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Species: Synthetic
Genetic Insert: 100 bp barcoding cassette
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6599; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36893271
Comments: After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes
Proper citation: RRID:Addgene_199129 Copy
Species: Synthetic
Genetic Insert: 100 bp barcoding cassette
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6599; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36893271
Comments: After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes
Proper citation: RRID:Addgene_199124 Copy
Species: Synthetic
Genetic Insert: 100 bp barcoding cassette
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6599; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36893271
Comments: After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes
Proper citation: RRID:Addgene_199125 Copy
Species: Synthetic
Genetic Insert: miRFP718nano(S86C)
Vector Backbone Description: Backbone Size:10120; Vector Backbone:pCSIIbleo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_197373 Copy
Species: Synthetic
Genetic Insert: CVB1 VP1 protein with Histag
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37227147
Comments: Insert cloned to plasmid with BamHI (5') and EcoRI (3') restriction
Proper citation: RRID:Addgene_202069 Copy
Species: Synthetic
Genetic Insert: CVA4 VP1 protein with Histag
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37227147
Comments: Insert cloned to plasmid with BamHI (5') and EcoRI (3') restriction
Proper citation: RRID:Addgene_202068 Copy
Species: Synthetic
Genetic Insert: E30 VP1 protein with Histag
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37227147
Comments: Insert cloned to plasmid with BamHI (5') and EcoRI (3') restriction
Proper citation: RRID:Addgene_202070 Copy
Species: Synthetic
Genetic Insert: SCAT3NES
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pPBbsr2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37519219
Proper citation: RRID:Addgene_197350 Copy
Species: Synthetic
Genetic Insert: 100 bp barcoding cassette
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6599; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36893271
Comments: After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes
Proper citation: RRID:Addgene_199138 Copy
Species: Synthetic
Genetic Insert: 100 bp barcoding cassette
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6599; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36893271
Comments: After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes
Proper citation: RRID:Addgene_199133 Copy
Species: Synthetic
Genetic Insert: 100 bp barcoding cassette
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6599; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36893271
Comments: After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes
Proper citation: RRID:Addgene_199134 Copy
Species: Synthetic
Genetic Insert: 100 bp barcoding cassette
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6599; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36893271
Comments: After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes
Proper citation: RRID:Addgene_199143 Copy
Species: Synthetic
Genetic Insert: 100 bp barcoding cassette
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6599; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36893271
Comments: After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes
Proper citation: RRID:Addgene_199140 Copy
Species: Synthetic
Genetic Insert: NIR-GECO2(C18S/I426C)
Vector Backbone Description: Backbone Size:10123; Vector Backbone:pCSIIbleo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_197368 Copy
Species: Synthetic
Genetic Insert: 100 bp barcoding cassette
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:6599; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36893271
Comments: After restriction digestion with NgoMIV, this plasmid can be used to transform Candida strains which would barcode it with unique DNA barcodes
Proper citation: RRID:Addgene_199145 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:K4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204718 Copy
Species: Synthetic
Genetic Insert: Optimized neurogenin-2
Vector Backbone Description: Vector Backbone:K4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204734 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204732 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV.CAP-Mac VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37430038
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
This work was supported by funding from:
NIH BRAIN Armamentarium UF1MH128336
Michael J Fox Foundation for Parkinson's Research ASAP-020495
Proper citation: RRID:Addgene_200658 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204730 Copy
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