNA cloning. Depending on the specific shRNA sequence, the site can occasionally be restored. AgeI is present in this plasmid. Backbone Marker:Stewart SA, RNA 2003 Apr; 9(4):493-501.; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-09-01 09:32:28 693
pMXs-gw
 
Resource Report
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10+ mentions
RRID:Addgene_18656 Gateway cassette A Chloramphenicol and Ampicillin PMID:16904174 The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC. Backbone Size:4750; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-09-01 09:32:31 17
pCMV/myc/Nuc-hOGG1
 
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1+ mentions
RRID:Addgene_18709 8-oxoguanine DNA glycosylase Homo sapiens Ampicillin PMID:17018150 Backbone Size:5000; Vector Backbone:pMCV/myc/nuc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin N/A 2026-09-01 09:32:39 2
MSCV Luciferase PGK-hygro
 
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10+ mentions
RRID:Addgene_18782 Ampicillin Backbone Size:0; Vector Backbone:MSCV Luciferase PGK Hygro; Vector Types:Mammalian Expression, Retroviral, Luciferase; Bacterial Resistance:Ampicillin 2026-09-01 09:32:50 34
pMN cFKT
 
Resource Report
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1+ mentions
RRID:Addgene_18785 TRAP Bacillus subtilis Ampicillin PMID:17023487 Mammalian expression vector for the expression of HA and His6 epitope-tagged TRAP. There is a single amino acid (glycine) insertion between translational initiator codon and second amino acid of TRAP (asparagine) to maintain Kozak consensus sequence. Also, there is a single amino acid difference at amino acid #60 which has an asparagine (N) as in Bacillus amyloliquefaciens rather than glutamic acid (E) as in Bacillus subtilis. Codons are a mixture of human, Bacillus amyloliquefaciens, and Bacillus subtilis. Backbone Size:4200; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:32:51 1
pTBS 5Y YIC
 
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1+ mentions
RRID:Addgene_18786 TRAP binding site Kanamycin PMID:17023487 An oligonucleotide with a 55-nt TRAP-binding sequence, TBS, was inserted into the 5'UTR of pYIC DNA to generate a bicistronic reporter gene with TBS: The TBS was inserted in the SacI site for 5-UTR (pTBS/5Y-YIC; 45 nt downstream of transcription start site and 9 nt upstream of YFP translational start site). Backbone Marker:Available at Addgene (#18673); Backbone Size:6270; Vector Backbone:pYIC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:32:51 1
pEZYflag
 
Resource Report
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10+ mentions
RRID:Addgene_18700 Chloramphenicol and Ampicillin PMID:18064608 pFLAG-CMV-2 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal FLAG tag. Backbone Marker:Sigma; Backbone Size:6932; Vector Backbone:pFlag-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-09-01 09:32:38 13
pFLAG-Egfr(Velvet)
 
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1+ mentions
RRID:Addgene_18788 epidermal growth factor receptor Mus musculus Ampicillin PMID:15020428 The EGFR internal signal sequence was removed during the cloning of this construct. When compared to GenBank reference sequence NP_997538.1, the signal sequence corresponds to amino acids 1-24 Backbone Marker:Sigma; Backbone Size:6280; Vector Backbone:pFLAG-CMV3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin D833G; signal sequence removed (aa1-24) 2026-09-01 09:32:51 2
pEZYmyc-His
 
Resource Report
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1+ mentions
RRID:Addgene_18701 Chloramphenicol and Ampicillin PMID:18064608 pcDNA3.1(-)/myc-His B Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an C-terminal myc-His tag. Backbone Marker:Invitrogen; Backbone Size:7755; Vector Backbone:pcDNA3.1(-)/Myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-09-01 09:32:38 6
pEZY45
 
Resource Report
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1+ mentions
RRID:Addgene_18704 Chloramphenicol and Ampicillin PMID:18064608 pJG4-5 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a prey vector expression clone for yeast two-hybrid. Backbone Size:8718; Vector Backbone:pJG4-5; Vector Types:Yeast Expression, two-hybrid prey vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-09-01 09:32:38 1
pEZY202
 
Resource Report
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1+ mentions
RRID:Addgene_18705 Chloramphenicol and Ampicillin PMID:18064608 pEG202 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a bait vector expression clone for yeast two-hybrid. Backbone Size:12414; Vector Backbone:pEG202; Vector Types:Yeast Expression, two-hybrid bait vector; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-09-01 09:32:39 1
cytoplasmic EKAR (EGFP-mRFP)
 
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1+ mentions
RRID:Addgene_18680 ERK Activity Reporter Ampicillin PMID:19033456 Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:32:34 9
pYIC
 
Resource Report
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1+ mentions
RRID:Addgene_18673 Bicistronic fluorescent reporter gene with cap-dependent EYFP and IRES-dependent ECFP translation under the control of CMV promo Homo sapiens Kanamycin PMID:17023487 EYFP tagged at N-terminus with 3 copies of Myc epitope tags and at C-terminus with a copy of FLAG and polyhistidine (His6) epitope tags. ECFP tagged at the C-terminus with one copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags. EGFP of pIRES2-EGFP (Clontech) replaced at an 803 bp BstXI-NotI, PCR amplified fragment with ECFP from pECFP-Mito (Clontech) tagged at the C-terminus with a single copy of hemagglutinin (HA) and polyhistidine (His6) epitope tags; IRES-dependent translation yields ECFP-HA-His6 fusion protein. 895 bp Sac I/Eco RI fragment containing amino terminal 3 Myc epitope tags fused to EYFP obtained from PCR amplification of pEYFP-N1 (Clontech), which is also tagged at C-terminus with a single copy of FLAG and polyhistidine (His6) epitope tag; cap-dependent translation yields 3Myc-EYFP-Flag-His6 fusion protein. See article for detailed information. Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-09-01 09:32:33 9
HA-NEDD8
 
Resource Report
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1+ mentions
RRID:Addgene_18711 NEDD8 Homo sapiens Ampicillin PMID:9353319 Note that this is the active form of NEDD8. Pro-NEDD8 (which is inactive) has an additional 5 amino acids at the c-terminus. Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-01 09:32:40 8
HA-Ubiquitin
 
Resource Report
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100+ mentions
RRID:Addgene_18712 ubiquitin Homo sapiens Ampicillin PMID:9353319 This plasmid grows slowly. We encourage requesting scientists to grow for a longer period of time than normal. Please note that this plasmid runs as a dimer (>10kb) or multimer. Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin The HA-Ubiquitin insert is from the UBB gene. The reason for the small size is because it contains a single ubiquitin coding sequence(full length UBB has three repeating ubiquitin coding sequences). 2026-09-01 09:32:40 215

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